CAJ | 학술논문

以海泡石作为猪胰脂肪酶（ PPL）的固定化载体，考察采用物理吸附的方法制备固定化脂肪酶的条件。结果表明：在固载时间4 h、反应磷酸盐（ PBS）溶液pH 6?0、反应温度25℃时，可达最大比酶活309 U／g，固定化酶的化学稳定性和热稳定性均较高。同时利用红外谱仪（ FT IR）和扫描电子显微镜（ SEM）的分析手段对固定化猪胰脂肪酶试样进行分析，进一步确定了海泡石材料在固定化酶中的作用。
이해포석작위저이지방매（ PPL）적고정화재체，고찰채용물리흡부적방법제비고정화지방매적조건。결과표명：재고재시간4 h、반응린산염（ PBS）용액pH 6?0、반응온도25℃시，가체최대비매활309 U／g，고정화매적화학은정성화열은정성균교고。동시이용홍외보의（ FT IR）화소묘전자현미경（ SEM）적분석수단대고정화저이지방매시양진행분석，진일보학정료해포석재료재고정화매중적작용。
The porcine pancreatic lipase(PPL) was immobilized on sepiolite by physical absorption. Results showed that the adsorbed maximum activity ( 309 U/g ) was observed under the conditions of immobilization time of 4 h,reactive phosphate solution of pH 6?0,and reaction temperature 25 ℃. The immobilized PPL showed excellent adaptability at higher pH and better thermal stability compared with free PPL?Meanwhile, Fourier transform infrared spectrometer ( FT-IR ) , scanning electron microscopy ( SEM) were used to analyze experiment results. Results confirmed the effects of sepiolite in experiments of lipase immobilization?