中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
18期
8312-8316
,共5页
大鼠,Wistar%血管内皮生长因子类%肝细胞生长因子%酶联免疫吸附测定%虹膜新生血管
大鼠,Wistar%血管內皮生長因子類%肝細胞生長因子%酶聯免疫吸附測定%虹膜新生血管
대서,Wistar%혈관내피생장인자류%간세포생장인자%매련면역흡부측정%홍막신생혈관
Rats,Wistar%Vascular endothelial growth factor%Hepatocyte growth factor%Enzyme-linked immunosorbent assay%Neovascularization of the iris
目的:探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)、肝细胞生长因子(hepatocyte growth factor,HGF)在虹膜新生血管(neovascularization of the iris,NVI)大鼠模型房水中的含量变化。方法将清洁级健康10周龄Wistar大鼠随机分为4组,每组20只(40眼),乙醚吸入法全身麻醉大鼠,实验1组:经尾部静脉注射孟加拉玫瑰红光敏剂;实验2组:尾部静脉注射孟加拉玫瑰红光敏剂+氪激光光凝Wistar大鼠全部视网膜分支静脉;实验3组:氪激光光凝Wistar大鼠全部视网膜分支静脉;实验4组:空白对照组(未经任何处理)。利用虹膜荧光造影(iris fluorescence angiography,IFA)对各实验组大鼠进行鉴定,对NVI动物模型复制成功的大鼠采用Miller方法进行分级,对大鼠NVI形成前1天及模型复制后3、7、10、14、21、30 d分别抽取房水,采用酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)检测房水中VEGF、HGF的含量。采用SPSS 13.0统计软件分析实验组及对照组之间不同时间组房水中VEGF、HGF 含量变化,并分析两种因子的相关性。结果单纯应用孟加拉玫瑰红尾部静脉注射和单纯激光的方法不能使大鼠产生视网膜缺血,用尾部静脉注射孟加拉玫瑰红光敏剂+氪激光光凝法可诱导大鼠视网膜静脉阻塞,成功制作NVI动物模型。 NVI模型复制前1 d房水中VEGF和HGF的浓度分别为(15.58±5.85)pg/ml和(22.84±6.75)pg/ml,复制后3、7、10、14、21、30 d二者浓度分别为(17.30±4.80)、(31.83±9.90)、(36.89±11.73)、(57.34±18.55)、(112.03±46.41)、(106.93±52.89)pg/ml和(24.58±7.18)、(36.47±11.28)、(46.27±13.11)、(59.25±15.47)、(90.71±29.91)、(102.08±34.82)pg/ml;VEGF和HGF与NVI形成呈正相关,但二者浓度无相关性。结论大鼠虹膜新生血管的形成与其房水中VEGF、HGF含量成正相关,二者在虹膜新生血管形成中可能扮演重要角色。
目的:探討血管內皮生長因子(vascular endothelial growth factor,VEGF)、肝細胞生長因子(hepatocyte growth factor,HGF)在虹膜新生血管(neovascularization of the iris,NVI)大鼠模型房水中的含量變化。方法將清潔級健康10週齡Wistar大鼠隨機分為4組,每組20隻(40眼),乙醚吸入法全身痳醉大鼠,實驗1組:經尾部靜脈註射孟加拉玫瑰紅光敏劑;實驗2組:尾部靜脈註射孟加拉玫瑰紅光敏劑+氪激光光凝Wistar大鼠全部視網膜分支靜脈;實驗3組:氪激光光凝Wistar大鼠全部視網膜分支靜脈;實驗4組:空白對照組(未經任何處理)。利用虹膜熒光造影(iris fluorescence angiography,IFA)對各實驗組大鼠進行鑒定,對NVI動物模型複製成功的大鼠採用Miller方法進行分級,對大鼠NVI形成前1天及模型複製後3、7、10、14、21、30 d分彆抽取房水,採用酶聯免疫吸附實驗(enzyme-linked immunosorbent assay,ELISA)檢測房水中VEGF、HGF的含量。採用SPSS 13.0統計軟件分析實驗組及對照組之間不同時間組房水中VEGF、HGF 含量變化,併分析兩種因子的相關性。結果單純應用孟加拉玫瑰紅尾部靜脈註射和單純激光的方法不能使大鼠產生視網膜缺血,用尾部靜脈註射孟加拉玫瑰紅光敏劑+氪激光光凝法可誘導大鼠視網膜靜脈阻塞,成功製作NVI動物模型。 NVI模型複製前1 d房水中VEGF和HGF的濃度分彆為(15.58±5.85)pg/ml和(22.84±6.75)pg/ml,複製後3、7、10、14、21、30 d二者濃度分彆為(17.30±4.80)、(31.83±9.90)、(36.89±11.73)、(57.34±18.55)、(112.03±46.41)、(106.93±52.89)pg/ml和(24.58±7.18)、(36.47±11.28)、(46.27±13.11)、(59.25±15.47)、(90.71±29.91)、(102.08±34.82)pg/ml;VEGF和HGF與NVI形成呈正相關,但二者濃度無相關性。結論大鼠虹膜新生血管的形成與其房水中VEGF、HGF含量成正相關,二者在虹膜新生血管形成中可能扮縯重要角色。
