CAJ | 학술논문

以健康饱满成熟的三七种子为外植体，接种到不同处理的培养基上，诱导实生苗。结果表明，剥除三七种皮，用70％的乙醇浸泡1min ，再用0.1％升汞消毒灭菌10min ，无菌水冲洗4～5次，接种到MS＋2，4-D （1.0mg/L ）＋KT （0.5mg/L ）＋GA3（40mg/L ）＋蔗糖（30g/L ）＋琼脂粉（7g/L ）的培养基（pH 5.8）中，根、茎、叶生长表现良好，从而初步建立了三七实生苗组培体系。
이건강포만성숙적삼칠충자위외식체，접충도불동처리적배양기상，유도실생묘。결과표명，박제삼칠충피，용70％적을순침포1min ，재용0.1％승홍소독멸균10min ，무균수충세4～5차，접충도MS＋2，4-D （1.0mg/L ）＋KT （0.5mg/L ）＋GA3（40mg/L ）＋자당（30g/L ）＋경지분（7g/L ）적배양기（pH 5.8）중，근、경、협생장표현량호，종이초보건립료삼칠실생묘조배체계。
Plantlet of Panax notoginseng induced from health full mature seeds which were inoculated on the different medium for establishment of tissue culture system and induction of seedling .The results showed that peeling seeds which were quick dip in 0.1% mercuric chloride for 10 minutes after sterilization in 70% alcohol for 1 minute were incubated in Petri dishes containing MS medium with 1.0mg/L 2 ,4-D ,0.5mg/L KT ,40mg/L GA3 ,30g/L sucrose and 7.0g/L gelrite under pH 5.8 .And the seedling of notoginseng were growing well .A preliminary efficient system for inducing seedling in Panax notoginseng was established .