CAJ | 학술논문

背景：骨髓间充质干细胞具备化学趋向性和归巢作用，对于促进患者免疫系统的重建、消除残留病灶及防止复发转移具有良好的效果。 　　目的：观察人骨髓间充质干细胞移植入动物模型对肝癌组织的影响情况及对肝癌转移潜能影响。 　　方法：制作高转移潜能动物模型，实验组在接种肿瘤后第7日开始尾静脉注射骨髓间充质干细胞，5×105/次，2次/周；对照组尾静脉注射骨髓间充质干细胞培养液，0.2 mL/次，实验开始后每4 d用测量肿瘤体积，肿瘤接种后14 d(2周)，21 d(3周)，28 d(4周)，35 d(5周)，42 d(6周)处死动物，称质量，取瘤块，称瘤质量，计算肿瘤质量抑制率。PCR检测动物模型标本转移相关因子骨桥蛋白、骨唾液蛋白、整合素αⅤ基因的表达，及肿瘤标本凋亡相关因子bcl-2、bax、caspase3基因的表达。 　　结果与结论：第3周时肿瘤的质量抑制率效果最好，随着时间的延长，肿瘤的抑制率逐渐下降。肝癌转移相关的生物学指标均呈逐渐下降趋势，代表肿瘤凋亡指标的因子呈两极分化表现，抗凋亡bcl-2因子呈逐渐下降的趋势，凋亡因子bax、caspase3呈逐渐升高的趋势。骨髓间充质干细胞对肝癌动物模型肿瘤抑制效能随时间而变化，骨髓间充质干细胞移植后第3周对肝癌抑制效果最显著，随着时间的延长，抑制效能减弱。骨髓间充质干细胞对肝癌的转移潜能具有抑制作用。
배경：골수간충질간세포구비화학추향성화귀소작용，대우촉진환자면역계통적중건、소제잔류병조급방지복발전이구유량호적효과。 　　목적：관찰인골수간충질간세포이식입동물모형대간암조직적영향정황급대간암전이잠능영향。 　　방법：제작고전이잠능동물모형，실험조재접충종류후제7일개시미정맥주사골수간충질간세포，5×105/차，2차/주；대조조미정맥주사골수간충질간세포배양액，0.2 mL/차，실험개시후매4 d용측량종류체적，종류접충후14 d(2주)，21 d(3주)，28 d(4주)，35 d(5주)，42 d(6주)처사동물，칭질량，취류괴，칭류질량，계산종류질량억제솔。PCR검측동물모형표본전이상관인자골교단백、골타액단백、정합소αⅤ기인적표체，급종류표본조망상관인자bcl-2、bax、caspase3기인적표체。 　　결과여결론：제3주시종류적질량억제솔효과최호，수착시간적연장，종류적억제솔축점하강。간암전이상관적생물학지표균정축점하강추세，대표종류조망지표적인자정량겁분화표현，항조망bcl-2인자정축점하강적추세，조망인자bax、caspase3정축점승고적추세。골수간충질간세포대간암동물모형종류억제효능수시간이변화，골수간충질간세포이식후제3주대간암억제효과최현저，수착시간적연장，억제효능감약。골수간충질간세포대간암적전이잠능구유억제작용。
BACKGROUND:Bone marrow mesenchymal stem cells have the chemotaxis and homing role that promotes immune system reconstruction, eliminate residual lesions and prevent recurrence in patients. OBJECTIVE:To observe therapeutic effect of human bone marrow mesenchymal stem cells transplantation into high-metastatic potential hepatocellular carcinoma animal models on metastatic potential of high-metastatic potential hepatocellular carcinoma. METHODS:Nude mouse models of high-metastatic potential hepatocellular carcinoma were established in vivo. In the experimental group, 5×105 cells were injected via the tail vein on day 7 after tumor inoculation, twice a week. In the control group, cellculture medium, 0.2 mL per mouse, was injected by the tail vein. After the start of the experiment, tumor volume was measured every 4 days. After tumor inoculation for 14 days, 21 days, 28 days, 35 days, 42 days, animal models were sacrificed, and then tumor mass and body mass were recorded to calculate the inhibition rate. PCR was employed to detect osteopontin, bone sialoprotein, and integrinα Ⅴ mRNA expression, as wel as bcl-2, bax, caspase3 mRNA expression. RESULTS AND CONCLUSION:The inhibition rate of tumor mass showed the best results in week 3. As time went on, the tumor inhibition rate was gradual y decreased. Metastasis-related biological factors showed a gradual down-regulated trend, indicating the polarization of tumor apoptotic indexes, that is, anti-apoptotic factor, bcl-2, showed a decreasing trend, while apoptotic factors, bax and caspase3, appeared to have a gradual y increased trend. These findings suggest that human bone marrow mesenchymal stem cells effects to inhibit high-metastatic potential hepatocellular carcinoma animal models appear to vary with time. After human bone marrow mesenchymal stem cells transplantation for 3 weeks, the inhibition performance on high-metastatic potential hepatocellular carcinoma is the best, and then it weakens with time. Human bone marrow mesenchymal stem cells are found to inhibit the metastatic potential of hepatocellular carcinoma.