CAJ | 학술논문

目的：研究不同抗凝管采血对血浆总同型半胱氨酸（tHcy）的影响。方法选取6名健康者，空腹采集静脉全血样本，分别用EDTA-2K管，枸橼酸钠管和肝素锂管采血，不同条件下保存，用HPLC法定量测定tHcy的浓度，用SPSS软件分析处理数据。结果3种抗凝管采血后，即刻分离血浆后4℃放置2 h、4 h、7 h以及-20℃放置1 d、3 d、5 d，不同抗凝管血浆tHcy浓度差异无统计学意义；此外，3种抗凝管采血后，全血4℃放置2 h、4 h、7 h后分离血浆测定血浆tHcy浓度，不同抗凝管差异无统计学意义。结论 EDTA-2K管，枸橼酸钠管和肝素锂管采血对测定的血浆tHcy浓度没有明显差异。
목적：연구불동항응관채혈대혈장총동형반광안산（tHcy）적영향。방법선취6명건강자，공복채집정맥전혈양본，분별용EDTA-2K관，구연산납관화간소리관채혈，불동조건하보존，용HPLC법정량측정tHcy적농도，용SPSS연건분석처리수거。결과3충항응관채혈후，즉각분리혈장후4℃방치2 h、4 h、7 h이급-20℃방치1 d、3 d、5 d，불동항응관혈장tHcy농도차이무통계학의의；차외，3충항응관채혈후，전혈4℃방치2 h、4 h、7 h후분리혈장측정혈장tHcy농도，불동항응관차이무통계학의의。결론 EDTA-2K관，구연산납관화간소리관채혈대측정적혈장tHcy농도몰유명현차이。
Objective To study the influence of different anticoagulant tube of blood for blood plasma total homocysteine (tHcy) . Methods Six healthy subjects were selected, then the fasting venous blood samples were collected with EDTA-2k tube,sodium citrate tube and lithium heparin tube, respectively. The blood samples were kept under different conditions. The concentrations of tHcy were determined by quantitative HPLC method and the data were analyzed using SPSS17.0 software. Results Under the conditions of 4℃placed for 2 h, 4 h, 7 h, and- 20 ℃ placed for 1 d, 3 d, 5 d after separating plasma, the concentrations of plasma tHcy in different anticoagulant tubes were no statistical difference. In addition, after placing the whole blood at 4℃for 2 h, 4 h, 7 h, the concentrations of plasma tHcy of separated plasma in different anticoagulant tubes were no statistical differences. Conclusion There are no significant differences in determination of the concentration of plasma tHcy when using EDTA-2k tube,sodium citrate tube and lithium heparin tube sampling blood.