CAJ | 학술논문

目的：了解肝细胞癌（HCC）患者外周血单个核细胞（PBMC）人类白细胞抗原G（HLA-G）的表达水平。方法收集38例HCC患者、25例肝硬化患者和40名健康查体者的PBMC，分别用逆转录-聚合酶链反应（PCR）和流式细胞术（FCM）检测其HLA-G mRNA和HLA-G蛋白表达水平。结果 HCC组、肝硬化组和健康对照组PBMC HLA-G mRNA表达率分别为94．7％、84．0％和87．5％，差异无统计学意义（P＝0．359）；HCC组HLA-G蛋白表达率高于肝硬化组和健康对照组，差异有统计学意义（P＝0．002）。HCC组PBMC HLA-G蛋白主要表达于CD4＋细胞上。结论 HCC患者PBMC HLA-G蛋白表达率升高，其机制与临床意义尚待进一步研究。
목적：료해간세포암（HCC）환자외주혈단개핵세포（PBMC）인류백세포항원G（HLA-G）적표체수평。방법수집38례HCC환자、25례간경화환자화40명건강사체자적PBMC，분별용역전록-취합매련반응（PCR）화류식세포술（FCM）검측기HLA-G mRNA화HLA-G단백표체수평。결과 HCC조、간경화조화건강대조조PBMC HLA-G mRNA표체솔분별위94．7％、84．0％화87．5％，차이무통계학의의（P＝0．359）；HCC조HLA-G단백표체솔고우간경화조화건강대조조，차이유통계학의의（P＝0．002）。HCC조PBMC HLA-G단백주요표체우CD4＋세포상。결론 HCC환자PBMC HLA-G단백표체솔승고，기궤제여림상의의상대진일보연구。
Objective To investigate the expression level of peripheral blood mononuclear cell (PBMC)human leucocyte antigen G (HLA-G)in patients with hepatocellular carcinoma (HCC).Methods The HLA-G mRNA and HLA-G protein expression levels on PBMC from 38 patients with HCC,25 patients with liver cirrhosis and 40 healthy subjects were determined by reverse transcription-polymerase chain reaction (PCR)and flow cytometry (FCM).Results The HLA-G mRNA expression rates in HCC group,cirrhosis group and healthy control group were 94.7%,84.0% and 87.5%,respectively,and there was no statistical significance (P=0.359).The HLA-G protein expression rate in HCC group was significantly higher than those in cirrhosis and healthy control groups with statistical significance (P=0.002). In HCC group,the HLA-G protein was mainly expressed on CD4+ cells.Conclusions The PBMC HLA-G protein expression rate in HCC patients increases,and its mechanism and clinical significance need to be further researched.