CAJ | 학술논문

目的：探讨去氧胆酰酪氨酸（D CT）对人类精子活力、质膜完整性以及凋亡的影响。方法上游高活力人类精子分别与DCT 0．25，0．5和1．0 g·L-1作用20 min，取精子悬液分析精子活率（SM）和活力（SV）；SYBR-14/PI双染后经过荧光显微镜和流式细胞仪观察质膜完整性，AnnexinⅤ/PI双染流式细胞仪分析细胞凋亡。结果 DCT对SM和SV均有显著的抑制作用，且存在量-效关系（rSV＝0．91，rSM＝0．88， P＜0．05）。质膜完整性结果显示，质膜破损精子在DCT 0．25，0．5和1．0 g·L-1时质膜破损精子百分率分别为（54．3±9．8）％，（73．8±3．5）％和（80．6±8．3）％，与空白对照组具有明显差别（P＜0．05），且具有明显的量效关系（r＝0．91，P＜0．05）。凋亡实验结果显示，晚期凋亡精子百分率在0．25，0．5和1．0 g·L-1时，分别是（11．9±1．2）％，（30．1±6．3）％和（75．6±6．3）％，具有明显的浓度依赖性（r＝0．98，P＜0．05）。DCT≤0．5g·L-1引起精子早期凋亡率升高，但是高浓度时却出现降低趋势。结论 DCT不仅破坏精子质膜完整性，同时诱发精子凋亡，导致受精能力降低或消失。
목적：탐토거양담선락안산（D CT）대인류정자활력、질막완정성이급조망적영향。방법상유고활력인류정자분별여DCT 0．25，0．5화1．0 g·L-1작용20 min，취정자현액분석정자활솔（SM）화활력（SV）；SYBR-14/PI쌍염후경과형광현미경화류식세포의관찰질막완정성，AnnexinⅤ/PI쌍염류식세포의분석세포조망。결과 DCT대SM화SV균유현저적억제작용，차존재량-효관계（rSV＝0．91，rSM＝0．88， P＜0．05）。질막완정성결과현시，질막파손정자재DCT 0．25，0．5화1．0 g·L-1시질막파손정자백분솔분별위（54．3±9．8）％，（73．8±3．5）％화（80．6±8．3）％，여공백대조조구유명현차별（P＜0．05），차구유명현적량효관계（r＝0．91，P＜0．05）。조망실험결과현시，만기조망정자백분솔재0．25，0．5화1．0 g·L-1시，분별시（11．9±1．2）％，（30．1±6．3）％화（75．6±6．3）％，구유명현적농도의뢰성（r＝0．98，P＜0．05）。DCT≤0．5g·L-1인기정자조기조망솔승고，단시고농도시각출현강저추세。결론 DCT불부파배정자질막완정성，동시유발정자조망，도치수정능력강저혹소실。
OBJECTIVE Toexploretheeffectofdeoxycholytyosine(DCT)onhumanspermviabili-ty,plasmamembraneintegrityandapoptosis.METHODS SpermswereincubatedwithDCT0,0.25, 0.5,or 1 .0 g·L-1 for 20 min.The treated sperm suspension was detected by the CASA system to measure the sperm viability (SV)and sperm motility (SM).The treated sperm suspension was also incubated with SYBR-1 4/PI and exa mined by epifluorescence microscopy and flow cyto metry (FCM). The same treated sperm suspension was cultured by AnnexinⅤ/PI and detected by FCM.RESULTS The sperm viability and motility were significantly inhibited by DCT in a concentration-dependent manner (rSV=0.91 ,rSM=0.88,P<0.05).According to the results of sperm plasma membrane tests,the per-centage of dead sperm in DCT 0.25,0.5,and 1 .0 g·L-1 was (54.3 ±9.8)%,(73.8 ±3.5)% and (80.6 ±8.3)% respectively and had an obvious concentration-effect relationship(r=0.91 ,P<0.05). Apoptosis test results showed that the percentage of apoptotic sperm in DCT 0.25,0.5,and 1 .0 g·L-1 was (1 1 .9 ±1 .2)%,(30.1 ±6.3)% and (75.6 ±6.3)% respectively,which also had an obvious con-centration-effect relationship and was significantly different from the control group(P<0.05).The rate of early-stage apoptosis of sperm increased with DCT at a low concentration(≤0.5 g·L-1 ),but decreased atahighDCTconcentration.CONCLUSION DCTcannotonlydestroythespermplasmamembrane but also induce sperm apoptosis,having an adverse effect on the fertilization ability of sperm.