草原与草坪
草原與草坪
초원여초평
GRASSLAND AND TURF
2013年
6期
1-8
,共8页
陈力玉%张淑卿%李剑峰%师尚礼%霍平慧%苗阳阳
陳力玉%張淑卿%李劍峰%師尚禮%霍平慧%苗暘暘
진력옥%장숙경%리검봉%사상례%곽평혜%묘양양
根瘤菌%接种%苜蓿幼苗%生物量%固氮酶活性
根瘤菌%接種%苜蓿幼苗%生物量%固氮酶活性
근류균%접충%목숙유묘%생물량%고담매활성
rhizobia%inoculation%alfalfa plant%biomass%nitrogenase activity
将含有 cfp(cyan fluorescent protein )基因质粒的荧光标记根瘤菌 S.LZgn5-cfp6、S.LZgn5-cfp16、S.LZgn5-cfp28,S.12531-cfp2、S.12531-cfp13、S.12531-cfp26、S.LH3436-cfp1、S.LH3436-cfp2、S.LH3436-cfp6接种于甘农5号紫花苜蓿,测定苜蓿幼苗生物量、单株结瘤数、固氮酶活性等,结果表明：荧光标记根瘤菌可有效促进植株生长和生物量的积累,有明显促生效果；与出发菌相比,荧光标记根瘤菌未对苜蓿幼苗产生显著的不良影响,能在植物体内稳定表达,具有与出发菌株相近的促生能力。
將含有 cfp(cyan fluorescent protein )基因質粒的熒光標記根瘤菌 S.LZgn5-cfp6、S.LZgn5-cfp16、S.LZgn5-cfp28,S.12531-cfp2、S.12531-cfp13、S.12531-cfp26、S.LH3436-cfp1、S.LH3436-cfp2、S.LH3436-cfp6接種于甘農5號紫花苜蓿,測定苜蓿幼苗生物量、單株結瘤數、固氮酶活性等,結果錶明：熒光標記根瘤菌可有效促進植株生長和生物量的積纍,有明顯促生效果；與齣髮菌相比,熒光標記根瘤菌未對苜蓿幼苗產生顯著的不良影響,能在植物體內穩定錶達,具有與齣髮菌株相近的促生能力。
장함유 cfp(cyan fluorescent protein )기인질립적형광표기근류균 S.LZgn5-cfp6、S.LZgn5-cfp16、S.LZgn5-cfp28,S.12531-cfp2、S.12531-cfp13、S.12531-cfp26、S.LH3436-cfp1、S.LH3436-cfp2、S.LH3436-cfp6접충우감농5호자화목숙,측정목숙유묘생물량、단주결류수、고담매활성등,결과표명：형광표기근류균가유효촉진식주생장화생물량적적루,유명현촉생효과；여출발균상비,형광표기근류균미대목숙유묘산생현저적불량영향,능재식물체내은정표체,구유여출발균주상근적촉생능력。
Fluorescence marked rhizobias (S.LZgn5-cfp6,S.LZgn5-cfp1 6,S.LZgn5-cfp28,S.12531-cfp2,S. 12531-cfp13,S.12531-cfp26,S.LH3436-cfp1,S.LH3436-cfp2,S.LH3436-cfp6)that contained the plasmid with cfp (cyan fluorescent protein)gene were inoculated to Gannong No.5 alfalfa.Biomass,nodule number,nitroge-nase activity and so on were measured.The result showed that the fluorescence marked rhizobias could promote plant growth and biomass accumulation of inoculated alfalfa.Compared with their corresponding original rhizo-bias,the fluorescence marked rhizobias did no harm to alfalfa seedling,and could express stably in alfalfa plant, and had the similar growth promotion ability with original rhizobias.
