CAJ | 학술논문

利用SSR标记研究50份糯玉米自交系的遗传变异，从40对核心引物中筛选出扩增条带清晰、稳定性和多态性好的18对引物，在50份糯玉米自交系中检测出87个等位基因变异，每对引物检测等位基因3～7个，平均4．83个；每对SSR引物的多态信息量（PIC ）为0.211～0.759，平均0．584。利用UPGMA聚类分析方法把50份供试糯玉米自交系分为4个类群，其划分结果与系谱分析基本一致。分别测定了50份糯玉米自交系子粒中蛋白质、淀粉、水分、脂肪和游离氨基酸的含量，结果表明：糯玉米子粒营养成分含量各不相同，游离氨基酸变异系数最大，淀粉变异系数最小，具有丰富的遗传多样性。基于50份自交系的品质性状数据，利用SPSS分析软件中平方欧式距离聚类分析方法进行聚类，结果划分为2个大类，4个亚类，类群之间具有较大差异。
이용SSR표기연구50빈나옥미자교계적유전변이，종40대핵심인물중사선출확증조대청석、은정성화다태성호적18대인물，재50빈나옥미자교계중검측출87개등위기인변이，매대인물검측등위기인3～7개，평균4．83개；매대SSR인물적다태신식량（PIC ）위0.211～0.759，평균0．584。이용UPGMA취류분석방법파50빈공시나옥미자교계분위4개류군，기화분결과여계보분석기본일치。분별측정료50빈나옥미자교계자립중단백질、정분、수분、지방화유리안기산적함량，결과표명：나옥미자립영양성분함량각불상동，유리안기산변이계수최대，정분변이계수최소，구유봉부적유전다양성。기우50빈자교계적품질성상수거，이용SPSS분석연건중평방구식거리취류분석방법진행취류，결과화분위2개대류，4개아류，류군지간구유교대차이。
The SSR markers were used to study the genetic variation of 50 waxy maize inbred lines ,and 18 pairs of primers were selected from 40 pairs of primers .A total of 87 polymorphic bands ,variation of 3~7 alleles for each primer ,and an average of 4.83 alleles per primer were detected ;polymorphic infor-mation content of each pair of SSR primers ranged from 0.211 to 0.759 averaged 0.584 .Using clustering analysis in UPGMA ,the waxy corn inbred lines were classified into 4 groups ,which was basically the same as germplasmic genealogy .The contents of protein ,starch ,moisture ,fat and free amino acids in the grain of waxy maize inbred lines were determined .The results showed that grain nutrient content of each waxy maize was not identical ,free amino acid variation coefficient was the maximum and that of the starch was the minimum ,indicating that the inbred lines were of rich genetic diversity .Based on the data of the quality traits in the 50 inbred lines ,adopting the squared Euclidean distance cluster analysis ,the 50 waxy maize inbred lines were divided into 2 groups ,4 subgroups ,and the there were significant differ-ences among the groups .