CAJ | 학술논문

在前期试验中分离到对柑橘病害具有生防作用的土壤放线菌ML27，为进一步了解该菌的生防作用，对其进行了鉴定和抗真菌活性代谢产物的性质研究。形态特征、培养特性、生理生化特性和16S rDNA序列分析表明，该放线菌被归为黄色类群链霉菌（ Streptomyces sp．）；发酵滤液活性测定结果表明，菌株ML27抗真菌活性代谢产物在酸性环境下稳定，对温度不敏感，紫外线稳定性较好，胰蛋白酶对发酵滤液活性无明显影响；不同极性有机溶剂萃取后生测表明其主要抗真菌活性代谢产物为脂溶性。试验结果可为菌株ML27防治柑橘真菌性病害和开发具有自主知识产权的生物杀菌剂奠定基础。
재전기시험중분리도대감귤병해구유생방작용적토양방선균ML27，위진일보료해해균적생방작용，대기진행료감정화항진균활성대사산물적성질연구。형태특정、배양특성、생리생화특성화16S rDNA서렬분석표명，해방선균피귀위황색류군련매균（ Streptomyces sp．）；발효려액활성측정결과표명，균주ML27항진균활성대사산물재산성배경하은정，대온도불민감，자외선은정성교호，이단백매대발효려액활성무명현영향；불동겁성유궤용제췌취후생측표명기주요항진균활성대사산물위지용성。시험결과가위균주ML27방치감귤진균성병해화개발구유자주지식산권적생물살균제전정기출。
Actinomyces ML27 from soil,which is able to control the disease of citrus ,was obtained in the previous experiment .To clarify the function of ML 27 against citrus diseases , the identification and antifungal bioactive metabolites produced by ML 27 were executed .By the methods of morphological ,chemical and 16S rD-NA gene sequenced and analyzed for similarities ,strain ML27 was indentified as Streptomyces sp..The result from detecting the activity of the fermentation liquid indicated that the antifungal substances were not sensitive to the temperature ,and stable under acidic pH/ultraviolet radiation/proteases .It was found that the main anti-fungal activity substances were soluble in grease of different organics .The results can be for establishing the foundation on applying the strain ML 27 for controling the fungal diseases on citrus and exploring the biological fungicides with own intellectual property rights .