中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
18期
8335-8338
,共4页
李峻岭%姜盛花%段颖欣%韩英
李峻嶺%薑盛花%段穎訢%韓英
리준령%강성화%단영흔%한영
肌细胞,平滑肌%硫酸吲哚酚%辛伐他汀%氧化应激%钙化
肌細胞,平滑肌%硫痠吲哚酚%辛伐他汀%氧化應激%鈣化
기세포,평활기%류산신타분%신벌타정%양화응격%개화
Myocytes,smooth muscle%Indoxyl sulfate%Simvastatin%Oxidative stress%Calcification
目的：观察硫酸吲哚酚（indoxyl sulfate，IS）对大鼠血管平滑肌细胞（vascular smooth muscle cell，VSMC）钙化的影响以及这种变化和氧化应激的关系。方法培养的 VSMC 分别加入100μmol/L、300μmol/L和500μmol/L IS，每组再分别加入10μmol/L辛伐他汀。应用甲σ-酚肽络合酮法检测细胞内的钙含量，硫代巴比妥酸（TBA）法检测上清液中丙二醛（MDA）含量。结果与正常组相比，IS 300μmol/L组含钙量[（10.96±3.05）mg/g 蛋白 vs.（7.52±1.51）mg/g 蛋白，P＜0.05]和 IS 500μmol/L 组含钙量[（14.34±4.31）mg/g蛋白vs.（7.52±1.51）mg/g蛋白，P＜0.01]均明显增高。与同浓度IS组相比，辛伐他汀10μmol/L＋IS 300μmol/L组和辛伐他汀10μmol/L＋IS 500μmol/L组含钙量均明显降低[（7.46±1.86） mg/g蛋白vs.（10.96±3.05）mg/g蛋白；（8.83±2.76）mg/g蛋白vs.（14.34±4.31）mg/g蛋白，均P＜0.05]。与正常组相比，IS 300μmol/L组（2.52±0.47 vs.1.54±0.23，P＜0.01）和IS 500μmol/L组上清液MDA含量（2.72±0.53 vs.1.54±0.23，P＜0.01）均明显增高。与同浓度IS组相比，辛伐他汀10μmol/L＋IS 300μmol/L组和辛伐他汀10μmol/L＋IS 500μmol/L 组上清液 MDA 含量均明显降低（1.54±0.39 vs.2.52±0.47，1.60±0.39 vs.2.72±0.53，均P＜0.01）。大鼠VSMC的含钙量与MDA呈正相关关系（r＝0.59，P＜0.01）。结论 IS可浓度依赖性地促进大鼠VSMC钙化，其作用可能与IS增加VSMC的氧化应激有关，辛伐他汀可抑制此作用。
目的：觀察硫痠吲哚酚（indoxyl sulfate，IS）對大鼠血管平滑肌細胞（vascular smooth muscle cell，VSMC）鈣化的影響以及這種變化和氧化應激的關繫。方法培養的 VSMC 分彆加入100μmol/L、300μmol/L和500μmol/L IS，每組再分彆加入10μmol/L辛伐他汀。應用甲σ-酚肽絡閤酮法檢測細胞內的鈣含量，硫代巴比妥痠（TBA）法檢測上清液中丙二醛（MDA）含量。結果與正常組相比，IS 300μmol/L組含鈣量[（10.96±3.05）mg/g 蛋白 vs.（7.52±1.51）mg/g 蛋白，P＜0.05]和 IS 500μmol/L 組含鈣量[（14.34±4.31）mg/g蛋白vs.（7.52±1.51）mg/g蛋白，P＜0.01]均明顯增高。與同濃度IS組相比，辛伐他汀10μmol/L＋IS 300μmol/L組和辛伐他汀10μmol/L＋IS 500μmol/L組含鈣量均明顯降低[（7.46±1.86） mg/g蛋白vs.（10.96±3.05）mg/g蛋白；（8.83±2.76）mg/g蛋白vs.（14.34±4.31）mg/g蛋白，均P＜0.05]。與正常組相比，IS 300μmol/L組（2.52±0.47 vs.1.54±0.23，P＜0.01）和IS 500μmol/L組上清液MDA含量（2.72±0.53 vs.1.54±0.23，P＜0.01）均明顯增高。與同濃度IS組相比，辛伐他汀10μmol/L＋IS 300μmol/L組和辛伐他汀10μmol/L＋IS 500μmol/L 組上清液 MDA 含量均明顯降低（1.54±0.39 vs.2.52±0.47，1.60±0.39 vs.2.72±0.53，均P＜0.01）。大鼠VSMC的含鈣量與MDA呈正相關關繫（r＝0.59，P＜0.01）。結論 IS可濃度依賴性地促進大鼠VSMC鈣化，其作用可能與IS增加VSMC的氧化應激有關，辛伐他汀可抑製此作用。
