CAJ | 학술논문

背景：白血病侧群细胞表型的研究对于理解肿瘤细胞的异质性和起源、分子标记和靶向治疗等都有积极意义。 　　目的：鉴定人慢性粒细胞白血病细胞株 K562中是否存在侧群细胞，并观察侧群细胞亚群与非侧群细胞亚群中部分白细胞分化抗原的表达差异。 　　方法：采用流式细胞术检测K562细胞株中是否存在侧群细胞；并进一步分析K562侧群细胞和非侧群细胞两亚群间CD34+、CD34+CD38-、CD34+CD38+、HLA-DR+细胞的表达情况。 　　结果与结论：经Hoechst33342染色，流式细胞仪分析结果显示在K562中存在侧群细胞，这部分细胞比例少，为(2.7±0.5)%；统计学分析侧群细胞和非侧群细胞亚群中 CD34+、CD34+CD38-细胞表达率差异有显著性意义，而CD34+CD38+细胞表达率和HLA-DR+细胞表达率差异均无显著性意义；侧群细胞和非侧群细胞相比在分化抗原表达上有异质性。
배경：백혈병측군세포표형적연구대우리해종류세포적이질성화기원、분자표기화파향치료등도유적겁의의。 　　목적：감정인만성립세포백혈병세포주 K562중시부존재측군세포，병관찰측군세포아군여비측군세포아군중부분백세포분화항원적표체차이。 　　방법：채용류식세포술검측K562세포주중시부존재측군세포；병진일보분석K562측군세포화비측군세포량아군간CD34+、CD34+CD38-、CD34+CD38+、HLA-DR+세포적표체정황。 　　결과여결론：경Hoechst33342염색，류식세포의분석결과현시재K562중존재측군세포，저부분세포비례소，위(2.7±0.5)%；통계학분석측군세포화비측군세포아군중 CD34+、CD34+CD38-세포표체솔차이유현저성의의，이CD34+CD38+세포표체솔화HLA-DR+세포표체솔차이균무현저성의의；측군세포화비측군세포상비재분화항원표체상유이질성。
BACKGROUND:To study the phenotypes of side population cells in leukemia is important for understanding the heterogeneity and origin of tumor cells, molecular markers and targeted therapy. OBJECTIVE:To identify whether the human chronic myeloid leukemia cellline-K562 contains side population cells or not, and to further observe the differences in expressions of leukocyte differentiation antigens from side population cellsubset and non-side population cells subset. METHODS:Flow cytometry was used to detect whether there were side population cells in the K562 celllines. Then, the expression of CD34+, CD34+CD38-, CD34+CD38+, HLA-DR+cells in the side population subsets and non-side population subsets. RESULTS AND CONCLUSION:Flow cytometry results showed that the K562 cellline contained side population cells, and the proportion of side population cells was much lower. The side population cells accounted for (2.7±0.5)%of viable cells in K562. The expressions of CD34+cells and CD34+CD38-cells in the side population subset were significantly higher than those in the non-side population subsets. The expressions of CD34+CD38+cells and HLA-DR+cells in the side population subset and non-side population subset did not have a significant difference. Heterogeneity was found in the differentiation antigen expression between the side population subset and non-side population subset.