CAJ | 학술논문

目的：探讨加味四妙丸（MSW）有效部位群（EFC）对高尿酸血症大鼠血清尿酸水平的影响及其作用机制。方法采用由次黄嘌呤和尿酸酶抑制剂氧嗪酸钾制备持续性大鼠高尿酸血症模型，记录每日摄食量，收集末次造模后12 h尿液，ig给予模型大鼠MSW 50 g·kg -1以及EFC 12．5，25和50 g·kg -1，连续5 d；采用尿酸排泄抑制剂烟酸制备高尿酸血症模型，ig 给予模型大鼠 MSW 50 g·kg -1以及 EFC 50 g·kg -1，连续3 d。末次给药后，取血和肝组织。全自动生化仪检测大鼠血清和尿液中尿酸水平，酶比色法测定血清、肝组织中黄嘌呤氧化酶（XOD）、分光光度法检测红细胞、肝组织中嘌呤核苷磷酸化酶（PNP）和尿酸酶等活性。结果与正常对照组相比，2种高尿酸血症模型大鼠血清尿酸水平均明显升高（P＜0．01），MSW和EFC 50 g·kg -1均可显著降低2种模型大鼠血尿酸水平（P＜0．01）。EFC 12．5和25 g·kg -1可降低由氧嗪酸钾制备的持续性高尿酸血症大鼠血尿酸水平（P＜0．05），MSW和EFC各剂量组均可明显升高模型动物红细胞PNP活性（P＜0．01），EFC 25和50 g·kg -1还可显著降低血清XOD 活性（P＜0．05，P＜0．01）。对由烟酸复制的大鼠高尿酸血症模型，MSW 50 g·kg -1和EFC 50 g·kg -1均能升高肝组织尿酸酶活性（P＜0．05），EFC 50g·kg-1能显著降低模型大鼠血清中XOD活性（P＜0．01）。结论 EFC能降低高尿酸血症模型大鼠的尿酸水平，其机制可能与其抑制血清XOD活性使尿酸合成减少以及激活尿酸酶活性促进尿酸分解有关。
목적：탐토가미사묘환（MSW）유효부위군（EFC）대고뇨산혈증대서혈청뇨산수평적영향급기작용궤제。방법채용유차황표령화뇨산매억제제양진산갑제비지속성대서고뇨산혈증모형，기록매일섭식량，수집말차조모후12 h뇨액，ig급여모형대서MSW 50 g·kg -1이급EFC 12．5，25화50 g·kg -1，련속5 d；채용뇨산배설억제제연산제비고뇨산혈증모형，ig 급여모형대서 MSW 50 g·kg -1이급 EFC 50 g·kg -1，련속3 d。말차급약후，취혈화간조직。전자동생화의검측대서혈청화뇨액중뇨산수평，매비색법측정혈청、간조직중황표령양화매（XOD）、분광광도법검측홍세포、간조직중표령핵감린산화매（PNP）화뇨산매등활성。결과여정상대조조상비，2충고뇨산혈증모형대서혈청뇨산수평균명현승고（P＜0．01），MSW화EFC 50 g·kg -1균가현저강저2충모형대서혈뇨산수평（P＜0．01）。EFC 12．5화25 g·kg -1가강저유양진산갑제비적지속성고뇨산혈증대서혈뇨산수평（P＜0．05），MSW화EFC각제량조균가명현승고모형동물홍세포PNP활성（P＜0．01），EFC 25화50 g·kg -1환가현저강저혈청XOD 활성（P＜0．05，P＜0．01）。대유연산복제적대서고뇨산혈증모형，MSW 50 g·kg -1화EFC 50 g·kg -1균능승고간조직뇨산매활성（P＜0．05），EFC 50g·kg-1능현저강저모형대서혈청중XOD활성（P＜0．01）。결론 EFC능강저고뇨산혈증모형대서적뇨산수평，기궤제가능여기억제혈청XOD활성사뇨산합성감소이급격활뇨산매활성촉진뇨산분해유관。
OBJECTIVE Tostudytheeffectofeffectivefractions(EFC)frommodifiedSimiaoWan (MSW)onthelevelofuricacidinhyperuricemicratsandinvestigatethemechanism.METHODS Two types of hyperurice mic models were established.A persistant hyperurice mic model was prepared by giving rats oxonic acid 200 mg·kg -1 and feeding the m with hypoxanthine.The models were ig given with modified Simiaowan (MSW)50 g·kg -1 or EFC 1 2.5,25 and 50 g·kg -1 consecutively for 5 d.The models were treated with MSW or EFC 50 g·kg -1 for 3 d.After the final treatment,the uric acid concen-trations in seru m and urine were determined by an auto matic bioche mistry analyzer.The activity of xan-thine oxidase (XOD )in the serum and liver was determined by enzymic colorimetric method.The activity of purine nucleoside phosphorylase (PNP)and uricase was detected by spectrophotometry. RESULTS Comparedwithnormalcontrolgroup,theserumlevelofuricacidinbothmodelgroupswas remarkably increased(P<0.01 ).Compared to model control group,MSW 50 g·kg -1 and EFC 12.5, 25 and 50 g·kg -1 significantly reduced the serum level of uric acid(P<0.05,P<0.01 ),but increased the activity of erythrocyte PNP(P<0.01 )in the oxonic acid potassium-induced hyperuricemia rats. MSW 50 g·kg -1 and EFC 50 g·kg -1 elevated the activity of liver uricase in the nicotinic acid-induced hyperuricemia rats(P<0.05).EFC 50 g·kg -1 also significantly decreased the serum XOD activity of hyperuricemicrats.CONCLUSION EFCsignificantlyinhibitstheserumlevelofuricacidinhyperurice-mic rats,which might involve down-regulation of protein levels of serum XOD to inhibit the production of uric acid and activation of uricase to pro mote the deco mposition of uric acid.