中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
49期
8488-8497
,共10页
毕凌云%杨达胜%赵德安%梁斌%张瑞霞%白海涛
畢凌雲%楊達勝%趙德安%樑斌%張瑞霞%白海濤
필릉운%양체성%조덕안%량빈%장서하%백해도
干细胞%骨髓干细胞%肾损伤%干细胞因子%粒细胞集落刺激因子%肾缺血再灌注损伤%细胞凋亡%省级基金%干细胞图片文章
榦細胞%骨髓榦細胞%腎損傷%榦細胞因子%粒細胞集落刺激因子%腎缺血再灌註損傷%細胞凋亡%省級基金%榦細胞圖片文章
간세포%골수간세포%신손상%간세포인자%립세포집락자격인자%신결혈재관주손상%세포조망%성급기금%간세포도편문장
背景:骨髓干细胞具有多项分化潜能,可分化为肾组织固有细胞、修复损伤肾组织。<br> 目的:探讨粒细胞集落刺激因子联合干细胞因子动员自身骨髓干细胞对大鼠缺血再灌注肾损伤细胞凋亡与增殖的影响。<br> 方法:160只大鼠尿筛阴性后随机均分为正常对照组、模型组、细胞因子治疗组、治疗对照组。模型组和细胞因子治疗组建立大鼠单侧肾脏缺血再灌注损伤模型;细胞因子治疗组和治疗对照组于造模后24 h开始皮下注射粒细胞集落刺激因子(50μg/kg,1次/d)和干细胞因子(200μg/kg,1次/d),连续5 d;模型组不给药,正常对照组不予干预。TUNEL法检测细胞凋亡;免疫组织化学法(SABC法)检测肾组织CD34+细胞、Caspase-3、Bcl-2、细胞增殖核抗原表达情况。<br> 结果与结论:细胞因子治疗组肾组织内CD34+细胞较正常对照组、模型组明显增多(P<0.05)。不同时间点模型组和细胞因子治疗组凋亡指数、Capase-3表达量均高于正常对照组和治疗对照组(P <0.05),且模型组均显著高于细胞因子治疗组(P<0.05)。不同时间点模型组和细胞因子治疗组Bcl-2阳性表达细胞均高于正常对照组和治疗对照组(P<0.05)。细胞因子治疗组显著高于模型组,然后随着时间推移Bcl-2表达量明显减少(P<0.05)。模型组和细胞因子治疗组均可见细胞增殖核抗原阳性表达细胞;模型组于第24天增殖指数达峰值,后逐渐下降。细胞因子治疗组在第10天即达到高峰,持续至第17天,然后逐渐下降。说明粒细胞集落刺激因子联合干细胞因子动员自身骨髓干细胞可以促进肾缺血再灌注损伤后肾小管上皮细胞的增殖和减少细胞凋亡,从而有利于肾小管损伤的恢复。
揹景:骨髓榦細胞具有多項分化潛能,可分化為腎組織固有細胞、脩複損傷腎組織。<br> 目的:探討粒細胞集落刺激因子聯閤榦細胞因子動員自身骨髓榦細胞對大鼠缺血再灌註腎損傷細胞凋亡與增殖的影響。<br> 方法:160隻大鼠尿篩陰性後隨機均分為正常對照組、模型組、細胞因子治療組、治療對照組。模型組和細胞因子治療組建立大鼠單側腎髒缺血再灌註損傷模型;細胞因子治療組和治療對照組于造模後24 h開始皮下註射粒細胞集落刺激因子(50μg/kg,1次/d)和榦細胞因子(200μg/kg,1次/d),連續5 d;模型組不給藥,正常對照組不予榦預。TUNEL法檢測細胞凋亡;免疫組織化學法(SABC法)檢測腎組織CD34+細胞、Caspase-3、Bcl-2、細胞增殖覈抗原錶達情況。<br> 結果與結論:細胞因子治療組腎組織內CD34+細胞較正常對照組、模型組明顯增多(P<0.05)。不同時間點模型組和細胞因子治療組凋亡指數、Capase-3錶達量均高于正常對照組和治療對照組(P <0.05),且模型組均顯著高于細胞因子治療組(P<0.05)。不同時間點模型組和細胞因子治療組Bcl-2暘性錶達細胞均高于正常對照組和治療對照組(P<0.05)。細胞因子治療組顯著高于模型組,然後隨著時間推移Bcl-2錶達量明顯減少(P<0.05)。模型組和細胞因子治療組均可見細胞增殖覈抗原暘性錶達細胞;模型組于第24天增殖指數達峰值,後逐漸下降。細胞因子治療組在第10天即達到高峰,持續至第17天,然後逐漸下降。說明粒細胞集落刺激因子聯閤榦細胞因子動員自身骨髓榦細胞可以促進腎缺血再灌註損傷後腎小管上皮細胞的增殖和減少細胞凋亡,從而有利于腎小管損傷的恢複。
배경:골수간세포구유다항분화잠능,가분화위신조직고유세포、수복손상신조직。<br> 목적:탐토립세포집락자격인자연합간세포인자동원자신골수간세포대대서결혈재관주신손상세포조망여증식적영향。<br> 방법:160지대서뇨사음성후수궤균분위정상대조조、모형조、세포인자치료조、치료대조조。모형조화세포인자치료조건립대서단측신장결혈재관주손상모형;세포인자치료조화치료대조조우조모후24 h개시피하주사립세포집락자격인자(50μg/kg,1차/d)화간세포인자(200μg/kg,1차/d),련속5 d;모형조불급약,정상대조조불여간예。TUNEL법검측세포조망;면역조직화학법(SABC법)검측신조직CD34+세포、Caspase-3、Bcl-2、세포증식핵항원표체정황。<br> 결과여결론:세포인자치료조신조직내CD34+세포교정상대조조、모형조명현증다(P<0.05)。불동시간점모형조화세포인자치료조조망지수、Capase-3표체량균고우정상대조조화치료대조조(P <0.05),차모형조균현저고우세포인자치료조(P<0.05)。불동시간점모형조화세포인자치료조Bcl-2양성표체세포균고우정상대조조화치료대조조(P<0.05)。세포인자치료조현저고우모형조,연후수착시간추이Bcl-2표체량명현감소(P<0.05)。모형조화세포인자치료조균가견세포증식핵항원양성표체세포;모형조우제24천증식지수체봉치,후축점하강。세포인자치료조재제10천즉체도고봉,지속지제17천,연후축점하강。설명립세포집락자격인자연합간세포인자동원자신골수간세포가이촉진신결혈재관주손상후신소관상피세포적증식화감소세포조망,종이유리우신소관손상적회복。
