中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2013年
10期
1091-1094
,共4页
侯向生%王玉香%郑慧禹%赵冬峰
侯嚮生%王玉香%鄭慧禹%趙鼕峰
후향생%왕옥향%정혜우%조동봉
食管癌%P53%Ki67%检测
食管癌%P53%Ki67%檢測
식관암%P53%Ki67%검측
Esophageal%cancer%P53%Ki67%Detect
目的 探讨P53、Ki67的表达在食管癌细胞增殖、侵袭和转移中的临床意义及相互关系.方法 随机收取2006年1月至2008年5月食管癌切除标本并脉管瘤栓86例,作为瘤栓组.并随机抽取食管癌无瘤栓86例作为对照组.共计172例进行研究.应用免疫组化二步法检测172例食管癌患者P53、Ki67的表达.结果 P53表达率在瘤栓组和对照组分别为66.3％ (57/86)和51.1％ (44/86),差异有统计学意义(x2 =4.045,P＜0.05),≥++阳性表达差异有统计学意义(x2=8.592,P＜0.05)；Ki67表达率在瘤栓组和对照组分别为76.7％(66/86)和60.5％(82/86),差异有统计学意义(x2 =5,291,P＜0.05),≥++阳性表达差异有统计学意义(x2=13.661,P＜0.01)；P53与Ki67在两组标本中的表达呈正相关(r =0.400,P=0.00).瘤栓组P53、Ki67表达与癌细胞浸润深度(x2值分别为4.923、5.977,P均＜0.05)、淋巴结转移(x2值分别为3.945、5.794,P均＜0.05)、癌细胞分化程度(x2值分别为5.570、4.354,P均＜0.05)有关.结论 P53、Ki67的过度表达在食管癌细胞的增殖、侵袭和转移中起一定作用,共同影响着食管癌的发生、发展过程,两者联合检测有助于食管癌的早期诊断,并可判断其恶性程度、侵袭、转移及预后.
目的 探討P53、Ki67的錶達在食管癌細胞增殖、侵襲和轉移中的臨床意義及相互關繫.方法 隨機收取2006年1月至2008年5月食管癌切除標本併脈管瘤栓86例,作為瘤栓組.併隨機抽取食管癌無瘤栓86例作為對照組.共計172例進行研究.應用免疫組化二步法檢測172例食管癌患者P53、Ki67的錶達.結果 P53錶達率在瘤栓組和對照組分彆為66.3％ (57/86)和51.1％ (44/86),差異有統計學意義(x2 =4.045,P＜0.05),≥++暘性錶達差異有統計學意義(x2=8.592,P＜0.05)；Ki67錶達率在瘤栓組和對照組分彆為76.7％(66/86)和60.5％(82/86),差異有統計學意義(x2 =5,291,P＜0.05),≥++暘性錶達差異有統計學意義(x2=13.661,P＜0.01)；P53與Ki67在兩組標本中的錶達呈正相關(r =0.400,P=0.00).瘤栓組P53、Ki67錶達與癌細胞浸潤深度(x2值分彆為4.923、5.977,P均＜0.05)、淋巴結轉移(x2值分彆為3.945、5.794,P均＜0.05)、癌細胞分化程度(x2值分彆為5.570、4.354,P均＜0.05)有關.結論 P53、Ki67的過度錶達在食管癌細胞的增殖、侵襲和轉移中起一定作用,共同影響著食管癌的髮生、髮展過程,兩者聯閤檢測有助于食管癌的早期診斷,併可判斷其噁性程度、侵襲、轉移及預後.
목적 탐토P53、Ki67적표체재식관암세포증식、침습화전이중적림상의의급상호관계.방법 수궤수취2006년1월지2008년5월식관암절제표본병맥관류전86례,작위류전조.병수궤추취식관암무류전86례작위대조조.공계172례진행연구.응용면역조화이보법검측172례식관암환자P53、Ki67적표체.결과 P53표체솔재류전조화대조조분별위66.3％ (57/86)화51.1％ (44/86),차이유통계학의의(x2 =4.045,P＜0.05),≥++양성표체차이유통계학의의(x2=8.592,P＜0.05)；Ki67표체솔재류전조화대조조분별위76.7％(66/86)화60.5％(82/86),차이유통계학의의(x2 =5,291,P＜0.05),≥++양성표체차이유통계학의의(x2=13.661,P＜0.01)；P53여Ki67재량조표본중적표체정정상관(r =0.400,P=0.00).류전조P53、Ki67표체여암세포침윤심도(x2치분별위4.923、5.977,P균＜0.05)、림파결전이(x2치분별위3.945、5.794,P균＜0.05)、암세포분화정도(x2치분별위5.570、4.354,P균＜0.05)유관.결론 P53、Ki67적과도표체재식관암세포적증식、침습화전이중기일정작용,공동영향착식관암적발생、발전과정,량자연합검측유조우식관암적조기진단,병가판단기악성정도、침습、전이급예후.
Objective To investigate the clinical significance of expression of P53,Ki67 in proliferation,invasion and metastasis of cancer cells of patients with esophageal carcinoma.Methods Eighty-six patients with esophageal carcinoma were selected as tumor group from January 2006 to May 2008.And another 86 patients without tumor were as control group.ABC-immunohistochemistry was used to detect the expression of P53 and Ki67 in 172 cases of patients with esophageal carcinoma.Results The expression of P53 in patients with esophageal carcinoma was different from that in control subjects (x2 =4.045,P ＜ 0.05),and the P53 expression rate in two group were 66.3％ (57/86) and 51.1％ (44/86) respectively.The rate of P53 expression reaching to + + in two groups was also significant (x2 =8.592,P ＜ 0.01).Ki67 expression in patients with esophageal carcinoma was higher than that of control patients (76.7％ (66/86) vs.60.5％ (82/86),x2 =5.291,P ＜ 0.05).Additionally,there was significant difference regarding of Ki6 expression reaching to + + positive(x2 =13.661,P ＜ 0.01).Moreover,the expression of P53 and Ki67 were found to be a positively correlation(r =0.400,P =0.00).The expression of P53 and Ki67 were related with invasion and differentiation of cancer cells (x2 =3.945,5.794 respectively,all P ＜ 0.05) and lymph node metastasis (x2 =5.570,4.354 respectively,all P ＜ 0.05).Conclusion Over-expression of P53 and Ki67 were involved in the process of proliferation,invasion and metastasis of cancer cells in esophageal carcinoma,which coherently impacted the occurrence and development of esophageal cancer.The combined detection of P53 and Ki67 seems to help the early diagnosis of esophageal cancer and evaluation of the degree of malignancy,invasion,metastasis and prognosis.Over-expression of P53,Ki67 might be served as a reference marker in screening for chemotherapy for esophageal cancer.
