CAJ | 학술논문

在浸没式膜反应器（ IMB）中利用恶臭假单胞菌ATCC 55687催化茚制备顺式茚二醇。采用25根膜丝制作的膜组件，在茚为3 g／L时，经过24 h培养，IMB中顺式茚二醇产量达到悬浮细胞反应器（ SCB）的4倍多；进一步，在培养开始阶段加入10 g／L sp 207树脂进行原位吸附，IMB中顺式茚二醇产量最高达709 mg／L，为SCB产量的660％，容积产率也从SCB中的6 mg／（ L·h）提高到30 mg／（ L·h）。在原位吸附IMB中，中空纤维膜既可作为固定化细胞载体，同时又可作为第二相吸附不溶性底物，降低底物和产物抑制，兼有固定化反应器和两相反应器的优点。
재침몰식막반응기（ IMB）중이용악취가단포균ATCC 55687최화인제비순식인이순。채용25근막사제작적막조건，재인위3 g／L시，경과24 h배양，IMB중순식인이순산량체도현부세포반응기（ SCB）적4배다；진일보，재배양개시계단가입10 g／L sp 207수지진행원위흡부，IMB중순식인이순산량최고체709 mg／L，위SCB산량적660％，용적산솔야종SCB중적6 mg／（ L·h）제고도30 mg／（ L·h）。재원위흡부IMB중，중공섬유막기가작위고정화세포재체，동시우가작위제이상흡부불용성저물，강저저물화산물억제，겸유고정화반응기화량상반응기적우점。
An immersed membrane bioreactor( IMB) with hollow fiber was fabricated for bioconversion of indene to cis-indandiol by Pseudomonas putida ATCC 55687?Compared with suspension culture bioreactor ( SCB) ,an increase of more than four-fold of cis-indandiol at 3 g/L indene concentration was achieved for 24 h in the IMB with a module of 25 membranes?The performance of the IMB was further improved by in situ adsorption and the highest product titer of 709 mg/L was achieved in the IMB with an addition of 10 g/L sp-207 resin at 0 h of fermentation,which was 660% of that in the SCB?The volumetric productivity of 30 mg/( L·h) was also higher than that of the 6 mg/( L·h) in the SCB?The hollow fiber membranes in the IMB served as a carrier to immobilize cells and a second phase to sequester insoluble reactant,thus reducing inhibition of substrate as well as products and facilitating a more effective biotransformation by combining with the advantages of the immobilized-cell bioreactor and the two-phase bioreactor.