CAJ | 학술논문

目的：研究T型分叉血管对RGD肽对内皮细胞（endothelial cell，EC）在涂层材料聚乙二醇-聚乳酸-聚谷氨酸共聚物（PEG-PLA-PGL）表面黏附稳定性的影响。为该材料作为冠状动脉内支架涂层材料的临床应用提供实验依据。方法实验材料（PEG-PLA-PGL）分为两组：RGD组（表面共价接枝人工合成的RGD三肽）、对照组（共聚物组），然后在两组材料表面种植体外培养的人脐静脉EC，并在固定常流条件下观察比较在T型管不同位置、不同流速下RGD肽对材料表面细胞黏附稳定性的影响。结果作用4 h后，在共聚物组及RGD组T型管分叉前（A）、分叉后（B）、分叉（C）三处位置上EC细胞残余率在最大流速（0.8 m/s）下分别为（14.98±1.45）%vs.（17.38±2.52）%、（10.06±2.12）%vs.（14.25±2.03）%、（11.08±2.18）%vs.(13.27±1.89）%。发现三组存在明显统计学差异（P＜0.05）。共聚物表面结合RGD后，细胞残余率明显增加。但是无论在对照组还是在RGD组，T型分叉前A位置EC细胞残余率都要比分叉及分叉处的细胞残余率高（P＜0.05）。结论 RGD可以提高EC在共聚物材料表面的黏附稳定性，但是T型分叉对EC的黏附稳定性有一定的影响。
목적：연구T형분차혈관대RGD태대내피세포（endothelial cell，EC）재도층재료취을이순-취유산-취곡안산공취물（PEG-PLA-PGL）표면점부은정성적영향。위해재료작위관상동맥내지가도층재료적림상응용제공실험의거。방법실험재료（PEG-PLA-PGL）분위량조：RGD조（표면공개접지인공합성적RGD삼태）、대조조（공취물조），연후재량조재료표면충식체외배양적인제정맥EC，병재고정상류조건하관찰비교재T형관불동위치、불동류속하RGD태대재료표면세포점부은정성적영향。결과작용4 h후，재공취물조급RGD조T형관분차전（A）、분차후（B）、분차（C）삼처위치상EC세포잔여솔재최대류속（0.8 m/s）하분별위（14.98±1.45）%vs.（17.38±2.52）%、（10.06±2.12）%vs.（14.25±2.03）%、（11.08±2.18）%vs.(13.27±1.89）%。발현삼조존재명현통계학차이（P＜0.05）。공취물표면결합RGD후，세포잔여솔명현증가。단시무론재대조조환시재RGD조，T형분차전A위치EC세포잔여솔도요비분차급분차처적세포잔여솔고（P＜0.05）。결론 RGD가이제고EC재공취물재료표면적점부은정성，단시T형분차대EC적점부은정성유일정적영향。
Objective Study the effect of RGD Peptide on Adhesive Stability of Human Umbilical Vein Endothelial Cell on PEG-PLA-PGL copolymer surface in the T-shape Bifurcation to provide experimental evidence for for RGD as the coating material of coronary stent in clinical application. Methods PEG-PLA-PGL copolymer were divided into two groups:RGD group (covalently grafting synthetic tripeptide RGD) and control group (copolymer), and the 2 groups of implants surface cultured human umbilical vein endothelial cells, and in steady flow conditions observe and compare of different locations, different flow rates RGD peptide fixed in the T-tube on the surface of cell adhesion stability. Results After 4 h action, the control groups and the RGD groups [in the T-tube at the position A (front fork), B (after bifurcation), C (bifurcation)] under maximum flow rate (0.8 m/s), the EC residual rate respectively for the 14.98%±1.45% vs. 17.38%±2.52%, 10.06%±2.12% vs. 14.25%±2.03%, 11.08%±2.18%vs. 13.27%±1.89%. Found that three groups exist significant difference (P<0.05). In the RGD groups, the EC residual rate was more compared with the control groups. However, both in the control groups and the RGD groups, T-type front fork position A EC residual ratio better than the position B and C (P<0.05). Conclusion RGD can improve endothelial cell adhesion stability in the copolymer material surface, but the T-shaped bifurcation do has a certain impact on the stability adhesion of the EC.