CAJ | 학술논문

采用高效液相色谱法测定人参中8种主要单体皂苷的含量。使用Agilent ZORBAX -C18（250mm ×4.6mm ，5μm ）色谱柱，流动相乙腈-水梯度洗脱流速1.0mL/min；检测波长203nm；柱温25℃；进样量20μL。在本色谱条件下，8个人参皂苷类成分之间有良好的分离度，各成分的浓度和相应的峰面积之间呈现良好的线性关系（ r＝0.9999，n＝7），精密度、重复性及加样回收率的RSD均小于3％。本实验可同时测定8种人参皂苷类成分的含量，不同地区人参单体皂苷含量比较结果表明：3个产地人参中8种单体皂苷含量，靖宇县＞集安市＞珲春市，说明靖宇县人参皂苷含量较高，质量较优；而珲春市较之稍差，但亦明显高于国家药典要求。
채용고효액상색보법측정인삼중8충주요단체조감적함량。사용Agilent ZORBAX -C18（250mm ×4.6mm ，5μm ）색보주，류동상을정-수제도세탈류속1.0mL/min；검측파장203nm；주온25℃；진양량20μL。재본색보조건하，8개인삼조감류성분지간유량호적분리도，각성분적농도화상응적봉면적지간정현량호적선성관계（ r＝0.9999，n＝7），정밀도、중복성급가양회수솔적RSD균소우3％。본실험가동시측정8충인삼조감류성분적함량，불동지구인삼단체조감함량비교결과표명：3개산지인삼중8충단체조감함량，정우현＞집안시＞혼춘시，설명정우현인삼조감함량교고，질량교우；이혼춘시교지초차，단역명현고우국가약전요구。
Eight kinds of monomer saponin contents of Panax ginseng from different regions were determined by high performance liquid chro-matography(HPLC) .Chromatographic conditions :chromatographic column:Agilent ZORBAX -C18 (250mm × 4.6mm ,5μm ) column;mobile phase:acetonitrile -water gradientelution ;flow rate:1.0mL/min;detection wavelength :203nm;column temperature :25℃ ;injection volume :20μL .Results showed:that etight kinds of ginsenoside had a good separation in the chromatographic conditions ,and it showed a good linear re-lationship between individual component concentration and peak area (r=0.999 9 ,n=7) .The precision ,repeatability and the recovery of the RSD were less than 3% .So eight kinds of ginsenoside contents could be determined simultaneously in this conditions .The eight kinds of gin-senoside contents from three different regions were the order of Jingyu >Ji'an>Hunchun ,so the ginsenoside contents from Jingyu was the high-est and the content from Hunchun was the lowest ,but the ginsenoside contents from three different regions were higher than the standard validat-ed by pharmacopoeia of the people's republic of China .