山东理工大学学报（自然科学版）
山東理工大學學報（自然科學版）
산동리공대학학보（자연과학판）
JOURNAL OF SHANDONG UNIVERSITY OF TECHNOLOGY(SCIENCE AND TECHNOLOGY)
2013年
6期
26-31
,共6页
嗜热链球菌%响应曲面法%生长条件%优化
嗜熱鏈毬菌%響應麯麵法%生長條件%優化
기열련구균%향응곡면법%생장조건%우화
S .thermophilus%growth condition%response surface methodology%optimization
采用单因素试验和响应曲面法对酸奶中嗜热链球菌 H1的最适生长条件进行了研究和优化．根据回归分析得到的最适培养条件为：温度41．02℃，pH5．74，接种量0．31mL ·50mL -1，盐浓度0．23％，此时菌株的光密度OD600为1．176．经实验验证，在该条件下菌株光密度OD600为1．162，其相对误差小于2％．
採用單因素試驗和響應麯麵法對痠奶中嗜熱鏈毬菌 H1的最適生長條件進行瞭研究和優化．根據迴歸分析得到的最適培養條件為：溫度41．02℃，pH5．74，接種量0．31mL ·50mL -1，鹽濃度0．23％，此時菌株的光密度OD600為1．176．經實驗驗證，在該條件下菌株光密度OD600為1．162，其相對誤差小于2％．
채용단인소시험화향응곡면법대산내중기열련구균 H1적최괄생장조건진행료연구화우화．근거회귀분석득도적최괄배양조건위：온도41．02℃，pH5．74，접충량0．31mL ·50mL -1，염농도0．23％，차시균주적광밀도OD600위1．176．경실험험증，재해조건하균주광밀도OD600위1．162，기상대오차소우2％．
The growth conditions of S .thermophilus H1 were studied by single factor experiment and response surface methodology . The results showed that the optimal parameters for the growth conditions as follows :the temperature was 41 .02℃ ,the pH was value 5 .74 ,the inoculum size was 0 .31mL · 50mL -1 ,the salt concentration was 0 .23% ,and the OD600 of strain HI was 1 . 176 .Under the optimal conditions the OD600 of strain HI was 1 .162 ,The relative error was bel-lo w 2% .
