CAJ | 학술논문

背景：双膦酸盐可以提高骨密度、抑制骨吸收的作用已被临床所证实，但其对于骨骼基质结构的影响研究较少。 　　目的：实验通过观察双膦酸盐类药物--阿仑膦酸钠对骨结构及骨基质代谢的影响，探讨阿仑膦酸钠改善骨质量、提高骨强度的骨基质调控机制。 　　方法：实验建立去卵巢大鼠模型，用阿仑膦酸钠进行干预，同时设置模型组和假手术组进行对照。运用骨骼影像学、骨组织病理学和骨生物力学检测技术与酶联免疫吸附法观察阿仑膦酸钠对骨丢失大鼠骨密度、骨代谢、骨生物力学性能和骨结构的影响。 　　结果与结论：药物干预后4，8，12周，阿仑膦酸钠组骨密度均高于模型组(P<0.05)；药物干预8周后，与模型组相比，阿仑膦酸钠组骨代谢指标尿脱氧吡啶诺啉，血清Ⅰ型胶原羧基端前肽水平均降低(P<0.05)，腰椎和股骨的最大载荷、最大压强、模量以及Ⅰ型胶原不同交联形式的尿吡啶啉/脱氧吡啶啉值均增高(P<0.05)。结果证实，阿仑膦酸钠能够对抗因雌激素缺乏导致大鼠骨量的丢失，提高生物力学性能，改善骨基质结构，同时恢复因去卵巢所导致的Ⅰ型胶原交联组分的改变。
배경：쌍련산염가이제고골밀도、억제골흡수적작용이피림상소증실，단기대우골격기질결구적영향연구교소。 　　목적：실험통과관찰쌍련산염류약물--아륜련산납대골결구급골기질대사적영향，탐토아륜련산납개선골질량、제고골강도적골기질조공궤제。 　　방법：실험건립거란소대서모형，용아륜련산납진행간예，동시설치모형조화가수술조진행대조。운용골격영상학、골조직병이학화골생물역학검측기술여매련면역흡부법관찰아륜련산납대골주실대서골밀도、골대사、골생물역학성능화골결구적영향。 　　결과여결론：약물간예후4，8，12주，아륜련산납조골밀도균고우모형조(P<0.05)；약물간예8주후，여모형조상비，아륜련산납조골대사지표뇨탈양필정낙람，혈청Ⅰ형효원최기단전태수평균강저(P<0.05)，요추화고골적최대재하、최대압강、모량이급Ⅰ형효원불동교련형식적뇨필정람/탈양필정람치균증고(P<0.05)。결과증실，아륜련산납능구대항인자격소결핍도치대서골량적주실，제고생물역학성능，개선골기질결구，동시회복인거란소소도치적Ⅰ형효원교련조분적개변。
BACKGROUND:Bisphosphonates that can increase bone density and inhibit bone resorption have been clinical y confirmed, but the structure of the bone matrix has been less studied. OBJECTIVE:To observe the effects of alendronate on bone structure and bone matrix metabolism, and then to investigate the control ing mechanism by which alendronate improves bone mass and increase bone intensity. METHODS:An ovariectomized rat model was prepared and intervened with alendronate as treatment group. Model and sham-surgery groups were set as controls. Alendronate effects on bone mineral density, bone metabolism, bone biomechanics, and bone structure were observed in bone loss rats using bone imaging, bone tissue pathology and biomechanical test and enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION:Alendronate intervention could fight against bone loss as compared with model group at weeks 4, 8, and 12 after treatment (P<0.05). Compared with the model group, the expression of urinary deoxypyridinoline and serum carboxyterminal propeptide of type Ⅰ procol agen was decreased significantly after alendronate intervention (P<0.05);the maximal load, maximal pressure and modelus on the lumbar vertebrae and femur were increased as wel as ratio of urinary pyridinoline/deoxypyridinoline of type Ⅰ procol agen (P<0.05). These findings suggest that alendronate intervention can inhibit bone loss in rats induced by estrogen deficiency, increase biomechanical properties, improve bone matrix structure, and meanwhile, recover the Ⅰ col agen crosslinking component due to ovariectomy.