中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
18期
8299-8303
,共5页
郑文莉%李贵平%黄宝丹%杜丽%黄凯
鄭文莉%李貴平%黃寶丹%杜麗%黃凱
정문리%리귀평%황보단%두려%황개
抗原,CD45%抗体,单克隆%放射性同位素%生物分布%188Re
抗原,CD45%抗體,單剋隆%放射性同位素%生物分佈%188Re
항원,CD45%항체,단극륭%방사성동위소%생물분포%188Re
Antigens,CD45%Antibodies,monoclonal%Radioisotopes%Biodistribution%Rhenium-188
目的:利用188Re直接法标记CD45单抗,探讨其在正常小鼠体内的生物学分布特性。方法应用2-巯基乙醇(2-ME)还原CD45单抗分子中的二硫键形成巯基;以氯化亚锡作为188Re的还原剂,葡庚糖酸钠为中间弱配体,188Re直接标记CD45单抗;PD-10层析柱分离纯化,纸层析法测定标记率与放化纯;鉴定188Re-CD45单抗的体外稳定性;研究188Re-CD45单抗在正常小鼠体内的生物分布特性。结果188Re-CD45单抗的标记率平均为(85.25±2.63)%,PD-10柱纯化后的放化纯为(92.54±3.56)%,比活度平均为(2.06±0.07)TBq/mmol;室温下放置24 h,188Re-CD45单抗放化纯为(64.33±1.53)%;在鼠血清和生理盐水中37℃下孵育24 h后,其放化纯仍有(64.2±3.77)%和(56.7±4.16)%。小鼠体内生物分布显示,188Re-CD45单抗主要分布于肾脏和肝脏,其次是肺脏、骨骼和血液。结论188Re直接法标记CD45单抗的方法简单易行,标记率较高,具有良好的体外稳定性;188Re-CD45单抗静脉注射后,体内放射性主要经肾脏排泄,并在肝脏有较高的浓聚,符合标记抗体的体内分布规律。
目的:利用188Re直接法標記CD45單抗,探討其在正常小鼠體內的生物學分佈特性。方法應用2-巰基乙醇(2-ME)還原CD45單抗分子中的二硫鍵形成巰基;以氯化亞錫作為188Re的還原劑,葡庚糖痠鈉為中間弱配體,188Re直接標記CD45單抗;PD-10層析柱分離純化,紙層析法測定標記率與放化純;鑒定188Re-CD45單抗的體外穩定性;研究188Re-CD45單抗在正常小鼠體內的生物分佈特性。結果188Re-CD45單抗的標記率平均為(85.25±2.63)%,PD-10柱純化後的放化純為(92.54±3.56)%,比活度平均為(2.06±0.07)TBq/mmol;室溫下放置24 h,188Re-CD45單抗放化純為(64.33±1.53)%;在鼠血清和生理鹽水中37℃下孵育24 h後,其放化純仍有(64.2±3.77)%和(56.7±4.16)%。小鼠體內生物分佈顯示,188Re-CD45單抗主要分佈于腎髒和肝髒,其次是肺髒、骨骼和血液。結論188Re直接法標記CD45單抗的方法簡單易行,標記率較高,具有良好的體外穩定性;188Re-CD45單抗靜脈註射後,體內放射性主要經腎髒排洩,併在肝髒有較高的濃聚,符閤標記抗體的體內分佈規律。
목적:이용188Re직접법표기CD45단항,탐토기재정상소서체내적생물학분포특성。방법응용2-구기을순(2-ME)환원CD45단항분자중적이류건형성구기;이록화아석작위188Re적환원제,포경당산납위중간약배체,188Re직접표기CD45단항;PD-10층석주분리순화,지층석법측정표기솔여방화순;감정188Re-CD45단항적체외은정성;연구188Re-CD45단항재정상소서체내적생물분포특성。결과188Re-CD45단항적표기솔평균위(85.25±2.63)%,PD-10주순화후적방화순위(92.54±3.56)%,비활도평균위(2.06±0.07)TBq/mmol;실온하방치24 h,188Re-CD45단항방화순위(64.33±1.53)%;재서혈청화생리염수중37℃하부육24 h후,기방화순잉유(64.2±3.77)%화(56.7±4.16)%。소서체내생물분포현시,188Re-CD45단항주요분포우신장화간장,기차시폐장、골격화혈액。결론188Re직접법표기CD45단항적방법간단역행,표기솔교고,구유량호적체외은정성;188Re-CD45단항정맥주사후,체내방사성주요경신장배설,병재간장유교고적농취,부합표기항체적체내분포규률。
Objective With direct-labeling method of CD45 McAb with 188Re, to investigate its bio-distribution character in normal mice. Methods The disulfide bond in the molecule of CD45 monoclonal antibody was reduced to form a mercapto group by the mercaptoethanol (2-ME). The labeling was conducted by stannous chloride used as reductant of 188Re, and sodium glucoheptonate as intermediate.weak ligands, then 188Re was directly labeled CD45 mAb alone; The reaction mixture was separated and purified throuth the PD-10 column;Labeling efficiency and radiochemical purity were measured by the paper chromatography. Then stability of 188Re labeled CD45 McAb was determined in vitro. The biodistribution in the healthy Kunming mice after intravenous injection of 188Re-CD45 McAb was determined. Results The labeling efficiency of 188Re-CD45 McAb was (85.25±2.63)%, and radiochemical purity was (92.54±3.56)%. The specific activity was (2.06±0.07) TBq/mmol;The radiochemical purity of 188Re-CD45 McAb was (64.33±1.53)% after incubating 24 h in room temperature. While mixed the saline and healthy rat serum at 37℃for 24 h, the radiochemical purity was (64. 2±3. 77)%and(56. 7±4. 16)%, respectively. The biodistribution result showed that the radioactivity in body was mainly distributed in kidney and liver, followed by lung, bone and blood. Conclusion The method of direct-labeling CD45 McAb with 188Re is not only simple, but also has high labeling efficiency. 188Re-CD45 McAb has good stability in vitro. After injected intravenously, radioactive label is mainly excreted through kidneys with a higher accumulation in liver, and it accords with the in vivo kinetics characteristic of labeled antibody.