实用肝脏病杂志
實用肝髒病雜誌
실용간장병잡지
JOURNAL OF CLINICAL HEPATOLOGY
2013年
6期
502-504
,共3页
邵小娟%张艳梅%魏艳玲%樊丽琳%刘卉%杨均%邢寒阳%崔红莉%颜綦先%唐雯%孙文静%陈东风
邵小娟%張豔梅%魏豔玲%樊麗琳%劉卉%楊均%邢寒暘%崔紅莉%顏綦先%唐雯%孫文靜%陳東風
소소연%장염매%위염령%번려림%류훼%양균%형한양%최홍리%안기선%당문%손문정%진동풍
非酒精性脂肪性肝炎%巨噬细胞%M1亚群%M2亚群
非酒精性脂肪性肝炎%巨噬細胞%M1亞群%M2亞群
비주정성지방성간염%거서세포%M1아군%M2아군
Nonalcoholic steatohepatitis%Macrophages%Macrophage polarization
目的：研究非酒精性脂肪性肝炎（NASH）患者网膜脂肪及皮下脂肪巨噬细胞数量的变化。方法选择NASH伴单纯性胆囊结石患者22例和单纯性胆囊结石患者28例。在腹腔镜手术中取肝组织、网膜脂肪组织和皮下脂肪组织。常规检测BMI、腹围、血糖、血酯、HOMA指数、胰岛素、游离脂肪酸；采用免疫组化法检测脂肪组织巨噬细胞。以抗CD68抗体标记巨噬细胞，抗CD11C标记M1亚群巨噬细胞，抗CD206抗体标记M2亚群巨噬细胞。结果 NASH患者血清AST （31.43±14.76）U/L对（23.68±9.64）U/L、TG （2.42±0.82）mmol/L对（1.65±0.47） mmol/L、胰岛素（83.18±44.34）mlu/L对（51.15±32.12）mlu/L和HOMA指数（21.00±14.75）对（12.43±10.66）显著高于胆囊结石患者（P<0.05）；NASH患者网膜脂肪组织巨噬细胞数量为（34.41±15.35），皮下脂肪组织巨噬细胞数量为（27.91±11.13），而胆囊结石患者分别为（29.93±11.49）和（15.57±5.67），（P<0.01）；在22例NASH患者中，其NAS评分7分2例，6分6例，5分9例，4分5例。NASH患者皮下脂肪组织巨噬细胞、M1亚群巨噬细胞和M2亚群巨噬细胞数量与NAS评分呈正相关（P<0.01），但在网膜脂肪组织中三种细胞与NAS评分无显著性相关（P>0.05）。结论网膜脂肪及皮下脂肪中的巨噬细胞数量变化参与了NASH的发病过程。
目的：研究非酒精性脂肪性肝炎（NASH）患者網膜脂肪及皮下脂肪巨噬細胞數量的變化。方法選擇NASH伴單純性膽囊結石患者22例和單純性膽囊結石患者28例。在腹腔鏡手術中取肝組織、網膜脂肪組織和皮下脂肪組織。常規檢測BMI、腹圍、血糖、血酯、HOMA指數、胰島素、遊離脂肪痠；採用免疫組化法檢測脂肪組織巨噬細胞。以抗CD68抗體標記巨噬細胞，抗CD11C標記M1亞群巨噬細胞，抗CD206抗體標記M2亞群巨噬細胞。結果 NASH患者血清AST （31.43±14.76）U/L對（23.68±9.64）U/L、TG （2.42±0.82）mmol/L對（1.65±0.47） mmol/L、胰島素（83.18±44.34）mlu/L對（51.15±32.12）mlu/L和HOMA指數（21.00±14.75）對（12.43±10.66）顯著高于膽囊結石患者（P<0.05）；NASH患者網膜脂肪組織巨噬細胞數量為（34.41±15.35），皮下脂肪組織巨噬細胞數量為（27.91±11.13），而膽囊結石患者分彆為（29.93±11.49）和（15.57±5.67），（P<0.01）；在22例NASH患者中，其NAS評分7分2例，6分6例，5分9例，4分5例。NASH患者皮下脂肪組織巨噬細胞、M1亞群巨噬細胞和M2亞群巨噬細胞數量與NAS評分呈正相關（P<0.01），但在網膜脂肪組織中三種細胞與NAS評分無顯著性相關（P>0.05）。結論網膜脂肪及皮下脂肪中的巨噬細胞數量變化參與瞭NASH的髮病過程。
목적：연구비주정성지방성간염（NASH）환자망막지방급피하지방거서세포수량적변화。방법선택NASH반단순성담낭결석환자22례화단순성담낭결석환자28례。재복강경수술중취간조직、망막지방조직화피하지방조직。상규검측BMI、복위、혈당、혈지、HOMA지수、이도소、유리지방산；채용면역조화법검측지방조직거서세포。이항CD68항체표기거서세포，항CD11C표기M1아군거서세포，항CD206항체표기M2아군거서세포。