CAJ | 학술논문

目的：探讨佐剂性诱导骨关节炎大鼠模型血清和脊髓背根神经节中SP表达变化，及其与痛觉评分的相关性和塞来昔布的干预效应。方法将40只SD大鼠随机分为4组：A组（正常组10只）、B组（正常＋塞来昔布组10只）、C组（骨关节炎组10只）、D组（骨关节炎＋塞来昔布组10只），C和D组予以左膝和尾部皮内分别注射完全弗氏佐剂（CFA）诱导骨关节炎模型，通过酶联免疫吸附测定（ELISA）塞来昔布对于血浆和脊髓背根神经节内SP含量的影响，采用痛觉评分分析塞来昔布对骨关节炎后疼痛行为的影响。结果与对照组比较（A和B组），骨关节炎大鼠模型（C和D组）脊髓和脊髓背根神经节内SP含量明显升高（P＜0.01），且 SP 含量与疼痛疼痛评分呈正相关（r＝0.83，P＜0.01；r＝0.61，P＜0.01）；并且塞来昔布治疗组（D组）SP含量较C组显著降低（P＜0.05）。结论 SP在骨关节炎痛的发生中具有重要作用，塞来昔布抑制SP的合成或释放，从而缓解疼痛。
목적：탐토좌제성유도골관절염대서모형혈청화척수배근신경절중SP표체변화，급기여통각평분적상관성화새래석포적간예효응。방법장40지SD대서수궤분위4조：A조（정상조10지）、B조（정상＋새래석포조10지）、C조（골관절염조10지）、D조（골관절염＋새래석포조10지），C화D조여이좌슬화미부피내분별주사완전불씨좌제（CFA）유도골관절염모형，통과매련면역흡부측정（ELISA）새래석포대우혈장화척수배근신경절내SP함량적영향，채용통각평분분석새래석포대골관절염후동통행위적영향。결과여대조조비교（A화B조），골관절염대서모형（C화D조）척수화척수배근신경절내SP함량명현승고（P＜0.01），차 SP 함량여동통동통평분정정상관（r＝0.83，P＜0.01；r＝0.61，P＜0.01）；병차새래석포치료조（D조）SP함량교C조현저강저（P＜0.05）。결론 SP재골관절염통적발생중구유중요작용，새래석포억제SP적합성혹석방，종이완해동통。
Objective To explore the changes and significance of substance P in dorsal root ganglion and serum, analyze the correlation of substance P and pain score in complete Freund's adjuvant (CFA) induced osteoarthritis rat model, and to detect the effect of celecoxib on substance P and pain score. Methods Forty Sprague Dawley rats were randomly divided into four groups with 10 rats in each group:Group A (control), Group B (celecoxib), Group C (osteoarthritis), Group D (osteoarthritis+celecoxib). The osteoarthritis rat model in Group C and D were established by using an injection of complete Freund’s adjuvant intradermally at the base of the tail and another intra-articularly (left tibiofemoral). Contents of substance P in dorsal root ganglion and serum were measured by ELISA(enzyme-linked immunosorbent assay) and the pain were evaluated according to pain score system. Results Contents of substance P in osteoarthritis group (Group C and D) were significantly greater than those in control group (P<0.01);Substance P content in dorsal root ganglion and serum in osteoarthritis group was positively correlated with pain scores (r=0.83, P<0.01;r=0.61, P<0.01);The level of substance P content and pain scores were significantly decreased by celecoxib in Group D (P<0.05). Conclusion substance P plays an important role in occurrence of pain in osteoarthritis. Celecoxib can inhibit the production of substance P in serum and DRG and ameliorate pain.