CAJ | 학술논문

目的：建立高效液相色谱法测定维生素B1片中维生素B1的含量。方法采用色谱柱：Diamonsil C18（150mm×4.6mm，5μm），以碳十八烷基键合硅胶为填充剂。流动相：甲醇-乙腈-0.02mol/L庚烷磺酸钠溶液（含1%三乙胺，用磷酸调节pH值至5.5，9∶9∶82）；检测波长为238nm；流速为1.0ml/min；进样量：10μl。结果维生素B1质量浓度在50.5～505μg/ml范围内与峰面积呈良好的线性关系（r＝0.99999，n＝5），平均回收率为100.4%，RSD＝0.13%。结论本方法简单、精确、可靠，可作为维生素B1片的质量控制方法。
목적：건립고효액상색보법측정유생소B1편중유생소B1적함량。방법채용색보주：Diamonsil C18（150mm×4.6mm，5μm），이탄십팔완기건합규효위전충제。류동상：갑순-을정-0.02mol/L경완광산납용액（함1%삼을알，용린산조절pH치지5.5，9∶9∶82）；검측파장위238nm；류속위1.0ml/min；진양량：10μl。결과유생소B1질량농도재50.5～505μg/ml범위내여봉면적정량호적선성관계（r＝0.99999，n＝5），평균회수솔위100.4%，RSD＝0.13%。결론본방법간단、정학、가고，가작위유생소B1편적질량공제방법。
Objective To establish HPLC method for the determination of Vitamin B1 Tablets.Methods Using Column:Diamonsil C18(150mm×4.6mm,5μm),Mobile phase:methanol-acetonitrile-0.02mol/L sodium heptane solution (containing 1%triethylamine,pH value adjusted to 5.5 with phosphoric acid,9∶9∶82).The flow rate was 1.0ml/min.The detection was carried out with an ultraviolet detector at 238nm.Thiamine hydrochloride was measured with the external standard method.Results The calibration curve was linear in the range of 50.5~505μg/ml.(r=0.99999,n=5),the average recovery rate was 100.4%,RSD=0.13%.Conclusion This method is simple,convenient,accurate and reliable.It can be used to control the quality of Vitamin B1 Tablets.