癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2013年
6期
422-425
,共4页
石亚娟%刘辉%刘冉
石亞娟%劉輝%劉冉
석아연%류휘%류염
食管癌细胞%hsa-miR-21-3p%侵袭%迁移
食管癌細胞%hsa-miR-21-3p%侵襲%遷移
식관암세포%hsa-miR-21-3p%침습%천이
esophageal carcinoma cell%hsa-miR-21-3p%invasion%migration
目的：研究hsa-miR-21-3p对人食管癌细胞Eca9706侵袭和迁移能力的影响。方法：采用终浓度为30 nmol/L的寡核苷酸hsa-miR-21-3p mimics和阴性对照转染Eca9706细胞48 h后，用实时荧光定量PCR检测转染后hsa-miR-21-3p的表达水平，通过Transwell体外侵袭试验和划痕愈合试验分别观察hsa-miR-21-3p对Eca9706细胞侵袭和迁移能力的影响。结果：与阴性对照组相比4较，hsa-miR-21-3p mimics转染组细胞中hsa-miR-21-3p表达明显升高(P<0.01)，是阴性对照的1.1×10倍；Transwell体外侵袭试验结果显示镜下每个视野穿膜细胞数试验组(76.67±6.11)较阴性对照组(42.33±2.52)增加了81.1%(P<0.01)，表明hsa-miR-21-3p的过表达显著增强食管癌细胞的侵袭能力；划痕愈合试验结果显示，hsa-miR-21-3p试验组的划痕宽度较对照组窄，在相同的时间点，试验组的划痕愈合能力均大于对照组(P<0.01)，说明转染了hsa-miR-21-3p mimics的试验组细胞的迁移能力大于对照组。结论：Hsa-miR-21-3p的高表达可以使人食管癌细胞Eca9706的侵袭和迁移能力增强，提示其可能参与了食管癌进程中的浸润和转移。
目的：研究hsa-miR-21-3p對人食管癌細胞Eca9706侵襲和遷移能力的影響。方法：採用終濃度為30 nmol/L的寡覈苷痠hsa-miR-21-3p mimics和陰性對照轉染Eca9706細胞48 h後，用實時熒光定量PCR檢測轉染後hsa-miR-21-3p的錶達水平，通過Transwell體外侵襲試驗和劃痕愈閤試驗分彆觀察hsa-miR-21-3p對Eca9706細胞侵襲和遷移能力的影響。結果：與陰性對照組相比4較，hsa-miR-21-3p mimics轉染組細胞中hsa-miR-21-3p錶達明顯升高(P<0.01)，是陰性對照的1.1×10倍；Transwell體外侵襲試驗結果顯示鏡下每箇視野穿膜細胞數試驗組(76.67±6.11)較陰性對照組(42.33±2.52)增加瞭81.1%(P<0.01)，錶明hsa-miR-21-3p的過錶達顯著增彊食管癌細胞的侵襲能力；劃痕愈閤試驗結果顯示，hsa-miR-21-3p試驗組的劃痕寬度較對照組窄，在相同的時間點，試驗組的劃痕愈閤能力均大于對照組(P<0.01)，說明轉染瞭hsa-miR-21-3p mimics的試驗組細胞的遷移能力大于對照組。結論：Hsa-miR-21-3p的高錶達可以使人食管癌細胞Eca9706的侵襲和遷移能力增彊，提示其可能參與瞭食管癌進程中的浸潤和轉移。
목적：연구hsa-miR-21-3p대인식관암세포Eca9706침습화천이능력적영향。방법：채용종농도위30 nmol/L적과핵감산hsa-miR-21-3p mimics화음성대조전염Eca9706세포48 h후，용실시형광정량PCR검측전염후hsa-miR-21-3p적표체수평，통과Transwell체외침습시험화화흔유합시험분별관찰hsa-miR-21-3p대Eca9706세포침습화천이능력적영향。결과：여음성대조조상비4교，hsa-miR-21-3p mimics전염조세포중hsa-miR-21-3p표체명현승고(P<0.01)，시음성대조적1.1×10배；Transwell체외침습시험결과현시경하매개시야천막세포수시험조(76.67±6.11)교음성대조조(42.33±2.52)증가료81.1%(P<0.01)，표명hsa-miR-21-3p적과표체현저증강식관암세포적침습능력；화흔유합시험결과현시，hsa-miR-21-3p시험조적화흔관도교대조조착，재상동적시간점，시험조적화흔유합능력균대우대조조(P<0.01)，설명전염료hsa-miR-21-3p mimics적시험조세포적천이능력대우대조조。결론：Hsa-miR-21-3p적고표체가이사인식관암세포Eca9706적침습화천이능력증강，제시기가능삼여료식관암진정중적침윤화전이。
OBJECTIVE:To explore the potential role of hsa-miR-21-3p in invasion and migration ability of esophageal carcinoma cell line Eca9706. METHODS:Quantitative real-time PCR was used to evaluate the expression level of miR-21-3p in Eca9706 cells and control transfected with 30 nmol/L of hsa-miR-21-3p mimics after 48 h. Transwell and wound healing experiment were carried out to determine cell invasion and migration ability of Eca9706 cells transfected with hsa-miR-21-3p mimics. RESULTS:Hsa-miR-21-3p expression level in hsa-miR-21-3p mimics-4transfected Eca9706 was increased 1.1×10 times compared with negative control. Transwell assay showed the number of transmembrane cells in experimental group was increased 81.1% compared with negative control,suggested that over-expression of hsa-miR-21-3p enhanced the invasion ability of Eca9706 cells. Wound healing experiment demonstrated the capacity of Eca9706 cells migration was strengthened after transfection with hsa-miR-21-3p mimics. CONCLUSION:Up-regulation of hsa-miR-21-3p might serve as an oncogene and participate in the invasion and metastasis of esophageal cancer.
