CAJ | 학술논문

目的研究CC趋化因子受体-2(CC chemokine receptor 2,CCR-2)在肝细胞癌组织中的表达及其与肝癌临床病理特征、预后的关系.方法用免疫组织化学法(SP法)检测76例肝癌组织及其癌旁组织中CCR-2的表达情况,分析CCR-2表达与肝癌各项临床病理特征以及患者预后的关系.CCR-2染色结果与各项临床指标的关系采用x2检验或Fisher确切概率法检验.采用KaplanMeier法绘制生存曲线.生存情况分析采用log-rank检验.结果 14(18.4％)例癌旁组织CCR-2染色结果显示为弱阳性表达,其余均为阴性.在肝癌组织中,CCR-2表达明显高于癌旁组织:15(19.7％)例阴性表达,29(38.2％)例弱阳性表达,12(15.8％)例中等阳性表达,20(26.3％)例强阳性表达.肝癌组织中CCR-2阳性表达与肿瘤直径(X2=12.41,P＜0.01)、门静脉癌栓(x2=7.476,P=0.006)、转移(X2=7.227,P=0.007)、AJCC分期(X2=20.711,P＜0.01)明显相关.CCR-2高表达组患者术后五年生存率(6.3％)明显低于低表达组(38.6％),差异具有统计学意义(X2 =27.133,P＜0.01).经单因素及多因素分析显示,肝癌组织CCR-2高表达是影响肝癌患者总体生存的独立危险因素(P＜0.05).结论 CCR-2在肝癌组织中表达明显升高,可能参与了肝癌的发生发展过程,检测CCR-2表达水平对判断肝癌的预后具有一定的参考价值.
목적 연구CC추화인자수체-2(CC chemokine receptor 2,CCR-2)재간세포암조직중적표체급기여간암림상병리특정、예후적관계.방법 용면역조직화학법(SP법)검측76례간암조직급기암방조직중CCR-2적표체정황,분석CCR-2표체여간암각항림상병리특정이급환자예후적관계.CCR-2염색결과여각항림상지표적관계채용x2검험혹Fisher학절개솔법검험.채용KaplanMeier법회제생존곡선.생존정황분석채용log-rank검험.결과 14(18.4％)례암방조직CCR-2염색결과현시위약양성표체,기여균위음성.재간암조직중,CCR-2표체명현고우암방조직:15(19.7％)례음성표체,29(38.2％)례약양성표체,12(15.8％)례중등양성표체,20(26.3％)례강양성표체.간암조직중CCR-2양성표체여종류직경(X2=12.41,P＜0.01)、문정맥암전(x2=7.476,P=0.006)、전이(X2=7.227,P=0.007)、AJCC분기(X2=20.711,P＜0.01)명현상관.CCR-2고표체조환자술후오년생존솔(6.3％)명현저우저표체조(38.6％),차이구유통계학의의(X2 =27.133,P＜0.01).경단인소급다인소분석현시,간암조직CCR-2고표체시영향간암환자총체생존적독립위험인소(P＜0.05).결론 CCR-2재간암조직중표체명현승고,가능삼여료간암적발생발전과정,검측CCR-2표체수평대판단간암적예후구유일정적삼고개치.
Objective To investigate the expression of CC chemokine receptor 2 (CCR-2) in hepatocellular carcinoma (HCC) and to evaluate its relationship with the clinicopathological features and prognosis of HCC.Methods Immunohistochemistry was used to detect the expression of CCR-2 in the HCC tissues and their corresponding non-cancerous adjacent liver tissues in 76 patients with HCC.Statistical analysis was used to determine the association of CCR-2 expression with clinicopathological features and prognosis.The expressions of CCR-2 and the relationship between CCR-2 expression and clinicopathological features and prognosis were analyzed using the Chi-square test and Fisher exact probability test.The survival curve was drawn using the Kaplan-Meier method,and the survival was analyzed using the log-rank test.Results Weak positive staining of CCR-2 was detected in the non-cancerous adjacent liver tissues of 14 samples (18.4％) and negative staining was detected in the non-cancerous adjacent liver tissues of the remaining samples.The expression of CCR-2 was different in the HCC tissues:negative CCR-2 was detected in 15(19.7％) samples,weak positive staining of CCR-2 in 29(38.2％) samples,moderate positive staining of CCR-2 in 12(15.8％) samples,and strong positive staining of CCR-2 in 20(26.3％) samples.The high expression of CCR-2 strongly correlated with tumor size (x2 =12.41,P＜0.01),venous invasion (x2 =7.476,P=0.006),metastasis (x2=7.227,P=0.007) and AJCC TNM stage (x2=20.711,P＜0.01).The cumulative 5-year survival rate was 38.6％ in the low CCR-2 expression group,whereas it was 6.3％ in the high CCR-2 expression group (x2=27.133,P＜0.01).Univariate analysis and multivariate analysis showed that high CCR-2 expression was an independent predictor of overall survival of HCC (P＜0.05).Conclusions The expression of CCR-2 is highly up-regulated in HCC tissues,indicating that high CCR-2 expression is involved in the process of HCC carcinogenesis and progression,and its expression may serve as a biomarker for predicting prognosis of patients with HCC.