癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2013年
6期
426-429,434
,共5页
林桂淼%梅树江%林苏霞%许改霞%李慧%朱玥荃%林晓潭%蔡志明%王晓梅%马汉武%李媛%路滟%秦彦珉%易婉娴
林桂淼%梅樹江%林囌霞%許改霞%李慧%硃玥荃%林曉潭%蔡誌明%王曉梅%馬漢武%李媛%路滟%秦彥珉%易婉嫻
림계묘%매수강%림소하%허개하%리혜%주모전%림효담%채지명%왕효매%마한무%리원%로염%진언민%역완한
腔前卵泡%三维培养%卵子成熟%静磁场
腔前卵泡%三維培養%卵子成熟%靜磁場
강전란포%삼유배양%란자성숙%정자장
preantral follicle%3D culture%oocyte maturation%static magnetic field
目的：用三维小鼠腔前卵泡体外培养成熟体系检测静磁场对生殖细胞发育成熟的影响。方法：从12日龄昆明小鼠的卵巢中，机械分离出直径为(130±10)μm的腔前卵泡，用海藻酸钠凝胶将单个卵泡包埋后，放入预平衡的培养液滴中培养，并随机分成对照组、5和450 mT暴磁组，隔天半量换液，在倒置显微镜下测量其直径，连续培养8 d，对成腔的卵泡进行体外人绒毛膜促性腺激素(HCG)刺激16~18 h后，检测卵母细胞成熟情况。结果：各组卵泡形态学发育均良好，随着体外培养时间的延长，卵泡逐渐增大，在第4天450 mT暴磁组卵泡直径与对照组相比增大显著(<0.05)，第6天5和450 mT暴磁组均明显高于对照组(P<0.05)。随着磁场强度增强，卵泡成腔率亦比对照组显著提高(P<0.05)。而5 mT暴磁组成熟率与对照组相比无明显变化(P>0.05)，但450 mT组与对照组相比显著降低(P<0.05)。结论：海藻酸盐凝胶可维持昆明小鼠腔前卵泡体外正常生长发育。随着磁场强度增强，卵泡的成腔率显著性提高，成腔卵泡直径显著增大，但高强度的静磁场可能会影响卵母细胞的成熟过程。P
目的：用三維小鼠腔前卵泡體外培養成熟體繫檢測靜磁場對生殖細胞髮育成熟的影響。方法：從12日齡昆明小鼠的卵巢中，機械分離齣直徑為(130±10)μm的腔前卵泡，用海藻痠鈉凝膠將單箇卵泡包埋後，放入預平衡的培養液滴中培養，併隨機分成對照組、5和450 mT暴磁組，隔天半量換液，在倒置顯微鏡下測量其直徑，連續培養8 d，對成腔的卵泡進行體外人絨毛膜促性腺激素(HCG)刺激16~18 h後，檢測卵母細胞成熟情況。結果：各組卵泡形態學髮育均良好，隨著體外培養時間的延長，卵泡逐漸增大，在第4天450 mT暴磁組卵泡直徑與對照組相比增大顯著(<0.05)，第6天5和450 mT暴磁組均明顯高于對照組(P<0.05)。隨著磁場彊度增彊，卵泡成腔率亦比對照組顯著提高(P<0.05)。而5 mT暴磁組成熟率與對照組相比無明顯變化(P>0.05)，但450 mT組與對照組相比顯著降低(P<0.05)。結論：海藻痠鹽凝膠可維持昆明小鼠腔前卵泡體外正常生長髮育。隨著磁場彊度增彊，卵泡的成腔率顯著性提高，成腔卵泡直徑顯著增大，但高彊度的靜磁場可能會影響卵母細胞的成熟過程。P
목적：용삼유소서강전란포체외배양성숙체계검측정자장대생식세포발육성숙적영향。방법：종12일령곤명소서적란소중，궤계분리출직경위(130±10)μm적강전란포，용해조산납응효장단개란포포매후，방입예평형적배양액적중배양，병수궤분성대조조、5화450 mT폭자조，격천반량환액，재도치현미경하측량기직경，련속배양8 d，대성강적란포진행체외인융모막촉성선격소(HCG)자격16~18 h후，검측란모세포성숙정황。결과：각조란포형태학발육균량호，수착체외배양시간적연장，란포축점증대，재제4천450 mT폭자조란포직경여대조조상비증대현저(<0.05)，제6천5화450 mT폭자조균명현고우대조조(P<0.05)。수착자장강도증강，란포성강솔역비대조조현저제고(P<0.05)。이5 mT폭자조성숙솔여대조조상비무명현변화(P>0.05)，단450 mT조여대조조상비현저강저(P<0.05)。결론：해조산염응효가유지곤명소서강전란포체외정상생장발육。수착자장강도증강，란포적성강솔현저성제고，성강란포직경현저증대，단고강도적정자장가능회영향란모세포적성숙과정。P
OBJECTIVE: The reproductive toxicity of static magnetic field (SMF) was investigated using mouse preantral follicle 3D culture in vitro system established in our laboratory. METHODS:Ovaries was removed from 12 days old female mice. (130±10)μm preantral follicles were isolated and encapsulated into alginate hydrogel,then cultured in droplets for 8 days. Experimental groups were treated by different intensity (5 and 450 mT) SMF. Diameter of follicles was measured every other day. The ovulation of antral follicles was induced by HCG hormone,and the matured oocytes (first polar body emission) were identified under the stereomicroscope. RESULTS:Follicles in each group were well developed on morphologically. Follicular diameter of each group gradually increased with culture time. Follicular diameter of 450 mT SMF-treated group was significantly increased at 4 days (P<0.05),and diameter of 5 and 450 mT SMF-treated group was significantly increased at 6 days(P<0.05),compared with that of control group. With the increasing intensity of SMF,the cavity formation rate of the follicles was obviously elevated,but the oocytes maturation rate of 450 mT SMF-treated group was significantly reduced compared with that of the control group. CONCLUSION:The alginate hydrogel could maintains the 3D structure of individual mouse follicle. With increasing strength of SMF,the diameter of secondary follicles was statistically increased,the cavity formation ratio was also markedly increased,but the process of oocyte maturation was inhibited by 450 mT SMF.
