CAJ | 학술논문

目的：探讨脑干诱发电位对重症急性胰腺炎（severe acute pancreatiti，SAP）大鼠脑损伤的诊断作用．方法60只成年雄性SD大鼠随机分为假手术（sham operation，SO）组和重症急性胰腺炎（SAP）组．两组均于制模后3、6、12 h测量脑干诱发电位变化；透射电镜观察脑组织超微结构的变化；免疫组化和Western Blot检测脑组织Bax、bcl-2和caspase-3蛋白表达．结果与SO组比较，SAP组各时间点脑干诱发电位均呈下降趋势（＜0.05）；透射电镜结果显示SAP组出现明显脑组织损伤变化；SO组Bax、caspase-3蛋白表达显著低于SAP组（＜0.05），bcl-2蛋白表达明显高于SAP组．结论脑干诱发电位对大鼠脑损伤早期诊断灵敏，其结果与大鼠脑损伤程度表现出一致性，可能成为胰性脑病的重要临床诊断指标．
목적：탐토뇌간유발전위대중증급성이선염（severe acute pancreatiti，SAP）대서뇌손상적진단작용．방법60지성년웅성SD대서수궤분위가수술（sham operation，SO）조화중증급성이선염（SAP）조．량조균우제모후3、6、12 h측량뇌간유발전위변화；투사전경관찰뇌조직초미결구적변화；면역조화화Western Blot검측뇌조직Bax、bcl-2화caspase-3단백표체．결과여SO조비교，SAP조각시간점뇌간유발전위균정하강추세（＜0.05）；투사전경결과현시SAP조출현명현뇌조직손상변화；SO조Bax、caspase-3단백표체현저저우SAP조（＜0.05），bcl-2단백표체명현고우SAP조．결론뇌간유발전위대대서뇌손상조기진단령민，기결과여대서뇌손상정도표현출일치성，가능성위이성뇌병적중요림상진단지표．
Objective The aim of this study is to demonstrate the diagnostic effect of brainstem evoked potential for pancreatic encephalopathy in rats with severe acute pancreatitis. Methods Sixty male Sprague-Dawley (SD) rats were randomly divided into two equal groups: a sham-operated (SO) group and a severe acute pancreatitis (SAP) group. Each group was evaluated at 3, 6, and 12 h during the experiment. To detect the brain stem evoked potential change at different time points. The ultrastructure of brain tissue was observed by transmission electron microscope (TEM). The expressions of the apoptosis-related proteins Bcl-2, Bax and caspase-3 were observed using immunohistochemical and Western Blot technique. Results In SAP group, congestion, edema, inflammatory cell infiltration, mitochondrial swelling and cell apoptosis were apparent. Compared with SO group, the brain stem evoked potential in severe acute pancreatitis group was obviously reduced in SAP group. Compared with SAP group, the expressions of Bcl-2 have increased, whereas the expressions of Bax and caspase-3 have decreased in SO group significantly ( <0.05) . Conclusion Brain stem evoked potential is a sensitive method in detection of rat brain damage. The results showed that the consistency and the damage degree of rats may be important clinical diagnostic index of pancreatic encephalopathy.