결과 NASH환자혈청AST （31.43±14.76）U/L대（23.68±9.64）U/L、TG （2.42±0.82）mmol/L대（1.65±0.47） mmol/L、이도소（83.18±44.34）mlu/L대（51.15±32.12）mlu/L화HOMA지수（21.00±14.75）대（12.43±10.66）현저고우담낭결석환자（P<0.05）；NASH환자망막지방조직거서세포수량위（34.41±15.35），피하지방조직거서세포수량위（27.91±11.13），이담낭결석환자분별위（29.93±11.49）화（15.57±5.67），（P<0.01）；재22례NASH환자중，기NAS평분7분2례，6분6례，5분9례，4분5례。NASH환자피하지방조직거서세포、M1아군거서세포화M2아군거서세포수량여NAS평분정정상관（P<0.01），단재망막지방조직중삼충세포여NAS평분무현저성상관（P>0.05）。결론망막지방급피하지방중적거서세포수량변화삼여료NASH적발병과정。
Objective To investigate the changes of macrophage counts in adipose tissues in patients with nonalcoholic steatohepatitis(NASH). Method 22 patients with NASH and 28 with gallbladder stone were includ-ed in this study. The epiploic adipose tissues and subcutaneous adipose tissues during laparoscopic cholecystecto-my were obtained. We detected the clinical data as respect to age,gender,BMI,abdominal circumference,glucose, ALT,AST,total cholesterol (TG),HOMA index,insulin and free fatty acids. The infiltration of macrophages (anti-CD68),macrophage M1(anti-CD11C) and macrophage M2(anti-CD206)in epiploic and subcutaneous adipose tis-sues were assessed by immunohistochemical staining. Results The serum AST(31.43±14.76)U/L vs. (23.68± 9.64)u/L,TG(2.42±0.82)mmol/L vs.(1.65±0.47)mmol/L,insulin(83.18±44.34)mlu/Lvs. (51.15±32.12)mlu/L and HOMA-index(21.00±14.75) vs.(12.43±10.66)in patients with NASH were significantly higher than those in pa-tients with gallbladder stone(P<0.05);The counts of macrophages in epiploic and in subcutaneous adipose tissues (34.41±15.35)and(27.91±11.13),respectively in patients with NASH were higher than those(29.93±11.49)and (15.57±5.67),respectively,P<0.01) in patients with gallbladder stone;The NAS scores were 7 in two,6 in six,5 in nine,and 4 in five cases out of the 22 patients with NASH;The counts of macrophages,M1 and M2 macrophages in subcutaneous adipose tissues in NASH patients were positively correlated to NAS scores(P<0.01), but not in epiploic adipose tissues(P>0.05). Conclusion The infiltration of the macrophages and macrophage po-larization in epiploic and subcutaneous adipose tissues were associated with the development of NASH.
