CAJ | 학술논문

类胰岛素生长因子（Insulin-like grow factor，IGFs）与动物生长发育呈强正相关，为了填补鸭胚胎期类胰岛素生长因子受体（ Insulin-like growth factor-I receptors ，IGF-IR）在骨骼肌发育变化的研究空白，实验采用qRT-PCR研究生长速度差异较大的高邮鸭和金定鸭胚胎期（13、15、17、19、21和23胚龄）骨骼肌（胸肌和腿肌）成肌细胞IGF-IR基因发育性表达的差异。研究显示，成肌细胞IGF-IR mRNA在2个品种的各发育时间都表达，种间同一组织表达量变化趋势较一致，总体均呈现先升后降的趋势。胸肌中，基因表达量在17胚龄时达到最高峰，显著高于其他胚龄（ P＜0．05）；腿肌中13胚龄与17胚龄都显著高于其他胚龄（ P＜0．05）；品种间除了金定鸭17胚龄时胸肌IGF-IR mRNA的表达量极显著高于高邮鸭（ P＜0．01），其他各胚龄差异均不显著。不同肌肉组织的IGF-IR mRNA表达变化存在种间和空间差异性。不同肌肉组织成肌细胞中的IGF-IR基因表达具有显著差异，与对应组织中的表达不一致，揭示在研究转录分析时应从不同层次进行比较研究，以期获得比较系统的科学支撑。
류이도소생장인자（Insulin-like grow factor，IGFs）여동물생장발육정강정상관，위료전보압배태기류이도소생장인자수체（ Insulin-like growth factor-I receptors ，IGF-IR）재골격기발육변화적연구공백，실험채용qRT-PCR연구생장속도차이교대적고유압화금정압배태기（13、15、17、19、21화23배령）골격기（흉기화퇴기）성기세포IGF-IR기인발육성표체적차이。연구현시，성기세포IGF-IR mRNA재2개품충적각발육시간도표체，충간동일조직표체량변화추세교일치，총체균정현선승후강적추세。흉기중，기인표체량재17배령시체도최고봉，현저고우기타배령（ P＜0．05）；퇴기중13배령여17배령도현저고우기타배령（ P＜0．05）；품충간제료금정압17배령시흉기IGF-IR mRNA적표체량겁현저고우고유압（ P＜0．01），기타각배령차이균불현저。불동기육조직적IGF-IR mRNA표체변화존재충간화공간차이성。불동기육조직성기세포중적IGF-IR기인표체구유현저차이，여대응조직중적표체불일치，게시재연구전록분석시응종불동층차진행비교연구，이기획득비교계통적과학지탱。
Insulin-like growth factor has a strong positive correlation with animal growth and development . In order to fill the gap in the research on the changes of insulin-like growth factor-I receptors ( IGF-IR) dur-ing the development of the skeletal muscle of duck , the IGF-IR mRNA expressions on the skeletal myoblast during the embryonic stage of Gaoyou duck and Jinding duck i .e.on day 13,15,17,19,21 and 23 were detec-ted using quantitative RT-PCR method in this experiment .The result indicated that the profiles of duck IGF-IR gene on myoblast IGF-IR mRNA in the two duck breeds were relatively consistent during the early develop-ment.On the whole the expression level of IGF-IR gene increased first and decreased afterwards and showed an analogous curves between the varieties .The highest level of IGF-IR mRNA appeared at the 17 embryo age in pectorals and at the 13 and 17 embryo ages in leg of both the breeds ( P<0.05) .The expression levels of IGF-IR mRNA in pectorals of Jinding duck were significantly higher than that of Gaoyou duck at the 17 embryo age ( P<0.01) and others had no significant difference ( P>0.05) between varieties.Conclusion:there were differ-ences between varieties and spaces in the expression of IGF-IR mRNA in different muscle myoblast .There were significant difference between different skeletal myoblasts and inconsistent with the corresponding tissues in the expression level of IGF-IR gene.So the transcriptional analysis of gene expression should focus on differ-ent layers in order to obtain a more systematic scientific support .