CAJ | 학술논문

研究添加复合微生态制剂和饲用抗生素对肉鸡血清生化指标和肠道酶活性的影响。试验选取500只1日龄雌性健康AA肉鸡随机分为5组,每组4个重复,每个重复25只。试验预试期7d,Ⅰ组为对照组,饲喂不含抗生素的基础饲粮,Ⅴ组为饲用抗生素组,Ⅱ、Ⅲ、Ⅳ组为微生态制剂组,添加量分别为1.0‰、2.0‰、4.0‰。正试期为35d。结果表明,除42日龄外,其余各试验阶段Ⅲ组肉鸡血清总蛋白含量均显著高于对照组和抗生素组（P〈0.05）;除14日龄外,Ⅲ组肉鸡血清尿素氮含量均显著低于对照组和抗生素组（P〈0.05）;在每个试验阶段,Ⅲ组回肠内容物蛋白酶活性均显著高于对照组和抗生素组（P〈0.05）,且除14日龄外,Ⅲ组肉鸡空肠内容物蛋白酶活性最高,显著高于对照组和抗生素组（P〈0.05）;除14日龄外,Ⅲ组空肠和回肠内容物淀粉酶活性均最高,显著高于对照组和抗生素组（P〈0.05）。由此可知,2.0‰复合微生态制剂能够显著提高肉鸡血清总蛋白水平,增强空肠和回肠内容物蛋白酶和淀粉酶活性,同时在一定程度上降低了肉鸡血清尿素氮含量。
연구첨가복합미생태제제화사용항생소대육계혈청생화지표화장도매활성적영향。시험선취500지1일령자성건강AA육계수궤분위5조,매조4개중복,매개중복25지。시험예시기7d,Ⅰ조위대조조,사위불함항생소적기출사량,Ⅴ조위사용항생소조,Ⅱ、Ⅲ、Ⅳ조위미생태제제조,첨가량분별위1.0‰、2.0‰、4.0‰。정시기위35d。결과표명,제42일령외,기여각시험계단Ⅲ조육계혈청총단백함량균현저고우대조조화항생소조（P〈0.05）;제14일령외,Ⅲ조육계혈청뇨소담함량균현저저우대조조화항생소조（P〈0.05）;재매개시험계단,Ⅲ조회장내용물단백매활성균현저고우대조조화항생소조（P〈0.05）,차제14일령외,Ⅲ조육계공장내용물단백매활성최고,현저고우대조조화항생소조（P〈0.05）;제14일령외,Ⅲ조공장화회장내용물정분매활성균최고,현저고우대조조화항생소조（P〈0.05）。유차가지,2.0‰복합미생태제제능구현저제고육계혈청총단백수평,증강공장화회장내용물단백매화정분매활성,동시재일정정도상강저료육계혈청뇨소담함량。
The effects of adding compound micro-ecological preparation and feed antibiotics on the serum biochemical indices and intestinal enzyme activity of broilers were studied. Five hundred one-day-old female AA broiler chicken were randomly divided into five groups with 4 repetitions in each group and 25 chickens per repetition. The pre-trail period of the experiment was 7 days. The chicken in the control group（groupⅠ）were only fed with the basal diet（no antibiotics）,chicken in groupⅤwas fed with antibiotics and chicken in group Ⅱ,Ⅲ and Ⅳ were fed with feeding the diet with adding 1.0‰,2.0‰ and 4.0‰ micro-ecological preparation. The test period lasted for 35 days. The results showed that the content of serum total protein in group Ⅲ in each test stages was significantly higher than that in control group and antibiotics group（P0.05）, except 42-day-old. Except 14- day-old, the content of serum urea nitrogen in group Ⅲ of broilers was significantly lower than that in control group and antibiotics group （P0.05）. In all test stages, the activity of protease in ileum contents in group Ⅲ was significantly higher than that in control group and antibiotics group （P0.05）. And the activity of protease in jejunum contents in group Ⅲ was significantly higher than that in control group and antibiotics group （P0.05）, except 14-day-old. Except 14-day-old, the activity of amylase in ileum and jejunum contents in group Ⅲ was significantly higher than that in control group and antibiotics group（P0.05）. Therefore, 2.0‰ compound micro-ecological preparation could significantly increase the level of total protein in the serum of broilers, enhance the activities of protease and amylase in ileum and jejunum contents and decrease the content of serum urea nitrogen of broilers to some degree.