CAJ | 학술논문

目的：研究冠状动脉支架材料表面RGD修饰PEG-PLA-PGL共聚物涂层体外血液相容性，为该聚合物作为冠状动脉内支架涂层材料的临床应用提供实验依据。方法以裸钢片、PEG-PLA-PGL聚合物涂层钢片为对照组，通过体外溶血实验、血小板吸附数量测定、抗凝血时间测定及蛋白吸附评价RGD修饰PEG-PLA-PGL共聚物（PEG-PLA-PGL/RGD）涂层钢片的体外血液相容性。结果裸钢片、PEG-PLA-PGL组、PEG-PLA-PGL/RGD组三组钢片溶血率均＜5%，无溶血作用；与标本血比较，三组钢片APTT均表现出明显差异（P＝0.047、0.044、0.036），均能改善标本血抗凝血性能；PEG-PLA-PGL涂层与裸钢片相比，在各项所测指标中未表现出明显差异；接枝 RGD 后 PEG-PLA-PGL/RGD 涂层在血小板吸附及白蛋白吸附方面优于裸钢片，有统计学差异（P＝0.046、0.043），一定程度上提高了裸钢片的血液相容性。结论与裸钢片相比，RGD在聚合物表面的接枝提高了其血液相容性，PEG-PLA-PGL/RGD有望成为广阔发展前景的EPC捕获支架生物活性涂层材料。
목적：연구관상동맥지가재료표면RGD수식PEG-PLA-PGL공취물도층체외혈액상용성，위해취합물작위관상동맥내지가도층재료적림상응용제공실험의거。방법이라강편、PEG-PLA-PGL취합물도층강편위대조조，통과체외용혈실험、혈소판흡부수량측정、항응혈시간측정급단백흡부평개RGD수식PEG-PLA-PGL공취물（PEG-PLA-PGL/RGD）도층강편적체외혈액상용성。결과라강편、PEG-PLA-PGL조、PEG-PLA-PGL/RGD조삼조강편용혈솔균＜5%，무용혈작용；여표본혈비교，삼조강편APTT균표현출명현차이（P＝0.047、0.044、0.036），균능개선표본혈항응혈성능；PEG-PLA-PGL도층여라강편상비，재각항소측지표중미표현출명현차이；접지 RGD 후 PEG-PLA-PGL/RGD 도층재혈소판흡부급백단백흡부방면우우라강편，유통계학차이（P＝0.046、0.043），일정정도상제고료라강편적혈액상용성。결론여라강편상비，RGD재취합물표면적접지제고료기혈액상용성，PEG-PLA-PGL/RGD유망성위엄활발전전경적EPC포획지가생물활성도층재료。
Objective To investigate the blood compatibility of PEG-PLA-PGL/RGD on 316L stainless steel surface in vitro so as to provide further experimental reference for its clinical application. Methods The blood compatibility was evaluated by hemolysis assay, platelet adhesion experiments, anticoagulant time measurement and protein adsorption test. Results The hemolysis rate of each group was less than 5%, which suggested they were all avirulent;APTT remarkable prolonged compared with plasma sample, showed anti-coagulant property of steel in three groups(P=0.047, 0.044, 0.036). Compared with bare steel, PEG-PLA-PGL steel had no apparent differences in any tests, meanwhile, PEG-PLA-PGL/RGD steel adsorbed less platelets but larger amount of albumin(P=0.046, 0.043). Conclusion Blood compatibility of PEG-PLA-PGL/RGD steel has been improved compared with PEG-PLA-PGL steel and bare steel. PEG-PLA-PGL/RGD might be a potential appropriate material for coronary stent.