CAJ | 학술논문

从烟梗中分离筛选得到1株能够对三苯甲烷类染料高效脱色的微生物，经ITS 5?8S rDNA分析鉴定为胶红酵母，命名为Rhodotorula mucilaginosa JB401。全波长扫描实验结果证实染料的脱色由胶红酵母降解结晶紫引起。为了提高R?mucilaginosa JB401脱色结晶紫的能力，通过单因素试验对R?mucilaginosa JB401的培养条件进行了优化，得出菌体生长24 h后以2％接种量接入初始pH为5的脱色培养基并在37℃摇床培养，可以取得最优脱色效果，此时脱色50、100和200 mg／L的结晶紫达到90％去除率分别需要3、6和14 h。此外，胶红酵母对温度和pH良好的适应性使其具有应用于工业废水处理的潜力。
종연경중분리사선득도1주능구대삼분갑완류염료고효탈색적미생물，경ITS 5?8S rDNA분석감정위효홍효모，명명위Rhodotorula mucilaginosa JB401。전파장소묘실험결과증실염료적탈색유효홍효모강해결정자인기。위료제고R?mucilaginosa JB401탈색결정자적능력，통과단인소시험대R?mucilaginosa JB401적배양조건진행료우화，득출균체생장24 h후이2％접충량접입초시pH위5적탈색배양기병재37℃요상배양，가이취득최우탈색효과，차시탈색50、100화200 mg／L적결정자체도90％거제솔분별수요3、6화14 h。차외，효홍효모대온도화pH량호적괄응성사기구유응용우공업폐수처리적잠력。
A strain efficiently decolorizing triphenylmethane dye was isolated from tobacco stem?By the analysis of ITS-5?8S rDNA,the strain was identified as Rhodotorula mucilaginosa JB401?The result of full wavelength scan confirmed that the delorization of crystal violet was caused by the dye degradation?In order to improve the the decolorization of crystal violet by R?mucilaginosa JB401,a single factor experiment was used to optimize the culture conditions of R?mucilaginosa JB401?The optimized decolorization conditions were 37℃,initial pH 5,2%inoculums under the shake-flask condition after cultivation for 24 h?Under the optimized culture conditions,50,100 and 200 mg/L of crystal violet were decolorized with 90%and time for decolorization were 3 h(50 mg/L),6 h(100 mg/L) and 14 h(200 mg/L), respectively?The excellent adaptability of R?mucilaginosa JB401 to pH and temperature exhibited potential applications.