CAJ | 학술논문

目的：建立小鼠骨髓源树突状细胞（bone marrow dendritic cell， BMDC）的培养方法并对其表型和功能进行鉴定．方法无菌取BALB/c小鼠股骨、胫骨中的骨髓细胞，以粒-巨噬细胞集落刺激因子体外诱导分化为BMDC，倒置显微镜动态观察BMDC增殖和形态变化情况，流式细胞术分析细胞表型，并检测其抗原吞噬功能．结果小鼠骨髓细胞体外诱导可获得大量未成熟和成熟BMDC，呈现典型的树突状形态．未成熟BMDC的细胞表型为CD11chighCD40lowCD86low MHC-IIlow，具有较强的抗原吞噬能力．未成熟BMDC经细菌脂多糖刺激后可分化为高表达CD11c、CD40、CD86及MHC-II类分子的成熟BMDC．结论体外诱导培养可获得小鼠骨髓来源的未成熟和成熟DC．
목적：건립소서골수원수돌상세포（bone marrow dendritic cell， BMDC）적배양방법병대기표형화공능진행감정．방법무균취BALB/c소서고골、경골중적골수세포，이립-거서세포집락자격인자체외유도분화위BMDC，도치현미경동태관찰BMDC증식화형태변화정황，류식세포술분석세포표형，병검측기항원탄서공능．결과소서골수세포체외유도가획득대량미성숙화성숙BMDC，정현전형적수돌상형태．미성숙BMDC적세포표형위CD11chighCD40lowCD86low MHC-IIlow，구유교강적항원탄서능력．미성숙BMDC경세균지다당자격후가분화위고표체CD11c、CD40、CD86급MHC-II류분자적성숙BMDC．결론체외유도배양가획득소서골수래원적미성숙화성숙DC．
Objective To establish a method of cultivation of dendritic cells (DC) from mouse bone marrow in vitro and identify their phenotype and function. Methods Under aseptic condition, bone marrow cells were extracted from the tibia and femur bones of BALB/c mice. Bone marrow cells were cultured with recombinant mouse granulocyte-macrophage colony-stimulating factor ( rmGM-CSF) in vitro. The expansion and morphological changes of DC were observed with light inverted microscope. Phenotype was identified with flow cytometry and biological function was studied with antigen phagocytosis test. Results A large number of immature and mature DC with typical dendritic morphological characteristics could be generated from murine bone marrow. Immature DC, which had high expression in CD11c and low expression in CD40, MHC-II and CD86, could phagocytize antigen. Mature DC, which could be induced from immature DC by lipopolysaccharides, had high expression in CD11c, CD40, CD86 and MHC-II molecules. Conclusion Immature and mature DC can be generated from mouse bone marrow cells through cytokine induction in vitro and be used for further study associated with DC.