农药科学与管理
農藥科學與管理
농약과학여관리
PESTICIDE SCIENCE AND ADMINISTRATION
2012年
4期
31-34
,共4页
咯菌腈%溴虫腈%虱螨脲%液相色谱%残留
咯菌腈%溴蟲腈%虱螨脲%液相色譜%殘留
각균정%추충정%슬만뇨%액상색보%잔류
fludioxonil%lufenuron%chlorfenapyr%HPLC%residue
建立了同时测定蔬菜（青菜、菜豆、卷心菜、番茄）中咯菌腈、虱螨脲和溴虫腈等农药残留的高效液相色谱法。蔬菜以乙腈高速匀浆提取,过NH2小柱净化,再用甲醇定容。而土壤样品较干净,经乙腈振荡提取1h,浓缩后定容过膜即可。采用甲醇-水为流动相,利用C18柱和紫外检测器（检测波长：260nm）对待测组分进行了分离和测定。实验证明,添加浓度在0.1、0.5、1mg/kg时,蔬菜中咯菌腈、虱螨脲和溴虫腈平均添加回收率在78.2～117.3%之间,相对标准偏差（RSD）〈10%,咯菌腈、虱螨脲和溴虫腈在样品中的最低检出浓度为0.1mg/kg,最小检出量为：1ng。
建立瞭同時測定蔬菜（青菜、菜豆、捲心菜、番茄）中咯菌腈、虱螨脲和溴蟲腈等農藥殘留的高效液相色譜法。蔬菜以乙腈高速勻漿提取,過NH2小柱淨化,再用甲醇定容。而土壤樣品較榦淨,經乙腈振盪提取1h,濃縮後定容過膜即可。採用甲醇-水為流動相,利用C18柱和紫外檢測器（檢測波長：260nm）對待測組分進行瞭分離和測定。實驗證明,添加濃度在0.1、0.5、1mg/kg時,蔬菜中咯菌腈、虱螨脲和溴蟲腈平均添加迴收率在78.2～117.3%之間,相對標準偏差（RSD）〈10%,咯菌腈、虱螨脲和溴蟲腈在樣品中的最低檢齣濃度為0.1mg/kg,最小檢齣量為：1ng。
건립료동시측정소채（청채、채두、권심채、번가）중각균정、슬만뇨화추충정등농약잔류적고효액상색보법。소채이을정고속균장제취,과NH2소주정화,재용갑순정용。이토양양품교간정,경을정진탕제취1h,농축후정용과막즉가。채용갑순-수위류동상,이용C18주화자외검측기（검측파장：260nm）대대측조분진행료분리화측정。실험증명,첨가농도재0.1、0.5、1mg/kg시,소채중각균정、슬만뇨화추충정평균첨가회수솔재78.2～117.3%지간,상대표준편차（RSD）〈10%,각균정、슬만뇨화추충정재양품중적최저검출농도위0.1mg/kg,최소검출량위：1ng。
A high-performance liquid chromatography （HPLC） method was developed for the determination of Fludioxonil, Lufenuron and Chlorfenapyr in vegetables （green grocery, cowpea, cabbage and tomato） . The residues of vegetable samples were extracted with acetonitrile, and vegetable samples were cleaned up with NH2 Solid-phase extraction , and condensed to dry. Determinations of analytes were completed using HPLC with DAD detection at 26Onto. Under HPLC conditions, The fortified recoveries of Fludioxonil, Lufenuron and Chlorfenapyr in vegetable samples were in range of 78.2-117.3%, with relative standard deviations less than 10%. and the minimum determination concentration was 0.02 mg/kg in these samples, the detectable limit of three pesticides was estimated to be lng.