목적：관찰류산신타분（indoxyl sulfate，IS）대대서혈관평활기세포（vascular smooth muscle cell，VSMC）개화적영향이급저충변화화양화응격적관계。방법배양적 VSMC 분별가입100μmol/L、300μmol/L화500μmol/L IS，매조재분별가입10μmol/L신벌타정。응용갑σ-분태락합동법검측세포내적개함량，류대파비타산（TBA）법검측상청액중병이철（MDA）함량。결과여정상조상비，IS 300μmol/L조함개량[（10.96±3.05）mg/g 단백 vs.（7.52±1.51）mg/g 단백，P＜0.05]화 IS 500μmol/L 조함개량[（14.34±4.31）mg/g단백vs.（7.52±1.51）mg/g단백，P＜0.01]균명현증고。여동농도IS조상비，신벌타정10μmol/L＋IS 300μmol/L조화신벌타정10μmol/L＋IS 500μmol/L조함개량균명현강저[（7.46±1.86） mg/g단백vs.（10.96±3.05）mg/g단백；（8.83±2.76）mg/g단백vs.（14.34±4.31）mg/g단백，균P＜0.05]。여정상조상비，IS 300μmol/L조（2.52±0.47 vs.1.54±0.23，P＜0.01）화IS 500μmol/L조상청액MDA함량（2.72±0.53 vs.1.54±0.23，P＜0.01）균명현증고。여동농도IS조상비，신벌타정10μmol/L＋IS 300μmol/L조화신벌타정10μmol/L＋IS 500μmol/L 조상청액 MDA 함량균명현강저（1.54±0.39 vs.2.52±0.47，1.60±0.39 vs.2.72±0.53，균P＜0.01）。대서VSMC적함개량여MDA정정상관관계（r＝0.59，P＜0.01）。결론 IS가농도의뢰성지촉진대서VSMC개화，기작용가능여IS증가VSMC적양화응격유관，신벌타정가억제차작용。
Objective To investigate the effect of indoxyl sulfate (IS) on calcification in rat vascular smooth muscle cell (VSMC) and the relationship between oxidative stress and this effect. Methods Cultured rat VSMCs were incubated with 100 μmol/L, 300 μmol/L and 500 μmol/L IS, respectively. In some experiments, VSMC cells were incubated with 10 μmol/L simvastatin. Calcium deposition in cells was measured byσ-cresolphthalein complexone method.The malondialdehyde (MDA) content was detected by thiobarbituric acid method. Results Compared with calcium deposition and MDA levels of supernatant in normal cultured VSMCs, those in VSMCs incubated with 300μmol/L IS[(10.96±3.05) mg/g protein vs. (7.52±1.51) mg/g protein, P<0.05 and 2.52±0.47 vs. 1.54±0.23, P<0.01]and 500 μmol/L IS[(14.34±4.31) mg/g protein vs. (7.52±1.51) mg/g protein, P<0.01 and 2.72±0.53 vs. 1.54±0.23, P<0.01]were significantly increased. Compared with calcium deposition and MDA levels of supernatant in VSMCs incubated with 300μmol/L IS and 500μmol/L IS, those in VSMCs in 300μmol/L IS[(7.46±1.86) mg/g protein vs. (10.96±3.05) mg/g protein, P<0.05 and 1.54±0.39 vs. 2.52±0.47, P<0.01]and 500μmol/L IS[(8.83±2.76) mg/g protein vs. (14.34±4.31) mg/g protein, P<0.05 and 1.60±0.39 vs. 2.72±0.53, P<0.01] incubated with 10 μmol/L simvastatin were significantly decreased. There was significantly positive correlation between calcium deposition and MDA content(r=0.59, P<0.001). Conclusion IS may promote calcification in rat VSMC with concentration-dependent manner, which may be possibly related with the increased oxidative stress induced by IS. Simvastatin may inhibit this effect.
