CAJ | 학술논문

背景：前期实验利用脉冲激光沉积方法制作了胶原包埋羟基磷灰石涂层人工机械瓣膜。 　　目的：进一步分析胶原包埋羟基磷灰石涂层人工机械瓣膜的组织相容性和毒性。 　　方法：将传代的犬血管内皮细胞悬液接种在胶原包埋羟基磷灰石涂层人工机械瓣膜材料上，1组置于含体积分数5%CO2、37℃恒温培养箱内静态培养3周，另1组置于含体积分数5%CO2、37℃恒温培养箱动态旋转培养3周，扫描电子显微镜下观察细胞在材料上的附着情况。采用MTT法测定血管内皮细胞与胶原包埋羟基磷灰石涂层人工机械瓣膜材料复合培养的增殖能力。 　　结果与结论：动态旋转培养中见人工机械瓣膜材料的表面有细胞附着，并且数目比静态培养多，细胞分布均匀，在培养第21天可见均匀细胞融合成片，将材料表面均匀覆盖；在静态培养中细胞成堆，分布不均匀。人工机械瓣膜材料对犬血管内皮细胞增殖无明显影响，细胞生长良好。表明胶原包埋羟基磷灰石涂层人工机械瓣膜材料对血管内皮细胞的增殖无明显影响，无明显毒性，具有良好的生物相容性。
배경：전기실험이용맥충격광침적방법제작료효원포매간기린회석도층인공궤계판막。 　　목적：진일보분석효원포매간기린회석도층인공궤계판막적조직상용성화독성。 　　방법：장전대적견혈관내피세포현액접충재효원포매간기린회석도층인공궤계판막재료상，1조치우함체적분수5%CO2、37℃항온배양상내정태배양3주，령1조치우함체적분수5%CO2、37℃항온배양상동태선전배양3주，소묘전자현미경하관찰세포재재료상적부착정황。채용MTT법측정혈관내피세포여효원포매간기린회석도층인공궤계판막재료복합배양적증식능력。 　　결과여결론：동태선전배양중견인공궤계판막재료적표면유세포부착，병차수목비정태배양다，세포분포균균，재배양제21천가견균균세포융합성편，장재료표면균균복개；재정태배양중세포성퇴，분포불균균。인공궤계판막재료대견혈관내피세포증식무명현영향，세포생장량호。표명효원포매간기린회석도층인공궤계판막재료대혈관내피세포적증식무명현영향，무명현독성，구유량호적생물상용성。
BACKGROUND:In early experiments, we prepared hydroxyapatite-coated mechanical heart valve embedded with col agen using impulse laser sediment method. OBJECTIVE:To further analyze the histocompatibility and toxicity of hydroxyapatite-coated mechanical heart valve embedded with col agen. METHODS:After passage, canine vascular endothelial cellsuspension was inoculated onto the hydroxyapatite-coated mechanical heart valve embedded with col agen. One group was inoculated in 5%CO2, 37 ℃ incubator for 3 weeks static culture, and the other group was inoculated in 5%CO 2 , 37 ℃ incubator for 3 weeks spinner culture. Scanning electron microscope was used to observe cellattachment on the material. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to the proliferative capacity of vascular endothelial cells cultured with the hydroxyapatite-coated mechanical heart valve embedded with col agen. RESULTS AND CONCLUSION:During the spinner culture, adherent cells were found on the surface of mechanical heart valve, and the cells distributed evenly and confluent at 21 days to cover the surface of the material. The number of adherent cells in the spinner culture was higher than that in the static culture. The cells during the static culture were aggregated and distributed irregularly. The mechanical heart valve exhibited no effects on the proliferation of canine vascular endothelial cells which grew wel . These findings indicate that the hydroxyapatite-coated mechanical heart valve embedded with col agen exert no effect on proliferation of vascular endothelial cells, has no toxicity and has good biocompatibility.