CAJ | 학술논문

免疫细胞过继性治疗是一种新的肿瘤治疗技术，是对传统的手术、化疗、放疗等肿瘤治疗方法的有力补充。细胞因子诱导杀伤(CIK)细胞是应用最为广泛的免疫细胞之一，其克服了以往免疫治疗细胞在体外扩增难、对肿瘤的细胞毒性不强和可能潜在致癌性等缺陷。CIK细胞是体外扩增的异质淋巴细胞，具有非MHC限制的抗肿瘤活性，主要的效应细胞是表达CD3+CD56+的NK样T细胞。虽然，到目前为止，CIK细胞的杀伤机制尚不完全清楚，但NKG2D及其配体的相互作用在CIK抗肿瘤中发挥了重要作用。大量的临床试验证明了CIK细胞治疗的可行性、有效性和安全性：CIK细胞体外培养简单，扩增能力强，容易获得足够的细胞；治疗效果明显，多数患者病情得到缓解，免疫功能提高，生活质量评分提高；副作用少且轻微，如恶心、呕吐、低烧、发抖等，都可以通过简单的治疗得到缓解。本文综述了CIK细胞的生物学特性、杀伤机制、动物实验、临床应用、以及应用CIK细胞治疗的开拓研究等方面的研究进展。
면역세포과계성치료시일충신적종류치료기술，시대전통적수술、화료、방료등종류치료방법적유력보충。세포인자유도살상(CIK)세포시응용최위엄범적면역세포지일，기극복료이왕면역치료세포재체외확증난、대종류적세포독성불강화가능잠재치암성등결함。CIK세포시체외확증적이질림파세포，구유비MHC한제적항종류활성，주요적효응세포시표체CD3+CD56+적NK양T세포。수연，도목전위지，CIK세포적살상궤제상불완전청초，단NKG2D급기배체적상호작용재CIK항종류중발휘료중요작용。대량적림상시험증명료CIK세포치료적가행성、유효성화안전성：CIK세포체외배양간단，확증능력강，용역획득족구적세포；치료효과명현，다수환자병정득도완해，면역공능제고，생활질량평분제고；부작용소차경미，여악심、구토、저소、발두등，도가이통과간단적치료득도완해。본문종술료CIK세포적생물학특성、살상궤제、동물실험、림상응용、이급응용CIK세포치료적개탁연구등방면적연구진전。
Adoptive immune cell therapy is a powerful complement to the traditional cancer treatments such as sur-gery, chemotherapy, radiotherapy and so on. Cytokine-induced killer (CIK) cell is one of the most widely used immune cells. There are some defects in the earlier immune cells treatments, such as difficulty to amplify in vitro, low cytotoxicity against tumors, potential carcinogenicity and so on. However, all these defects can be overcome by using CIK cells. CIK cells are heterogeneous T lymphocytes expanded in vitro, endowed with a wide MHC-unrestricted antitumor activity. The CD3+CD56+NK-like T cells are the main effect cells for antitumor activity of CIK cells. Although the mechanism against tumors by CIK cells has not been fully clarified, the interaction of NKG2D receptor and its ligands on CIK cells plays a main role in the treatment of tumor. A lot of clinical trials have demonstrated that the treatments with CIK cells are feasible, ef-fective and safe. In vitro, CIK cells are easy to culture and show a strong proliferative capacity. Therefore, it is easy to gain enough cells by proliferating to satisfy clinical requirements. The effects of treatments using CIK cell are obvious, as the immune function is improved, and the scores of quality of life are higher in the patients with CIK treatment than those with traditional treatments. The adverse effects are less and weak, such as nausea, vomiting, fever, shivering and so on, all are easy to get remission after simple treatment. In this paper, the research progresses of biological characteristics, anti-tumor mechanisms, animal experiments, clinical application of CIK cells and groundbreaking study on the CIK treatment are mainly reviewed.