CAJ | 학술논문

背景：课题组前期实验发现改良富血小板血浆对人牙髓细胞增殖的促进作用具有浓度依赖性，以10%浓度尤为明显。 　　目的：观察不同浓度改良富血小板血浆对人骨髓间充质干细胞体外生长增殖的作用效果，以及最佳浓度改良富血小板血浆对骨髓间充质干细胞免疫原性的影响。 　　方法：使用健康志愿者捐献骨髓血培养的第三四代人骨髓间充质干细胞，行流式细胞术及成脂、矿化诱导分化鉴定。将由同一志愿者采集的静脉血制备的改良富血小板血浆作用于人骨髓间充质干细胞，以CCK-8法测定细胞增殖变化，绘制生长曲线。选取最适合人骨髓间充质干细胞增殖的改良富血小板血浆浓度，以体积分数10%FBS培养基为对照，通过流式细胞术测定连续培养3代的人骨髓间充质干细胞表面Stro-1的表达率。 　　结果与结论：5%-10%的改良富血小板血浆在培养第6，8天均显著促进了骨髓间充质干细胞的增殖，其中以10%浓度组尤为显著在培养第10天仍显著促进了骨髓间充质干细胞的增殖且细胞表面Stro-1的表达率连续3代均高于对照组。说明改良富血小板血浆对人骨髓间充质干细胞增殖的促进作用具有浓度依赖性，10%改良富血小板血浆可以较好地保持人骨髓间充质干细胞的免疫原性。
배경：과제조전기실험발현개량부혈소판혈장대인아수세포증식적촉진작용구유농도의뢰성，이10%농도우위명현。 　　목적：관찰불동농도개량부혈소판혈장대인골수간충질간세포체외생장증식적작용효과，이급최가농도개량부혈소판혈장대골수간충질간세포면역원성적영향。 　　방법：사용건강지원자연헌골수혈배양적제삼사대인골수간충질간세포，행류식세포술급성지、광화유도분화감정。장유동일지원자채집적정맥혈제비적개량부혈소판혈장작용우인골수간충질간세포，이CCK-8법측정세포증식변화，회제생장곡선。선취최괄합인골수간충질간세포증식적개량부혈소판혈장농도，이체적분수10%FBS배양기위대조，통과류식세포술측정련속배양3대적인골수간충질간세포표면Stro-1적표체솔。 　　결과여결론：5%-10%적개량부혈소판혈장재배양제6，8천균현저촉진료골수간충질간세포적증식，기중이10%농도조우위현저재배양제10천잉현저촉진료골수간충질간세포적증식차세포표면Stro-1적표체솔련속3대균고우대조조。설명개량부혈소판혈장대인골수간충질간세포증식적촉진작용구유농도의뢰성，10%개량부혈소판혈장가이교호지보지인골수간충질간세포적면역원성。
BACKGROUND:Previous experiments have shown that improved platelet-rich plasma can promote the proliferation of human dental pulp cells in a concentration-dependent manner, particularly when the concentration is 10%. OBJECTIVE:To investigate the effect of platelet-rich plasma at different concentrations on the proliferation and immunogenicity of human bone marrow mesenchymal stem cells. METHODS:Human bone marrow mesenchymal stem cells from healthy donors were cultured and passaged for 3-4 passages, identified by flow cytometry and differentiation inductions. Platelet-rich plasma samples which were manufactured from the venous blood of the same donor were used for culturing human bone marrow mesenchymal stem cells. The proliferation of human bone marrow mesenchymal stem cells was measured by cellcounting kit-8 method and the growth curves were drawn. The most suitable concentration of platelet-rich plasma was selected to culture human bone marrow mesenchymal stem cells for three generations and the Stro-1 expression rate on the surface of human bone marrow mesenchymal stem cells was determined through flow cytometry. RESULTS AND CONCLUSION:Platelet-rich plasma at the concentration of 5%-10%evidently promoted the proliferation of human bone marrow mesenchymal stem cells on the 6th and 8th days. The most effective concentration to promote the proliferation was 10%. Platelet-rich plasma at the concentration of 10%stil promoted the proliferation of human bone marrow mesenchymal stem cells on the 10th day, and maintained a better immunogenicity of human bone marrow mesenchymal stem cells compared to the control group. These findings indicate that platelet-rich plasma can promote the proliferation of human bone marrow mesenchymal stem cells in a concentration-dependent manner, and 10%platelet-rich plasma is better to maintain the immunogenicity of human bone marrow mesenchymal stem cells.