CAJ | 학술논문

通过对棕色棉BC05叶片进行Kan浓度梯度涂抹实验,确定了Kan涂抹筛选的最佳浓度为3.0 g/L,最佳观察时间为涂抹第5天。对Kan抗性植株进行PCR鉴定表明,Kan涂抹筛选可基本准确的鉴别出转基因材料与非转基因材料,但目标基因是否成功整合还必须进行PCR鉴定,田间卡那霉素筛选结合PCR分子鉴定是转基因棉花后代大规模群体筛选的有效方法。
통과대종색면BC05협편진행Kan농도제도도말실험,학정료Kan도말사선적최가농도위3.0 g/L,최가관찰시간위도말제5천。대Kan항성식주진행PCR감정표명,Kan도말사선가기본준학적감별출전기인재료여비전기인재료,단목표기인시부성공정합환필수진행PCR감정,전간잡나매소사선결합PCR분자감정시전기인면화후대대규모군체사선적유효방법。
The smear test of Kan concentration gradient was conducted in brown cotton BC05 leaves,then the best screening concentration of 3.0 g/L and the best observation time of fifth day were obtained.Through the PCR identification in Kan-resistant plants,the results showed that the method was able to identify whether plant is genetically modified material or non-GM material.But whether the target gene was successfully integrated was remain to be identified by PCR.Therefore,the kanamycin screening combining with PCR molecular identification is an effective method to screened large groups of genetically modified cotton offspring.