CAJ | 학술논문

背景：研究表明，人胎盘来源间充质干细胞来源广泛，增殖能力强，其移植对腱-骨愈合有明显的促进作用。 　　目的：进一步验证人胎盘来源间充质干细胞对骨-肌腱结合处愈合的影响。 　　方法：采用贴壁分离筛选法获取人胎盘来源间充质干细胞。30只雄性SD大鼠随机分为2组各15只。建立大鼠骨-肌腱损伤模型，实验组在骨-肌腱结合面注入人胎盘来源间充质干细胞，对照组只注入生理盐水。 　　结果与结论：实验组人胎盘间充质干细胞移植后2，4，6周显微镜观察在腱骨界面有干细胞存在并有较多血管组织再生，并且有部分纤维软骨增生。在移植后4，6周实验组最大拔出载荷显著高于对照组(P <0.05)。说明人胎盘来源充质干细胞可以促进骨-肌腱结合部的早期愈合，提高其生物力学强度。
배경：연구표명，인태반래원간충질간세포래원엄범，증식능력강，기이식대건-골유합유명현적촉진작용。 　　목적：진일보험증인태반래원간충질간세포대골-기건결합처유합적영향。 　　방법：채용첩벽분리사선법획취인태반래원간충질간세포。30지웅성SD대서수궤분위2조각15지。건립대서골-기건손상모형，실험조재골-기건결합면주입인태반래원간충질간세포，대조조지주입생리염수。 　　결과여결론：실험조인태반간충질간세포이식후2，4，6주현미경관찰재건골계면유간세포존재병유교다혈관조직재생，병차유부분섬유연골증생。재이식후4，6주실험조최대발출재하현저고우대조조(P <0.05)。설명인태반래원충질간세포가이촉진골-기건결합부적조기유합，제고기생물역학강도。
BACKGROUND:Studies have shown that human placenta-derived mesenchymal stem cells with strong proliferative ability have rich sources and can remarkably promote tendon-bone healing after celltransplantation. OBJECTIVE:To observe the effect of human placenta-derived mesenchymal stem cells on tendon-bone healing in a bone tunnel. METHODS:Human placenta-derived mesenchymal stem cells were separated using adherent separation screening method. Thirty 8-week-old male Sprague-Dawley rats were randomly divided into two groups, 15 rats as experimental group and 15 rats as control group. Experimental group were subjected to transplantation of human placenta-derived mesenchymal stem cells and the control group were injected with saline solution. RESULTS AND CONCLUSION:Stem cells, new vessels, and fibrocartilage hyperplasia were observed on the tendon-bone interface with microscope at 2, 4 and 6 weeks after celltransplantation in the experimental group. Biomechanical y, the maximum pul out load in the experimental group was significantly higher than that in the control group at 4 and 6 weeks after celltransplantation (P<0.05). These findings suggest that human placenta-derived mesenchymal stem cells can accelerate early tendon-bone healing in a bone tunnel and strengthen the biomechanical strength.