CAJ | 학술논문

目的：通过硝酸镓、顺铂单药及二者联合用药对胃癌BGC823细胞作用，观察其对胃癌BGC823细胞诱导凋亡、抑制增殖的影响。方法通过对胃腺癌BGC823细胞培养及干预，采用流式细胞仪技术对凋亡细胞进行测定；MTT技术对肿瘤细胞增殖进行测定。结果 MTT及流式细胞仪检测结果显示：硝酸镓对BGC823细胞作用呈剂量-时间依赖效应；小剂量硝酸镓与顺铂配伍应用能大大提高顺铂作用效应，配伍高浓度组在24 h、48 h、72 h的抑制率为70.0%、71.4%、72.3%。结论硝酸镓对胃癌细胞的作用与顺铂无明显差异，为肿瘤治疗及制备抗肿瘤药物提供新的途径。
목적：통과초산가、순박단약급이자연합용약대위암BGC823세포작용，관찰기대위암BGC823세포유도조망、억제증식적영향。방법통과대위선암BGC823세포배양급간예，채용류식세포의기술대조망세포진행측정；MTT기술대종류세포증식진행측정。결과 MTT급류식세포의검측결과현시：초산가대BGC823세포작용정제량-시간의뢰효응；소제량초산가여순박배오응용능대대제고순박작용효응，배오고농도조재24 h、48 h、72 h적억제솔위70.0%、71.4%、72.3%。결론초산가대위암세포적작용여순박무명현차이，위종류치료급제비항종류약물제공신적도경。
Objective To assess the inhibitory effect of gallium nitrate, cisplatin and their combination on proliferation and apoptosis of gastric cancer (BGC823) cells.Methods The BGC823 cells were cultured and treated with gallium nitrate, cisplatin or their combination. Apoptosis and proliferation of BGC823 cells were detected byflow cytometry and MTT assay, respectively. Results MTT assay andflow cytmetry showed that gallium nitrate inhibited the proliferation of BGC823 cells in a time- and dose-dependent manner. Small dose of combined gallium nitrate and cisplatin could greatly increase the inhibitory effect of cisplatin on the proliferation and apoptosis of BGC823 cells. The inhibition rate of combined gallium nitrate and cisplatin was 70.0%, 71.4% and 72.3% at 24 h, 48 hand 72 h, respectively.Conclusion The effect of gallium nitrate and cisplatin on proliferation and apoptosis of BGC823 cells is not significantly different and can thus provide a new approach to the drug treatment of gastric cancer and to the preparation of anti-tumor drugs.