中国骨与关节杂志
中國骨與關節雜誌
중국골여관절잡지
Chinese Journal of Bone and Joint
2013年
12期
712-715
,共4页
丁舒晨%马超然%王哲洋%江友泉%李政%田臻%邱裕生%尹战海
丁舒晨%馬超然%王哲洋%江友泉%李政%田臻%邱裕生%尹戰海
정서신%마초연%왕철양%강우천%리정%전진%구유생%윤전해
肩关节%肌腱%创伤和损伤%前列腺素
肩關節%肌腱%創傷和損傷%前列腺素
견관절%기건%창상화손상%전렬선소
Shoulder joint%Tendinopathy%Wounds and injuries%Prostaglandins
目的探讨前列腺素 E2( prostaglandin E2，PGE2)在肩袖损伤中的作用。方法标本分为5组：撕裂组8例，钙化性冈上肌腱炎组11例，新鲜尸体组6例，阳性对照组和阴性对照组各10例。其中前3组为冈上肌腱标本，阳性对照组和阴性对照组为重度骨性关节炎滑膜标本。同时在阴性对照组中将一抗替换为PBS缓冲液。所有标本均采用微波热修复抗原，一抗使用ABCAM公司兔抗人PGE2单克隆抗体，1∶100稀释后使用于免疫组化法染色。人工定位所有细胞集中区域，以400倍照相显微镜采集图像，并在图像编号后采用计算机随机数生成软件随机抽取8个编号所对应的高倍视野(×400)图像，用IPP6.0图像处理软件，以除去细胞核外的所有区域为感兴趣区，测量面积和光密度，并计算平均光密度( mean optical density，MOD )以评价PGE2表达量，并对结果进行t检验，其中α＝0.05。结果阴性对照组组织细胞质和细胞外基质均呈阴性染色；阳性对照组组织细胞质呈强阳性染色，基质呈弱阳性染色；新鲜尸体组细胞质和基质均呈阴性染色；钙化性冈上肌腱炎组细胞质及基质均呈弱阳性染色；撕裂组细胞质呈强阳性染色，基质呈弱阳性染色。肩袖撕裂组平均光密度为0.3158±0.0204，钙化性冈上肌腱炎组平均光密度为0.0907±0.0160，新鲜尸体组平均光密度为0.0404±0.0096。撕裂组与新鲜尸体组比较，PGE2表达明显增高，差异有统计学意义( P＜0.001)。钙化性冈上肌腱炎组与新鲜尸体组比较，PGE2表达轻度增高，差异有统计学意义( P＜0.001)。撕裂组较钙化性冈上肌腱炎组，PGE2显著增高，差异有统计学意义( P＜0.001)。结论肩袖损伤患者冈上肌腱中PGE2呈高表达，提示肩袖损伤与PGE2高表达相关。
目的探討前列腺素 E2( prostaglandin E2，PGE2)在肩袖損傷中的作用。方法標本分為5組：撕裂組8例，鈣化性岡上肌腱炎組11例，新鮮尸體組6例，暘性對照組和陰性對照組各10例。其中前3組為岡上肌腱標本，暘性對照組和陰性對照組為重度骨性關節炎滑膜標本。同時在陰性對照組中將一抗替換為PBS緩遲液。所有標本均採用微波熱脩複抗原，一抗使用ABCAM公司兔抗人PGE2單剋隆抗體，1∶100稀釋後使用于免疫組化法染色。人工定位所有細胞集中區域，以400倍照相顯微鏡採集圖像，併在圖像編號後採用計算機隨機數生成軟件隨機抽取8箇編號所對應的高倍視野(×400)圖像，用IPP6.0圖像處理軟件，以除去細胞覈外的所有區域為感興趣區，測量麵積和光密度，併計算平均光密度( mean optical density，MOD )以評價PGE2錶達量，併對結果進行t檢驗，其中α＝0.05。結果陰性對照組組織細胞質和細胞外基質均呈陰性染色；暘性對照組組織細胞質呈彊暘性染色，基質呈弱暘性染色；新鮮尸體組細胞質和基質均呈陰性染色；鈣化性岡上肌腱炎組細胞質及基質均呈弱暘性染色；撕裂組細胞質呈彊暘性染色，基質呈弱暘性染色。肩袖撕裂組平均光密度為0.3158±0.0204，鈣化性岡上肌腱炎組平均光密度為0.0907±0.0160，新鮮尸體組平均光密度為0.0404±0.0096。撕裂組與新鮮尸體組比較，PGE2錶達明顯增高，差異有統計學意義( P＜0.001)。鈣化性岡上肌腱炎組與新鮮尸體組比較，PGE2錶達輕度增高，差異有統計學意義( P＜0.001)。撕裂組較鈣化性岡上肌腱炎組，PGE2顯著增高，差異有統計學意義( P＜0.001)。結論肩袖損傷患者岡上肌腱中PGE2呈高錶達，提示肩袖損傷與PGE2高錶達相關。
목적탐토전렬선소 E2( prostaglandin E2，PGE2)재견수손상중적작용。방법표본분위5조：시렬조8례，개화성강상기건염조11례，신선시체조6례，양성대조조화음성대조조각10례。기중전3조위강상기건표본，양성대조조화음성대조조위중도골성관절염활막표본。동시재음성대조조중장일항체환위PBS완충액。소유표본균채용미파열수복항원，일항사용ABCAM공사토항인PGE2단극륭항체，1∶100희석후사용우면역조화법염색。인공정위소유세포집중구역，이400배조상현미경채집도상，병재도상편호후채용계산궤수궤수생성연건수궤추취8개편호소대응적고배시야(×400)도상，용IPP6.0도상처리연건，이제거세포핵외적소유구역위감흥취구，측량면적화광밀도，병계산평균광밀도( mean optical density，MOD )이평개PGE2표체량，병대결과진행t검험，기중α＝0.05。결과음성대조조조직세포질화세포외기질균정음성염색；양성대조조조직세포질정강양성염색，기질정약양성염색；신선시체조세포질화기질균정음성염색；개화성강상기건염조세포질급기질균정약양성염색；시렬조세포질정강양성염색，기질정약양성염색。견수시렬조평균광밀도위0.3158±0.0204，개화성강상기건염조평균광밀도위0.0907±0.0160，신선시체조평균광밀도위0.0404±0.0096。시렬조여신선시체조비교，PGE2표체명현증고，차이유통계학의의( P＜0.001)。개화성강상기건염조여신선시체조비교，PGE2표체경도증고，차이유통계학의의( P＜0.001)。시렬조교개화성강상기건염조，PGE2현저증고，차이유통계학의의( P＜0.001)。결론견수손상환자강상기건중PGE2정고표체，제시견수손상여PGE2고표체상관。
