CAJ | 학술논문

目的：对H7 N9型禽流感病毒（简称H7 N9病毒）监测病例进行病原学检测，以明确呼吸道感染的诊断与鉴别诊断。方法采用多重逆转录聚合酶链反应（RT-PCR）对H7 N9病毒进行监测的同时对22种呼吸道常见病原体核酸进行检测，并对检测到的病原体进行分离鉴定。结果在一例重症肺炎患者的H7 N9病毒监测标本中检出A型呼吸道合胞病毒（RSV-A），并利用人胚肺成纤维细胞（MRC-5）对该标本进行了病毒分离，成功分离到了一株RSV-A。结论多重RT-PCR能同时对22种呼吸道病原核酸进行检测，有助于疫情的及时诊断和控制。RSV-A的成功分离为呼吸道合胞病毒的进一步研究打下基础。
목적：대H7 N9형금류감병독（간칭H7 N9병독）감측병례진행병원학검측，이명학호흡도감염적진단여감별진단。방법채용다중역전록취합매련반응（RT-PCR）대H7 N9병독진행감측적동시대22충호흡도상견병원체핵산진행검측，병대검측도적병원체진행분리감정。결과재일례중증폐염환자적H7 N9병독감측표본중검출A형호흡도합포병독（RSV-A），병이용인배폐성섬유세포（MRC-5）대해표본진행료병독분리，성공분리도료일주RSV-A。결론다중RT-PCR능동시대22충호흡도병원핵산진행검측，유조우역정적급시진단화공제。RSV-A적성공분리위호흡도합포병독적진일보연구타하기출。
Objective To detect and diagnose respiratory pathogens in the monitoring specimens of avian influenza A (H7N9)virus.Methods Nucleic acids of 22 kinds of respiratory pathogens were determined by multiple reverse transcription polymerase chain reaction (RT-PCR).Relative respiratory pathogens were isolated and indentified. Results Among the monitoring specimens of avian influenza A (H7N9)virus,nucleic acid of respiratory syncytial virus A (RSV-A)was determined from a severe pneumonia illness patient.The specimen was isolated by MRC-5 ,and 1 strain of RSV-A was isolated successfully.Conclusions Multiple RT-PCR could test 22 kinds of respiratory pathogens simultaneously and contributes to diagnose and control the epidemic situation.The isolation of RSV-A would provide a foothold for the further research of respiratory syncytial virus.