목적:탐토혈관내피생장인자(vascular endothelial growth factor,VEGF)、간세포생장인자(hepatocyte growth factor,HGF)재홍막신생혈관(neovascularization of the iris,NVI)대서모형방수중적함량변화。방법장청길급건강10주령Wistar대서수궤분위4조,매조20지(40안),을미흡입법전신마취대서,실험1조:경미부정맥주사맹가랍매괴홍광민제;실험2조:미부정맥주사맹가랍매괴홍광민제+극격광광응Wistar대서전부시망막분지정맥;실험3조:극격광광응Wistar대서전부시망막분지정맥;실험4조:공백대조조(미경임하처리)。이용홍막형광조영(iris fluorescence angiography,IFA)대각실험조대서진행감정,대NVI동물모형복제성공적대서채용Miller방법진행분급,대대서NVI형성전1천급모형복제후3、7、10、14、21、30 d분별추취방수,채용매련면역흡부실험(enzyme-linked immunosorbent assay,ELISA)검측방수중VEGF、HGF적함량。채용SPSS 13.0통계연건분석실험조급대조조지간불동시간조방수중VEGF、HGF 함량변화,병분석량충인자적상관성。결과단순응용맹가랍매괴홍미부정맥주사화단순격광적방법불능사대서산생시망막결혈,용미부정맥주사맹가랍매괴홍광민제+극격광광응법가유도대서시망막정맥조새,성공제작NVI동물모형。 NVI모형복제전1 d방수중VEGF화HGF적농도분별위(15.58±5.85)pg/ml화(22.84±6.75)pg/ml,복제후3、7、10、14、21、30 d이자농도분별위(17.30±4.80)、(31.83±9.90)、(36.89±11.73)、(57.34±18.55)、(112.03±46.41)、(106.93±52.89)pg/ml화(24.58±7.18)、(36.47±11.28)、(46.27±13.11)、(59.25±15.47)、(90.71±29.91)、(102.08±34.82)pg/ml;VEGF화HGF여NVI형성정정상관,단이자농도무상관성。결론대서홍막신생혈관적형성여기방수중VEGF、HGF함량성정상관,이자재홍막신생혈관형성중가능분연중요각색。
Objective To discuss the content variation of VEGF and HGF in aqueous humor in rats in iris neovascularization. Methods The eighty clean health level 10 weeks of Wistar rats were randomly divided into 4 groups and ether inhalation anesthesia. Groups 1: by tail intravenous injection of 3,4,5,6-Tetrachlorofluorescein;groups 2: tail intravenous injection of 3,4,5,6-Tetrachlorofluorescein+krypton laser photocoagulation in Wistar rats all branch retinal vein;group 3:krypton laser photocoagulation in Wistar rats all branch retinal vein;group 4:control group(without any treatment). The NVI success rat model identification was used iris fluorescence angiography technology. Then, the NVI success rat models were classed by Miller methods. The rat aqueous fluid was abstracted on 1 day before and after 3, 7, 10, 14, 21, 30 days model respectively. The concentration of VEGF and HGF of aqueous fluid were detected by enzyme-linked immunosorbent assay. The differences between the experimental data, the correlation analysis were completed by SPSS 13.0 software. Results Single application of Bangladesh rosy tail vein injection and single laser method didn’t cause retinal ischemia in rats, but the tail vein injection of Bangladesh rosy photosensitizer+krypton laser photocoagulation method could induced retinal vein occlusion in rats. It successfully produced NVI animal model. NVI model replication before 1 day of VEGF and HGF concentrations were (15.58±5.85) pg/ml and (22.84±6.75) pg/ml, respectively. And the VEGF and HGF concentrations after 7, 10, 3, 14, 21 30 days were (17.30±4.80), (31.83±9.90), (36.89±11.73), (57.34±18.55), (112.03±46.41), (106.93±52.89) pg/ml and (24.58±7.18), (36.47±11.28), (46.27±13.11), (59.25±15.47), (90.71±29.91), (102.08±34.82) pg/ml, respectively. VEGF and HGF and NVI formation were positively correlated, but aqueous HGF concentrations were unrelated to those of VEGF. Conclusion It presented positive correlation between the formation of neovascularization of the Iris in rats and VEGF, HGF content in aqueous humor. The VEGF and HGF played an important role in the iris neovascularization.