CAJ | 학술논문

探讨TCTE3 mRNA在人肝癌细胞株中的表达水平及意义。采用实时荧光定量PCR技术分别检测TCTE3基因在常规培养或缺氧诱导（CoCl2为诱导剂）的人肝癌细胞株中的表达情况。TCTE3 mRNA在常规培养的人正常肝细胞株L02，肝癌细胞株Bel-7402、SMMC-7721、HepG2和QGY-7701中不表达或极低表达；但经缺氧诱导后，SMMC-7721细胞中的TCTE3 mRNA表达量逐渐升高，4h达到最大，随后逐渐降低，与HIF1a mRNA表达的曲线变化有一定相似之处，只是这种变化的时间要早于HIF1a基因。在缺氧状态下肝癌细胞TCTE3 mRNA表达增加，且这种上调表达可能对HIF1a mRNA的表达有一定的促进作用。
탐토TCTE3 mRNA재인간암세포주중적표체수평급의의。채용실시형광정량PCR기술분별검측TCTE3기인재상규배양혹결양유도（CoCl2위유도제）적인간암세포주중적표체정황。TCTE3 mRNA재상규배양적인정상간세포주L02，간암세포주Bel-7402、SMMC-7721、HepG2화QGY-7701중불표체혹겁저표체；단경결양유도후，SMMC-7721세포중적TCTE3 mRNA표체량축점승고，4h체도최대，수후축점강저，여HIF1a mRNA표체적곡선변화유일정상사지처，지시저충변화적시간요조우HIF1a기인。재결양상태하간암세포TCTE3 mRNA표체증가，차저충상조표체가능대HIF1a mRNA적표체유일정적촉진작용。
To investigate genes expression and significance of TCTE3 mRNA in liver cancer cells cultured. TCTE3 mRNA were examined in liver cancer cells under conventional culture or hypoxia-induced culture by CoCl2 by real-time quantitative Polymerase chain reaction (PCR). No expression or low expression of TCTE3 mRNA levels in normal human liver L02 cells, and liver cancer Bel-7402, SMMC-7721, HepG2 or QGY-7701 cells. However, After SMMC-7721 cells were incubated with hypoxia-inducible CoCl2, the expression of TCTE3 mRNA began to increase gradually, peaked at 4h, and then decreased, which has some similarities with HIF1a mRNA expression curve, but such changes earlier than HIF1a mRNA. The expression of TCTE3 mRNA levels increased, which could promote HIF1a mRNA expression.