CAJ | 학술논문

背景：生物活性玻璃是一种多相复合材料，具有良好的生物活性、骨传导性及生物相容性，但作为骨修复材料仍然存在不能完全降解、机械强度较低等不足。 　　目的：设计生物活性玻璃/壳聚糖复合材料骨组织工程支架，并检测其理化性能。 　　方法：将2.0%壳聚糖盐酸溶液与β-甘油磷酸钠以7∶1的体积比混合制备壳聚糖溶液。称取0.5，1.0，1.5 g生物活性玻璃分别加入上述壳聚糖溶液中，使得壳聚糖与生物活性玻璃的质量比为2∶1，1∶1及1∶1.5。将复合材料浸泡于模拟生理体液中7d进行体外矿化。 　　结果与结论：扫描电镜见复合支架具有相互贯通的多孔结构，孔隙率最高可达89%，孔径大小合适，为100-300μm，生物活性玻璃以针状形式分散在壳聚糖支架之间，均匀排列，被壳聚糖支架充分包裹结合紧密。随生物活性玻璃含量的增加，复合材料的孔隙率逐渐下降，断裂强度逐渐升高，他们之间呈正相关性。X 射线衍射图及傅里叶变换红外光谱证实复合支架中的单一材料未发生性质改变，示差扫描量热法分析显示正常体温情况下材料无质量丢失。矿化3d后材料表面形成的羟基磷灰石逐渐长大为绒毛状，数量也明显增多；矿化7d后绒毛状的羟基磷灰石长成为针状，数量进一步增多，且众多的矿化物结成球状。
배경：생물활성파리시일충다상복합재료，구유량호적생물활성、골전도성급생물상용성，단작위골수복재료잉연존재불능완전강해、궤계강도교저등불족。 　　목적：설계생물활성파리/각취당복합재료골조직공정지가，병검측기이화성능。 　　방법：장2.0%각취당염산용액여β-감유린산납이7∶1적체적비혼합제비각취당용액。칭취0.5，1.0，1.5 g생물활성파리분별가입상술각취당용액중，사득각취당여생물활성파리적질량비위2∶1，1∶1급1∶1.5。장복합재료침포우모의생리체액중7d진행체외광화。 　　결과여결론：소묘전경견복합지가구유상호관통적다공결구，공극솔최고가체89%，공경대소합괄，위100-300μm，생물활성파리이침상형식분산재각취당지가지간，균균배렬，피각취당지가충분포과결합긴밀。수생물활성파리함량적증가，복합재료적공극솔축점하강，단렬강도축점승고，타문지간정정상관성。X 사선연사도급부리협변환홍외광보증실복합지가중적단일재료미발생성질개변，시차소묘량열법분석현시정상체온정황하재료무질량주실。광화3d후재료표면형성적간기린회석축점장대위융모상，수량야명현증다；광화7d후융모상적간기린회석장성위침상，수량진일보증다，차음다적광화물결성구상。
BACKGROUND:Bioactive glass, a multi-phase composite material, has good biological activity, bone conductivity and biocompatibility, but as a bone repair material it cannot be completely degraded, and has low mechanical strength that is insufficient. OBJECTIVE:To design a kind of bioactive glasses/chitosan composite scaffold, and to investigate its physicochemical properties and cellcompatibility. METHODS:Hydrochloric acid solution containing 2.0%chitosan was mixed withβ-glycerophosphate at a radio of 7:1 to prepare chitosan solution. Bioactive glasses of 0.5, 1.0, 1.5 g were added into the prepared chitosan solution, and the mass ratios of chitosan and bioactive glass were 2:1, 1:1, and 1:1.5 respectively. The composite materials were immersed and mineralized in simulated body fluid for 7 days. RESULTS AND CONCLUSION:Scanning electron microscopy showed that the composite scaffold had an interconnected porous structure with the porosity of 89%and the pore size of 100-300μm;bioactive glasses dispersed in a needle shape between the chitosan scaffolds, arranged evenly, and were ful y wrapped tightly by the scaffolds. With the increase in mass of bioactive glass, the porosity of the composites decreased, but the fracture strength gradual y increased. There was a positive correlation between the composite porosity and fracture strength. X-ray diffraction and Fourier transform infrared spectroscopy confirmed that the composite scaffold appeared to have no changes in the nature of single materials, and differential scanning calorimetry analysis showed no mass loss at normal body temperature. After 3 days of mineralization, hydroxyapatite forming on the material surface gradual y grew up as a vil ous shape, and also significantly increased in number. After 7 days of mineralization, hydroxyapatite changed from a vil ous shape to a needle shape, the amount of hydroxyapatite was increased further, and many mineralized products were in a spherical shape.