BACKGROUND:Bone marrow stem cells are defined by their multi-potential ability, and can be differentiated into intrinsic cells in the kidney. <br> OBJECTIVE:To study the effects of mobilizing autologous bone marrow stem cells by granulocyte colony-stimulating factor plus stem cellfactor on cellapoptosis and proliferation of rats with renal ischemia-reperfusion injury. <br> METHODS:Total y 160 male Sprague-Dawley rats were randomly divided into four groups:control group, model group, cytokine treatment group, cytokine control group. Rat models of unilateral renal ischemia-reperfusion injury were established in the model and cytokine treatment groups. Rats in the cytokine treatment group and cytokine control group received subcutaneous injection of granulocyte colony-stimulating factor (50μg/kg) and stem cellfactor (200μg/kg), once a day, for 5 continuous days. Rats in the model and control groups had no treatment. Apoptotic cells were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling method, and the expression of CD34-positive cells, Caspase-3, Bcl-2, proliferating cellnuclear antigen in the kidney were measured using immunohistochemistry staining. <br> RESULTS AND CONCLUSION:The number of CD34-positive cells in renal tissue of the cytokine treatment group was significantly higher than that of the control group and model group (P<0.05). The apoptotic index and expression of Capase-3 in the model group and cytokine treatment group were higher than those in the control group and cytokine control group (P<0.05). The apoptotic index and expression of Capase-3 in the cytokine treatment group were lower than that in the model group (P<0.05). The expression of Bcl-2 in the model group and cytokine treatment group was higher than that in the control group and cytokine control group (P<0.05). The expression of Bcl-2 in the cytokine treatment group was higher than that in the model group (P<0.05);however, as time went on, Bcl-2 expression was obviously decreased. Proliferating cellnuclear antigen expressed both in the model group and in the cytokine treatment group. Additional y, the proliferative index reached peak at 24 days in the model group, and then decreased gradual y;while in the cytokine treatment group, it reached the peak at 10 days, maintained a high level until the 17th day, and then decreased gradual y. Mobilization of autologous bone marrow stem cells by combination of granulocyte colony-stimulating factor and stem cellfactor can increase proliferation and decrease apoptosis of renal tubular epithelial cells after renal ischemia-reperfusion injury, and thus, promote the recovery from renal tubular injury.