CAJ | 학술논문

以2，5二甲氧基苯磺酰氯（ DMOSC）为柱前衍生化试剂，建立了柱前衍生草甘膦的紫外检测反相高效液相色谱法，并优化了衍生化条件，得最佳条件：衍生温度35℃，时间15 min， pH 10?0，草甘膦与DMOSC的摩尔比为1∶6。HPLC分析条件：采用Kromasil C18柱，流速1?0 mL／min，柱温30℃，检测波长220 nm，流动相为甲醇-乙腈-磷酸盐缓冲溶液（0?02 mol／L、pH 5?5），三者的体积比为15∶5∶80。结果表明：草甘膦质量浓度在5～100μg／mL范围内线性关系良好，相关系数为0?9962，检测限为0?067μg／mL。实验表明该方法反应条件温和，灵敏度高，衍生产物稳定。
이2，5이갑양기분광선록（ DMOSC）위주전연생화시제，건립료주전연생초감련적자외검측반상고효액상색보법，병우화료연생화조건，득최가조건：연생온도35℃，시간15 min， pH 10?0，초감련여DMOSC적마이비위1∶6。HPLC분석조건：채용Kromasil C18주，류속1?0 mL／min，주온30℃，검측파장220 nm，류동상위갑순-을정-린산염완충용액（0?02 mol／L、pH 5?5），삼자적체적비위15∶5∶80。결과표명：초감련질량농도재5～100μg／mL범위내선성관계량호，상관계수위0?9962，검측한위0?067μg／mL。실험표명해방법반응조건온화，령민도고，연생산물은정。
A derivatization method for the determination of glyphosate had been developed by using 2,5-dimethoxybenzenesulfonyl chloride?The optimized conditions of derivatization were as follows:reaction temperature 35 ℃,reaction time 15 min,solution pH value 10?0,the buffer reaction concentration 0?2 mol/L,and ratio of n( glyphosate) ∶n( o-nitrobenzenesulfonyl)= 1∶6?The chromatographic conditions were as follows:Kromasil C18 column(5 μm,4?6 ×250 mm),detection wavelength 220 nm,flow rate 1?0 mL/min,mobile phase A ( methanol)∶mobile phase B ( acetonitrile)∶mobile phase C ( phosphate buffer solution(PBS,pH 5?5,0?02 mol/L))= 15∶5∶80,isocratic elution.The calibration curve for glyphosate was linear ranging from 5 μg/mL to 100 μg/mL. The linear correlation coefficient was 0?996 2. The limit of detection was 0?067 μg/mL?The experimental result shows that the method is simple,rapid and reliable,and generally applicable.