CAJ | 학술논문

目的：探讨黄芪与不同剂量柴胡配伍对脾虚发热大鼠模型的退热作用及机制。方法：雄性Wistar大鼠随机分为正常对照组、脾虚发热组、芪柴大、中、小剂量组、柴胡组共6组。采用饮食失节＋游泳疲劳＋LPS制备大鼠脾虚发热模型。实验第17 d开始，各给药组大鼠分别按剂量4.56 g/kg、2.95 g/kg、2.41 g/kg、2.41 g/kg给予灌胃，正常组和脾虚发热组给予同体积蒸馏水，每日1次，连续6 d。第22天模型组和各给药组ip LPS（80μg/kg），正常对照组ip等量生理盐水。观测各组大鼠ip后不同时间点的体温，测定血中细胞因子和下丘脑体温调节递质的含量。结果：与正常组相比，脾虚发热组大鼠在ip LPS后各点体温均见不同程度升高，60 min和220 min时升高明显（P＜0.05）；血IL-2显著升高（P＜0.05）；下丘脑cAMP和PGE2显著升高（P＜0.05），AVP呈下降趋势。与脾虚发热模型组相比，各给药组大鼠体温均出现不同程度下降（P＜0.05），下丘脑PGE2或AVP呈现降低或升高趋势，其中柴胡组和芪柴小剂量组的体温下降明显，下丘脑IL-1β显著降低（P＜0.05），芪柴大剂量组血IL-1β显著升高（P＜0.05）。与芪柴大剂量组比，芪柴中、小剂量组及柴胡组血IL-1β均显著降低（P＜0.05），芪柴中、小剂量组血IL-2显著升高（ P＜0.05）。结论：黄芪配伍柴胡不同剂量及单用大剂柴胡对脾虚发热模型均有不同程度的退热作用，其退热机制可能与降低中枢发热递质PGE2和升高AVP有关，黄芪与小剂柴胡配伍和单纯大剂柴胡的退热作用可能还涉及到中枢IL-1β的降低。
목적：탐토황기여불동제량시호배오대비허발열대서모형적퇴열작용급궤제。방법：웅성Wistar대서수궤분위정상대조조、비허발열조、기시대、중、소제량조、시호조공6조。채용음식실절＋유영피로＋LPS제비대서비허발열모형。실험제17 d개시，각급약조대서분별안제량4.56 g/kg、2.95 g/kg、2.41 g/kg、2.41 g/kg급여관위，정상조화비허발열조급여동체적증류수，매일1차，련속6 d。제22천모형조화각급약조ip LPS（80μg/kg），정상대조조ip등량생리염수。관측각조대서ip후불동시간점적체온，측정혈중세포인자화하구뇌체온조절체질적함량。결과：여정상조상비，비허발열조대서재ip LPS후각점체온균견불동정도승고，60 min화220 min시승고명현（P＜0.05）；혈IL-2현저승고（P＜0.05）；하구뇌cAMP화PGE2현저승고（P＜0.05），AVP정하강추세。여비허발열모형조상비，각급약조대서체온균출현불동정도하강（P＜0.05），하구뇌PGE2혹AVP정현강저혹승고추세，기중시호조화기시소제량조적체온하강명현，하구뇌IL-1β현저강저（P＜0.05），기시대제량조혈IL-1β현저승고（P＜0.05）。여기시대제량조비，기시중、소제량조급시호조혈IL-1β균현저강저（P＜0.05），기시중、소제량조혈IL-2현저승고（ P＜0.05）。결론：황기배오시호불동제량급단용대제시호대비허발열모형균유불동정도적퇴열작용，기퇴열궤제가능여강저중추발열체질PGE2화승고AVP유관，황기여소제시호배오화단순대제시호적퇴열작용가능환섭급도중추IL-1β적강저。
Objective:To explore the antipyretic action and mechanism of Astragalus complying with different dosages of Radix Bupleuri on the rat models of spleen-qi deficiency with fever .Methods: The male wistar rats were randomly assigned to the normal group , the spleen-qi deficiency with fever group , the astragalus with large dosage radix bupleuri group , the astragalus with medium dosage radix bu-pleuri group , the astragalus with small dosage radix bupleuri group , and the radix bupleuri group .The model rats of spleen-qi deficiency were prepared through excess fatigue and out of constant diet for 21 days.The medicine group rats were fed with 4.56g/kg (large), 2. 95g/kg (medium) 2.41g/kg (small) and 2.41g/kg (radix bupleuri only) once a day and the other two groups were given physiological saline from the seventeenth day .At 8:00 am on the 22th day,the rats of model group and medicine groups were respectively intraperito-nealy injected with lipopoly saccharide ( LPS, 8 0μg/kg) to induce fever.The rats of the normal group were given physiological saline . The rectal temperature was measured at different time after ip LPS .And then the amounts of cytokines in blood and transmitters which regulate temperature in hypothalamus were also measured .Results: Compared with the normal group , the temperature of rats in the spleen-qi deficiency with fever group raised to some extent at every observation time after ip LPS , especially at 60min and 220min(P<0.05).IL-2 in blood、CAMP and PGE2 in hypothalamus increased significantly(P<0.05).AVP in hypothalamus had decreased.Com-pared with the spleen-qi deficiency with fever group , the temperature of rats in every medicine group decreased significantly to some ex-tent(P<0.05).CAMP and PGE2 in hypothalamus increased or decreased (P<0.05).The temperature declined and IL-1βin hypothal-amus decreased significantly(P<0.05)in the radix bupleuri group and the astragalus with small dosage radix bupleuri group .Blood of IL-1βincreased significantly ( P<0.05) in the astragalus with large dosage radix bupleuri group .Compared with the astragalus with large dosage radix bupleuri group, blood IL-1βdecreased significantly(P<0.05)in the astragalus with medium and with small dosage radix bupleuri groups, as well as the radix bupleuri group.Blood IL-2 increased significantly(P<0.05)in the astragalus with medium and with small dosage radix bupleuri groups .Conclusion:Astragalus complying with different dosage of radix bupleuri and only application of large dosage of radix bupleuri had antipyretic effect to the model rat of spleen -qi deficiency with fever .Its antipyretic mechanism may be connected with reducing central pyrexia amboceptor PGE 2 and increasing AVP .And astragalus complying with small dosage of radix bupleuri and the only application of large dosage radix bupleuri may be also related to declining of central IL -1β.