解放军医学院学报
解放軍醫學院學報
해방군의학원학보
Academic Journal of Chinese Pla Medical School
2013年
11期
1174-1177
,共4页
锌%神经病理性疼痛%脊髓%磷酸化%cAMP反应元件结合蛋白
鋅%神經病理性疼痛%脊髓%燐痠化%cAMP反應元件結閤蛋白
자%신경병이성동통%척수%린산화%cAMP반응원건결합단백
zinc%neuropathic pain%spinal cord%phosphorylative%camp response-element binding protein
目的：研究锌对神经病理性疼痛(neuropathic pain，NPP)模型小鼠脊髓磷酸化(phosphoryltion，p)的cAMP反应元件结合蛋白(camp response-element binding protein，CREB)表达的影响。方法72只C57/BL6小鼠随机分为4组，正常喂养的NPP组锌饲料30 mg/(kg·d)喂养2周，低锌喂养的NPP组锌饲料0.85 mg/(kg·d)喂养2周，高锌喂养的NPP组用硫酸锌水溶液227 mg/(L·d)喂养2周。采用足底注射辣椒素(0.5%5μl)制备NPP模型：对照组锌饲料30 mg/(kg·d)喂养2周后足底注射溶剂。应用原子吸收光谱、免疫组织化学、免疫印迹和图像分析技术检测术后7 d锌对脊髓pCREB表达的影响。结果高锌喂养能显著增加血清和脊髓中锌的含量，脊髓pCREB表达下调(P＜0.01)；而低锌喂养加重血清和脊髓的缺锌状况，脊髓pCREB表达上调(P＜0.01)。结论锌能抑制NPP模型小鼠脊髓pCREB的表达。
目的：研究鋅對神經病理性疼痛(neuropathic pain，NPP)模型小鼠脊髓燐痠化(phosphoryltion，p)的cAMP反應元件結閤蛋白(camp response-element binding protein，CREB)錶達的影響。方法72隻C57/BL6小鼠隨機分為4組，正常餵養的NPP組鋅飼料30 mg/(kg·d)餵養2週，低鋅餵養的NPP組鋅飼料0.85 mg/(kg·d)餵養2週，高鋅餵養的NPP組用硫痠鋅水溶液227 mg/(L·d)餵養2週。採用足底註射辣椒素(0.5%5μl)製備NPP模型：對照組鋅飼料30 mg/(kg·d)餵養2週後足底註射溶劑。應用原子吸收光譜、免疫組織化學、免疫印跡和圖像分析技術檢測術後7 d鋅對脊髓pCREB錶達的影響。結果高鋅餵養能顯著增加血清和脊髓中鋅的含量，脊髓pCREB錶達下調(P＜0.01)；而低鋅餵養加重血清和脊髓的缺鋅狀況，脊髓pCREB錶達上調(P＜0.01)。結論鋅能抑製NPP模型小鼠脊髓pCREB的錶達。
목적：연구자대신경병이성동통(neuropathic pain，NPP)모형소서척수린산화(phosphoryltion，p)적cAMP반응원건결합단백(camp response-element binding protein，CREB)표체적영향。방법72지C57/BL6소서수궤분위4조，정상위양적NPP조자사료30 mg/(kg·d)위양2주，저자위양적NPP조자사료0.85 mg/(kg·d)위양2주，고자위양적NPP조용류산자수용액227 mg/(L·d)위양2주。채용족저주사랄초소(0.5%5μl)제비NPP모형：대조조자사료30 mg/(kg·d)위양2주후족저주사용제。응용원자흡수광보、면역조직화학、면역인적화도상분석기술검측술후7 d자대척수pCREB표체적영향。결과고자위양능현저증가혈청화척수중자적함량，척수pCREB표체하조(P＜0.01)；이저자위양가중혈청화척수적결자상황，척수pCREB표체상조(P＜0.01)。결론자능억제NPP모형소서척수pCREB적표체。
Objective To study the effect of zinc on expression of phosphorylated camp response-element binding protein (pCREB) in spinal cord of a mouse neuropathic pain (NPP) model.Methods Seventy-two C57/BL6 mice were divided into normal NPP group, low zinc NPP group, high zinc NPP group, and control group (18 in each group) and fed with 30 mg zinc (kg·d), 0.85 mg zinc (kg·d), 227 mg zinc sulfate water solution (L·d), and 30 mg forage (kg·d) for 2 weeks, respectively. Seven days after a mouse NPP model was established by injecting 0.5% 5 μl capsaicin into the planta (control group: injected solvent), effect of zinc on expression of pCREB in spinal cord of mice was detected by atomic absorption spectrum, immunohistochemistry, immunoblotting, and image analysis, respectively.Results The zinc level in spinal cord and serum were all significantly higher while the expression level of pCREB in spinal cord was significantly lower in high zinc NPP group than in other groups (P<0.01). The zinc level in spinal cord and serum were all significantly lower while the expression level of pCREB in spinal cord was significantly higher in low zinc NPP group than in other groups (P<0.01).Conclusion Zinc can inhibit the expression of pCREB in spinal cord of mouse NPP model.
