CAJ | 학술논문

目的：观察地下停车场汽车尾气污染物对小鼠免疫系统的影响。方法将30只雌性昆明系小鼠按随机数字表法分为对照组和污染组，每组15只。对照组饲养于中山大学南校区力学楼顶层相对清洁区，污染组饲养于中山大学附属第一医院地下停车场负二层。饲养期间记录两处饲养地点大气污染物水平，并观察两组小鼠进食量和体质量等一般指标。饲养3个月后观察小鼠的存活情况，用酶联免疫吸附试验（ELISA）测定小鼠外周血清肿瘤坏死因子-α（TNF-α）、转化生长因子-β1（TGF-β1）和白细胞介素-4（IL-4）含量。结果污染组饲养点空气中污染物一氧化碳（CO，mg/m3：16.784±3.093比2.249±0.112）、氮氧化物（NOx， mg/m3：0.318±0.041比0.065±0.030）、空气中可悬浮颗粒物（PM2.5，mg/m3：0.309±0.051比0.055±0.013）水平均明显高于对照组（均P＜0.001），污染组小鼠总进食量（670 g比960 g）及体质量〔（37.13±1.11）g比（41.23±1.34）g〕明显低于对照组（P＜0.001）；两组均无死亡。污染组小鼠血清TNF-α（ng/L：247.93±22.25比143.33±39.01），TFG-β1（ng/L：395.77±41.29比319.15±20.72），IL-4（ng/L：231.89±20.04比194.09±3.57）均明显高于对照组（均P＜0.001）。结论地下停车场汽车尾气污染物对小鼠血清炎症因子存在明显影响，提示污染物存在可以激活机体炎症反应过程，导致机体免疫功能紊乱。
목적：관찰지하정차장기차미기오염물대소서면역계통적영향。방법장30지자성곤명계소서안수궤수자표법분위대조조화오염조，매조15지。대조조사양우중산대학남교구역학루정층상대청길구，오염조사양우중산대학부속제일의원지하정차장부이층。사양기간기록량처사양지점대기오염물수평，병관찰량조소서진식량화체질량등일반지표。사양3개월후관찰소서적존활정황，용매련면역흡부시험（ELISA）측정소서외주혈청종류배사인자-α（TNF-α）、전화생장인자-β1（TGF-β1）화백세포개소-4（IL-4）함량。결과오염조사양점공기중오염물일양화탄（CO，mg/m3：16.784±3.093비2.249±0.112）、담양화물（NOx， mg/m3：0.318±0.041비0.065±0.030）、공기중가현부과립물（PM2.5，mg/m3：0.309±0.051비0.055±0.013）수평균명현고우대조조（균P＜0.001），오염조소서총진식량（670 g비960 g）급체질량〔（37.13±1.11）g비（41.23±1.34）g〕명현저우대조조（P＜0.001）；량조균무사망。오염조소서혈청TNF-α（ng/L：247.93±22.25비143.33±39.01），TFG-β1（ng/L：395.77±41.29비319.15±20.72），IL-4（ng/L：231.89±20.04비194.09±3.57）균명현고우대조조（균P＜0.001）。결론지하정차장기차미기오염물대소서혈청염증인자존재명현영향，제시오염물존재가이격활궤체염증반응과정，도치궤체면역공능문란。
Objective To observe the effect of automobile exhaust pollutants in air of underground parking area on immune system of mice. Methods Thirty female Kunming mice were divided into a control(relatively clean) group and a polluted group(each n=15)by a random number table method. The control group was housed on the roof of a mechanical building located at the south campus of Sun Yat-sen University,while the polluted group was housed in the minus second layer of underground parking lot at the First Affiliated Hospital of Sun Yat-sen University. The data of atmospheric pollutants were recorded at both feeding sites. The mice's food-intake and body weight were also observed. After 3 months of observation,the mice were sacrificed and peripheral serum was harvested. Enzyme-linked immunosorbent assay(ELISA)was performed to detect the level of tumor necrotic factor-α(TNF-α),transforming growth factor-β1(TGF-β1)and interleukin-4(IL-4). Results The levels of air pollutants in pollution group were higher than those in control group〔carbonmonoxide(CO,mg/m3:16.784±3.093 vs. 2.249±0.112),nitrogen oxides(NOx, mg/m3:0.318±0.041 vs. 0.065±0.030)and particulate matter(PM2.5,mg/m3:0.309±0.051 vs. 0.055±0.013),all P<0.001〕. The amount of food intake and body weight of the polluted group were obviously lower than those of the control group〔food-intake:670 g vs. 960 g,body weight:(37.13±1.11)g vs.(41.23±1.34)g, both P<0.001〕. There was no abnormal death in both groups. The serum levels of TNF-α(ng/L:247.93±22.25 vs. 143.33±39.01), TFG-β1(ng/L:395.77±41.29 vs. 319.15±20.72) and IL-4(ng/L:231.89±20.04 vs. 194.09±3.57)were significantly higher in the mice of polluted group than those in the control group(all P<0.001). Conclusions The automobile exhaust pollutants in the air of underground parking lot have obvious effects on the levels of serum inflammatory cytokines of mice. It is suggested that the presence of pollutants activate the body's inflammatory process and lead to disturbance of immune system of female Kunming mice.