Objective To investigate the effects of prostaglandin E2 ( PGE2 ) on rotator cuff injuries. Methods The samples were divided into 5 groups, including tear group ( n=8 ), calciifed supraspinatus tendonitis group ( n=11 ), fresh cadaver group ( n=6 ), positive control group ( n=10 ) and negative control group ( n=10 ). The samples of the former 3 groups were tissues of supraspinatus tendons. The samples of the positive control group and negative control group were synoviums got from patients with serious osteoarthritis, and in the negative control group phosphate buffered saline ( PBS ) solution was used instead of primary antibodies. Before being stained with immunohistochemistry, all samples were detected by using microwave antigen retrieval. The primary antibodies were rabbit anti-human PGE2 monoclonal antibodies from ABCAM Company, which were diluted for 100 times. All the areas of cell aggregation were manually located, and the images were collected with a photomicroscope ( ×400 ). After the images were numbered, the computer random number generation software was utilized to select 8 numbered high power ifelds ( ×400 ) randomly. When all the areas except for the cell nucleus were taken as the regions of interest, the area and absorbance were measured by the Image-Pro Plus 6.0 ( IPP6.0 ) image processing software, and the mean optical density ( MOD ) was calculated. After that the expressions of PGE2 were evaluated, and the results were studied by t-test (α=0.05 ). Results The cytoplasm and extracellular matrix in the negative control group were both stained negatively, which were the same in the fresh cadaver group. In the positive control group, the cytoplasm and matrix showed strongly and weakly positive staining respectively, which were the same in the tear group. The cytoplasm and matrix in the calciifed supraspinatus tendonitis group both showed weakly positive staining. The MOD in the tear group was 0.3158±0.0204, which was 0.0907±0.0160 in the calcified supraspinatus tendonitis group and 0.0404±0.0096 in the fresh cadaver group. The expressions of PGE2 in the tear group were signiifcantly higher than that in the fresh cadaver group, and the differences were statistically signiifcant ( P<0.001 ). The expressions of PGE2 in the calciifed supraspinatus tendonitis group were slightly higher than that in the fresh cadaver group, and the differences were statistically signiifcant ( P<0.001 ). The expressions of PGE2 in the tear group were signiifcantly higher than that in the calciifed supraspinatus tendonitis group, and the differences were statistically signiifcant ( P<0.001 ). Conclusions PGE2 is highly expressed in the supraspinatus tendons of patients with rotator cuff injuries, which means the rotator cuff injuries are connected with the high expressions of PGE2 to some extent.
