中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
49期
8570-8575
,共6页
干细胞%干细胞因子及调控因子%转化生长因子β3%脂肪间充质干细胞%纤维软骨%组织工程%干细胞图片文章
榦細胞%榦細胞因子及調控因子%轉化生長因子β3%脂肪間充質榦細胞%纖維軟骨%組織工程%榦細胞圖片文章
간세포%간세포인자급조공인자%전화생장인자β3%지방간충질간세포%섬유연골%조직공정%간세포도편문장
背景：颞下关节盘软骨缺损修复在口腔临床上仍然是较大的挑战，具有多向分化潜能的脂肪间充质干细胞为成纤维软骨类组织修复带来了希望。目前使用转化生长因子β3诱导脂肪间充质干细胞向纤维软骨细胞分化的研究很少。<br> 目的：观察转化生长因子β3对脂肪间充质干细胞生长形貌及向成纤维软骨分化的影响。<br> 方法：采用转化生长因子β3诱导SD大鼠脂肪间充质干细胞，观察成纤维软骨细胞分化的细胞形态，组织学和免疫荧光染色等方法检测脂肪间充质干细胞产生的细胞外基质Ⅰ，Ⅱ型胶原和蛋白多糖表达情况，评价脂肪间充质干细胞作为纤维软骨组织工程种子细胞的可行性。<br> 结果与结论：倒置荧光显微镜观察结果显示脂经转化生长因子β3生长因子诱导之后，细胞有明显的聚集生长现象，形态呈多角形、多边形，细胞外基质分泌增多。阿利新蓝染色结果表明，经转化生长因子β3诱导脂肪间充质干细胞显示明显的深蓝色，表明脂肪间充质干细胞合成了大量的糖胺聚糖。免疫染色结果表明，在转化生长因子β3诱导下，脂肪间充质干细胞合成Ⅰ，Ⅱ型胶原细胞外基质。提示转化生长因子β3可诱导脂肪间充质干细胞向成纤维软骨样细胞分化，也意味着脂肪间充质干细胞具有作为工程化纤维软骨种子细胞的潜能。
揹景：顳下關節盤軟骨缺損脩複在口腔臨床上仍然是較大的挑戰，具有多嚮分化潛能的脂肪間充質榦細胞為成纖維軟骨類組織脩複帶來瞭希望。目前使用轉化生長因子β3誘導脂肪間充質榦細胞嚮纖維軟骨細胞分化的研究很少。<br> 目的：觀察轉化生長因子β3對脂肪間充質榦細胞生長形貌及嚮成纖維軟骨分化的影響。<br> 方法：採用轉化生長因子β3誘導SD大鼠脂肪間充質榦細胞，觀察成纖維軟骨細胞分化的細胞形態，組織學和免疫熒光染色等方法檢測脂肪間充質榦細胞產生的細胞外基質Ⅰ，Ⅱ型膠原和蛋白多糖錶達情況，評價脂肪間充質榦細胞作為纖維軟骨組織工程種子細胞的可行性。<br> 結果與結論：倒置熒光顯微鏡觀察結果顯示脂經轉化生長因子β3生長因子誘導之後，細胞有明顯的聚集生長現象，形態呈多角形、多邊形，細胞外基質分泌增多。阿利新藍染色結果錶明，經轉化生長因子β3誘導脂肪間充質榦細胞顯示明顯的深藍色，錶明脂肪間充質榦細胞閤成瞭大量的糖胺聚糖。免疫染色結果錶明，在轉化生長因子β3誘導下，脂肪間充質榦細胞閤成Ⅰ，Ⅱ型膠原細胞外基質。提示轉化生長因子β3可誘導脂肪間充質榦細胞嚮成纖維軟骨樣細胞分化，也意味著脂肪間充質榦細胞具有作為工程化纖維軟骨種子細胞的潛能。
배경：섭하관절반연골결손수복재구강림상상잉연시교대적도전，구유다향분화잠능적지방간충질간세포위성섬유연골류조직수복대래료희망。목전사용전화생장인자β3유도지방간충질간세포향섬유연골세포분화적연구흔소。<br> 목적：관찰전화생장인자β3대지방간충질간세포생장형모급향성섬유연골분화적영향。<br> 방법：채용전화생장인자β3유도SD대서지방간충질간세포，관찰성섬유연골세포분화적세포형태，조직학화면역형광염색등방법검측지방간충질간세포산생적세포외기질Ⅰ，Ⅱ형효원화단백다당표체정황，평개지방간충질간세포작위섬유연골조직공정충자세포적가행성。<br> 결과여결론：도치형광현미경관찰결과현시지경전화생장인자β3생장인자유도지후，세포유명현적취집생장현상，형태정다각형、다변형，세포외기질분비증다。아리신람염색결과표명，경전화생장인자β3유도지방간충질간세포현시명현적심람색，표명지방간충질간세포합성료대량적당알취당。면역염색결과표명，재전화생장인자β3유도하，지방간충질간세포합성Ⅰ，Ⅱ형효원세포외기질。제시전화생장인자β3가유도지방간충질간세포향성섬유연골양세포분화，야의미착지방간충질간세포구유작위공정화섬유연골충자세포적잠능。
BACKGROUND:The repair of temporomandibular joint disc is stil a great clinical chal enge. Pluripotent adipose-derived mesenchymal stem cells bring a hope for fibrocartilage tissue repair. Up to now, there are few studies concerning transforming growth factor-β3 to induce differentiation of adipose-derived mesenchymal stem cells into fibrochondrocytes. <br> OBJECTIVE:To observe the growth and morphology of adipose-derived mesenchymal stem cells under the induction of transforming growth factor-β3. <br> METHODS:We observed the morphology of fibrochondrocytes differentiated from adipose-derived mesenchymal stem cells under the induction of transforming growth factor-β3. Histological and immunofluorescence staining were employed to detect the expression of extracellular matrix Ⅰ, Ⅱ col agens and proteoglycansthe produced by adipose-derived mesenchymal stem cells. We also evaluated the feasibility of adipose-derived mesenchymal stem cells as seed cells for fibrocartilage tissue engineering. <br> RESULTS AND CONCLUSION:Under the inverted fluorescence microscope, induced cells showed obvious aggregation growth, polygonal shape, and increased secretion of extracellular matrix. Alcian blue staining results showed that under the induction of transforming growth factor-β3, adipose-derived mesenchymal stem cells exhibited the distinctly deep blue, indicating a large number of glycosaminoglycan was synthesized. Immunostaining results showed that under the induction of transforming growth factorβ3, adipose-derived mesenchymal stem cells synthesized extracellular matrix Ⅰ, Ⅱ col agen. These findings suggest that transforming growth factorβ3 can induce adipose-derived mesenchymal stem cells to differentiate into fibrouscartilage-like cells, and that adipose-derived mesenchymal stem cells can serve as seed cells for fibrocartilage tissue engineering.
