CAJ | 학술논문

目的合成并考察壳寡糖-聚乳酸嫁接物及其胶团的理化性质，荧光测定阿霉素胶团的载药量。方法采用纤维素酶对壳聚糖进行降解，制备低分子量壳聚糖、壳寡糖。以碳二亚胺为交联偶合剂合成壳寡糖-聚乳酸聚合物，超声分散法制备嫁接物胶团；微粒粒度及表面电位仪测定其粒径和表面电位；以阿霉素为模型药物，考察胶团对药物的增溶能力、药物包封率、载药量。结果在壳寡糖-聚乳酸嫁接物中，壳寡糖分子量为20000、聚乳酸分子量为5000的嫁接物平均粒径最小，药物包封率最高；聚乳酸分子量为5000壳寡糖-聚乳酸嫁接物载药胶团载药量较高。结论壳寡糖-聚乳酸聚合物胶团是一种良好的抗肿瘤药物载体，本方法可用于阿霉素胶团中阿霉素的含量测定。
목적합성병고찰각과당-취유산가접물급기효단적이화성질，형광측정아매소효단적재약량。방법채용섬유소매대각취당진행강해，제비저분자량각취당、각과당。이탄이아알위교련우합제합성각과당-취유산취합물，초성분산법제비가접물효단；미립립도급표면전위의측정기립경화표면전위；이아매소위모형약물，고찰효단대약물적증용능력、약물포봉솔、재약량。결과재각과당-취유산가접물중，각과당분자량위20000、취유산분자량위5000적가접물평균립경최소，약물포봉솔최고；취유산분자량위5000각과당-취유산가접물재약효단재약량교고。결론각과당-취유산취합물효단시일충량호적항종류약물재체，본방법가용우아매소효단중아매소적함량측정。
Objective To synthesize the chemical conjugate of polylactic acid-grafted chitosan oligosaccharide (CSO-PLA), and investigate the physicochemical properties of its polymeric micelles and its function as a drug carrier. the content of Adr of the micelles were determined by the fluorescence spectrometry method. Methods The chitosan oligosaccharide (CSO) was obtained by the degradation of chitosan in the presence of cellulase. The chemical conjugate of polylactic acid-grafted chitosan oligosaccharide was synthesized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodi mide. The micelle size, size distribution and zeta-potential were measured by light scat ering and electrophoretic mobility. Adriamycin as a model drug was then incorporated into the micelles, and the content of Adr of the micelles were determined. Results CSO-PLA with 20000 molecule weight of CSO and 5000 molecule weight of PLA possessed the smal est particle size, and the highest encapsulation ef iciency of the drug, and the loading capacity of CSO-PLA micelles with 5000 molecule weight of PLA were higher. CSO-PLA micelles with 5000 molecule weight of PLA displayed significant sustained-release characteristic. Conclusion The CSO-PLA micelles could use as the carrier for water-insoluble anti-tumor drug. The method is reliablefor the dertermination of Adr in the micelles of Adr.